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DTX3L / BBAP antibody

Principal name

DTX3L / BBAP antibody

Alternative names for DTX3L / BBAP antibody

Protein deltex-3-like, Rhysin-2, B-lymphoma- and BAL-associated protein

SwissProt ID

Q8TDB6 (Human)

Gene ID

151636 (DTX3L)

Ncbi ID

9606, NP_612144.1, NP_612144

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of DTX3L / BBAP antibody

Ubiquitin ligase that mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1), in response to DNA damage. Protects cells exposed to DNA-damaging agents. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). Involved in the recruitment of 53BP1/TP53BP1 to sites of DNA damage by mediating H4K91ub1 formation. In concert with PARP9, plays a role in PARP1-dependent DNA damage repair. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites.

General readings

Yan, Q., et al. Mol. Cell 36(1):110-120(2009).
Wilting, S.M., et al. Genes Chromosomes Cancer 47(10):890-905 (2008).
Juszczynski, P., et al. Mol. Cell. Biol. 26(14):5348-5359 (2006).
Takeyama, K., et al. J. Biol. Chem. 278(24):21930-21937 (2003).

Immunoperoxidase of monoclonal antibody to DTX3L on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 6 ug/ml]

H00151636-M01

DTX3L monoclonal antibody (M01), clone 1D10 Western Blot analysis of DTX3L expression in A-431 ( Cat # L015V1 ).

H00151636-M01

Detection limit for recombinant GST tagged DTX3L is approximately 0.3ng/ml as a capture antibody.

H00151636-M01

DTX3L polyclonal antibody (A01), Lot # ABNOVA060707QCS1 Western Blot analysis of DTX3L expression in A-431 ( Cat # L015V1 ).

H00151636-A01

Human lung tissue were subjected to SDS PAGE followed by western blot with 11963-1-AP(DTX3L antibody) at dilution of 1:500

11963-1-AP

Western Blot: DTX3L Antibody [NB100-2872] - Detection of Human DTX3L/BBAP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DTX3L/BBAP antibody NB100-2872 used for IP at 3 mcg/mg lysate. DTX3L/BBAP was also immunoprecipitated by another rabbit anti-DTX3L/BBAP antibody and NB100-2873, which recognize downstream epitopes. Detection: Chemiluminescence with an exposure time of 3 minutes.

NB100-2872

WB and Immunoprecipitation: DTX3L Antibody [NB100-2873] - Detection of Human and Mouse DTX3L/BBAP on HeLa whole cell lystate using NB100-2873. DTX3L/BBAP was also immunoprecipitated by another rabbit anti-DTX3L/BBAP antibody and NB100-2872.

NB100-2873

Western Blot: DTX3L Lysate [NBL1-10038] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for DTX3L

NBL1-10038

Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE GTX116369 diluted at 1:1000

GTX116369

Western blot analysis of DTX3L / BBAP Antibody Cat.-No AP51334PU-N in SK-BR-3 cell line lysates (35ug/lane). This demonstrates the DTX3L antibody detected the DTX3L protein (arrow).

AP51334PU-N

Immunohistochemistry-Paraffin: DTX3L Antibody [NBP1-88699] - Immunohistochemical staining of human lung shows strong cytoplasmic and membranous positivity in alveolar cells.

NBP1-88699

Western Blot: DTX3L Antibody [NBP1-88699] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.

NBP1-88699

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP51334PU-N	DTX3L / BBAP (Center) antibody
	Rabbit	Ig	Aff - Purified	Hu	WB	0.4 ml / €370.00
						Acris Antibodies GmbH

CRISPR

Catalog No.	Species	Donor Vector
KN207944	DTX3L - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,200.00
			OriGene Technologies, Inc.
KN207944BN	DTX3L - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN207944LP	DTX3L - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN207944RB	DTX3L - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP307944	DTX3L / BBAP
	Recombinant protein of human deltex 3-like (Drosophila) (DTX3L)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €680.00
				OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC207944L2V	Lenti ORF particles, DTX3L (mGFP-tagged) - Human deltex 3-like (Drosophila) (DTX3L), 200 uL, >10^7 TU/mL
		200 µl / €1,620.00
		OriGene Technologies, Inc.

RC207944L1V	Lenti ORF particles, DTX3L (Myc-DDK tagged) - Human deltex 3-like (Drosophila) (DTX3L) , 200 uL, >10^7 TU/mL
		200 µl / €1,460.00
		OriGene Technologies, Inc.

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