DPPA2 / PESCRG1 antibody
Principal name
DPPA2 / PESCRG1 antibody
Alternative names for DPPA2 / PESCRG1 antibody
Developmental pluripotency-associated protein 2, Pluripotent embryonic stem cell-related gene 1 protein
Ncbi ID
9606, NP_620170, NP_620170.3, NM_138815, NP_082891.1, NM_028615
Available reactivities
Available hosts
Available applications
ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of DPPA2 / PESCRG1 antibody
- Kit Component:
- - KN304787G1, Dppa2 gRNA vector 1 in pCas-Guide vector
- KN304787G2, Dppa2 gRNA vector 2 in pCas-Guide vector
- KN304787D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Du,J., (2004) Zhonghua Yi Xue Yi Chuan Xue Za Zhi 21 (6), 542-547
Western Blot analysis of DPPA2 expression in transfected 293T cell line (H00151871-T01) by DPPA2 MaxPab polyclonal antibody.Lane 1: DPPA2 transfected lysate(32.78 KDa).Lane 2: Non-transfected lysate.
DPPA2 MaxPab polyclonal antibody. Western Blot analysis of DPPA2 expression in Jurkat.
Western Blot analysis of DPPA2 expression in transfected 293T cell line (H00151871-T02) by DPPA2 MaxPab polyclonal antibody.Lane 1: DPPA2 transfected lysate(32.78 KDa).Lane 2: Non-transfected lysate.
DPPA2 MaxPab polyclonal antibody. Western Blot analysis of DPPA2 expression in Jurkat.
Human HepG2; WB Suggested Anti-DPPA2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; DPPA2 antibody - N-terminal region (ARP52662_P050) in Human HepG2 cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DPPA2 (RC205441, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DPPA2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-DPPA2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Predicted cell location: Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using DPPA2 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted cell location: Nucleus. Positive control: Human colon cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using DPPA2 antibody at dilution 1/25, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-DPPA2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA321929 | DPPA2 / PESCRG1 antibody |
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| Anti-DPPA2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA321930 | DPPA2 / PESCRG1 antibody |
||||||
| Anti-DPPA2 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA340320 | DPPA2 / PESCRG1 antibody |
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| Rabbit Polyclonal Anti-DPPA2 Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Rt | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA800361 | DPPA2 / PESCRG1 antibody |
||||||
| DPPA2 mouse monoclonal antibody, clone OTI1G10 (formerly 1G10) | |||||||
 |
Mouse | IgG1 | OTI1G10 | Purified | Can, Hu, Rt | WB |
0.1 ml /
€379.00
|
| OriGene Technologies, Inc. | |||||||
 +1 additional image |
|
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CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN205441 | DPPA2 - human gene knockout kit via CRISPR |
||
 |
Human | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN205441BN | DPPA2 - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205441LP | DPPA2 - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN205441RB | DPPA2 - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN304787 | Dppa2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,200.00
|
| OriGene Technologies, Inc. | |||
| KN304787BN | Dppa2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN304787LP | Dppa2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN304787RB | Dppa2 - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Lysates
| Catalog No. | ||
|---|---|---|
| LY403369 | DPPA2 overexpression lysate |
|
|
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
Human Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| RC205441L2V | Lenti ORF particles, DPPA2 (mGFP-tagged) - Human developmental pluripotency associated 2 (DPPA2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€960.00
|
|
| OriGene Technologies, Inc. | ||
| RC205441L1V | Lenti ORF particles, DPPA2 (Myc-DDK tagged) - Human developmental pluripotency associated 2 (DPPA2) , 200 uL, >10^7 TU/mL |
|
|
200 µl /
€860.00
|
|
| OriGene Technologies, Inc. | ||
Mouse Lenti ORF Particles
| Catalog No. | ||
|---|---|---|
| MR224204L2V | Lenti ORF particles, Dppa2 (GFP-tagged) - Mouse developmental pluripotency associated 2 (Dppa2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€880.00
|
|
| OriGene Technologies, Inc. | ||
| MR224204L1V | Lenti ORF particles, Dppa2 (Myc-DDK-tagged) - Mouse developmental pluripotency associated 2 (Dppa2), 200 uL, >10^7 TU/mL |
|
|
200 µl /
€780.00
|
|
| OriGene Technologies, Inc. | ||
