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DRAP1 antibody

Principal name

DRAP1 antibody

Alternative names for DRAP1 antibody

Dr1-associated corepressor, Dr1-associated protein 1, NC2-alpha, Negative co-factor 2-alpha

SwissProt ID

A0JPP1 (Rat), Q14919 (Human), Q2YDP3 (Bovin), Q9D6N5 (Mouse)

Gene ID

10589 (DRAP1)

Ncbi ID

9606, NP_006433.2, NP_006433, NM_006442

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of DRAP1 antibody

Transcriptional repression is a general mechanism for regulating transcriptional initiation in organisms ranging from yeast to humans. Accurate initiation of transcription from eukaryotic protein-encoding genes requires the assembly of a large multiprotein complex consisting of RNA polymerase II and general transcription factors such as TFIIA, TFIIB, and TFIID. DR1 is a repressor that interacts with the TATA-binding protein (TBP) of TFIID and prevents the formation of an active transcription complex by precluding the entry of TFIIA and/or TFIIB into the preinitiation complex. The protein encoded by this gene is a corepressor of transcription that interacts with DR1 to enhance DR1-mediated repression. The interaction between this corepressor and DR1 is required for corepressor function and appears to stabilize the TBP-DR1-DNA complex. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

DRAP1 polyclonal antibody (A01), Lot # 051019JCO1 Western Blot analysis of DRAP1 expression in A-431 ( Cat # L015V1 ).

H00010589-A01

Immunohistochemical of paraffin-embedded human pancreas cancer using 11020-1-AP(DRAP1 antibody) at dilution of 1:50 (under 10x lens)

11020-1-AP

Immunofluorescent analysis of HepG2 cells, using DRAP1 antibody 11020-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

11020-1-AP

Human brain tissue were subjected to SDS PAGE followed by western blot with 11020-1-AP(DRAP1 antibody) at dilution of 1:300

11020-1-AP

Human heart tissue were subjected to SDS PAGE followed by western blot with 11020-1-AP(DRAP1 antibody) at dilution of 1:300

11020-1-AP

Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11020-1-AP(DRAP1 antibody) at dilution of 1:300

11020-1-AP

Western Blot: DRAP1 Lysate [NBL1-10014] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for DRAP1

NBL1-10014

Human Stomach; WB Suggested Anti-DRAP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Stomach; DRAP1 antibody - N-terminal region (ARP31488_P050) in Human Stomach cells using Western Blot

ARP31488_P050

Immunohistochemistry-Paraffin: DRAP1 Antibody [NBP1-81008] - Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.

NBP1-81008

Western Blot: DRAP1 Antibody [NBP1-81008] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

NBP1-81008

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
H00010589-A01	DRAP1 antibody
	Mouse		Serum	Hu	E, WB	50 µl / €290.00
						Abnova Taiwan Corp.
ARP31488_P050	DRAP1 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	WB	50 µg / €350.00
						AVIVA Systems Biology
NBP1-81008	DRAP1 antibody
	Rabbit	IgG	Aff - Purified	Hu	P, WB	0.1 ml / €500.00
						Novus Biologicals Inc.
+1 additional image

Proteins & Growth Factors

Catalog No.	Species	Pres.	Purity	Source
TP304031	DRAP1
	Human		> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €680.00
					OriGene Technologies, Inc.
H00010589-P01-10	DRAP1
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.		10 µg / €380.00
					Abnova Taiwan Corp.
H00010589-P01-25	DRAP1
	Human	Purified	12.5% SDS-PAGE Stained with Coomassie Blue.		25 µg / €510.00
					Abnova Taiwan Corp.
H00010589-Q01-10	DRAP1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.	10 µg / €380.00
					Abnova Taiwan Corp.
H00010589-Q01-25	DRAP1
	Human		12.5% SDS-PAGE Stained with Coomassie Blue.	in vitro transl.	25 µg / €510.00
					Abnova Taiwan Corp.

Lysates

Catalog No.
LY401939	DRAP1 overexpression lysate
		0.1 mg / €280.00
		OriGene Technologies, Inc.

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