Draxin antibody

Principal name

Draxin antibody

Alternative names for Draxin antibody

C1orf187, PSEC0258, Neucrin, Dorsal repulsive axon guidance protein

SwissProt ID

B6ZI38 (Chick), P0C8S2 (Bovin), Q6PAL1 (Mouse), Q8NBI3 (Human)

Gene ID

374946 (C1orf187)

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), Por (Porcine)

Available hosts

Rabbit

Available applications

Dot blot (Dot), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of Draxin antibody

The secreted protein discovery initiative (SPDI) was established to identify novel secreted and trans-membrane proteins. The isolation of full-length cDNA clones for each of these candidate genes resulted in the identification of >1000 novel proteins. A total of 256 of these cDNAs are still novel, including variants and novel genes, per the most recent GenBank release version. The success of this large-scale effort was assessed by a bioinformatics analysis of the proteins through predictions of protein domains, subcellular localizations, and possible functional roles. The SPDI collection should facilitate efforts to better understand intercellular communication, may lead to new understandings of human diseases, and provides potential opportunities for the development of therapeutics. Chromosome 1 is gene-dense, with 3141 genes and 991 pseudogenes and many coding sequences overlap. The rearrangement and mutations in chromosome 1 are prevalent in cancer and many other diseases. Fine scale recombination occurs in hotspots of varying intensity along the sequences and these rearrangement and mutations are enriched near genes (1).
One of the gene on chromosome 1 codes for a secreted protein Draxin, that acts as chemorepulsive axon guidance molecule required for the development of spinal cord and forebrain commisures (2). There are several axonal guidance proteins including netrins, semaphorins, ephrins, slits and morphogens such as hedgehox, Wnts and bone morphogenetic proteins (BMPs) that are involved in development of nervous system. However, immense complexity of CNS makes it likely that there are several more factors that are yet to be identified. Using signal sequence trap screening, a recently identified dorsal repulsive axonal guidance protein, draxin from motoneurons, floorplate and roof plate of chick embryo is identified (3). Transient expression of draxin was noted during CNS and spinal cord development. In mouse draxin is expressed in several brain regions including cortex, olfactory bulb, mid-brain and pontine nuclei of the post natal day 0 mouse brain. Deduced amino acid sequence analyses of draxin suggest no homology with any characterized axonal guidance or morphogens. The chick draxin consists of amino terminal signal peptide sequence but no membrane anchoring motifs which suggest draxin is a secreted protein. Ectopic expression of draxin inhibits growth and disrupts the routing of commissural axons an is required for development of midline crossing forbrain commisures. It is suggested that draxoim may be involved in the formation of indusium grieseum glia (2).

General readings

Islam SM, Shinmyo Y, Okafuji T, et al. Draxin, a repulsive guidance protein for spinal cord and forebrain commissures. Science 2009; 323:388-93.

9 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Draxin (C-term) antibody

  Rabbit Polyclonal NEUCRIN Antibody  
Western blot analysis of Neucrin in rat cerebellum tissue lysate with Neucrin antibody at 1 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Draxin antibody

  Rabbit Polyclonal Anti-DRAXIN Antibody  
Host: Rabbit; Target Name: DRAXIN; Sample Tissue: 721_B Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, Hu, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

DRAXIN - human gene knockout kit via CRISPR

DRAXIN - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DRAXIN - human gene knockout kit via CRISPR

DRAXIN - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DRAXIN - human gene knockout kit via CRISPR

DRAXIN - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DRAXIN - human gene knockout kit via CRISPR

DRAXIN - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

DRAXIN overexpression lysate

DRAXIN overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DRAXIN (mGFP-tagged) - Human chromosome 1 open reading frame 187 (C1orf187), 200 uL, >10^7 TU/mL

Lenti ORF particles, DRAXIN (mGFP-tagged) - Human chromosome 1 open reading frame 187 (C1orf187), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, DRAXIN (Myc-DDK tagged) - Human chromosome 1 open reading frame 187 (C1orf187) , 200 uL, >10^7 TU/mL

Lenti ORF particles, DRAXIN (Myc-DDK tagged) - Human chromosome 1 open reading frame 187 (C1orf187) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

9 Item(s)

per page
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