DUSP1 / MKP1 antibody

Principal name

DUSP1 / MKP1 antibody

Alternative names for DUSP1 / MKP1 antibody

CL100, MKP-1, PTPN10, VH1, MAP kinase phosphatase 1, Dual specificity protein phosphatase 1, Protein-tyrosine phosphatase CL100, Dual specificity protein phosphatase hVH1

SwissProt ID

P28562 (Human), P28563 (Mouse), Q64623 (Rat)

Gene ID

1843 (DUSP1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ze (Zebrafish), Rb (Rabbit), Sh (Sheep)

Available hosts

Goat, Mouse, Rabbit

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C)

Background of DUSP1 / MKP1 antibody

The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq]

General readings

1. Szelenyi ER & Urso ML Time-course analysis of injured skeletal muscle suggests a critical involvement of ERK1/2 signaling in the acute inflammatory response. Muscle Nerve 45:552-61 (2012). WB ; Mouse .
2. Asrih M  et al. Role of ERK1/2 activation in microtubule stabilization and glucose transport in cardiomyocytes. Am J Physiol Endocrinol Metab 301:E836-43 (2011). ICC/IF ; Rat . 

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DUSP1/4 antibody

Immunohistochemical analysis of DUSP1/4 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

DUSP1/4 antibody

Immunohistochemical analysis of DUSP1/4 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

DUSP1/4 antibody

Immunohistochemical analysis of DUSP1/4 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

DUSP1 / DUSP2 pSer296/318 antibody

Western blot analysis of extracts from K562 cells treated with Heatshock using Phospho-MKP-1/2 (Ser296/318) antibody AP55899PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

DUSP1 / DUSP2 pSer296/318 antibody

Western blot analysis of extracts from K562 cells treated with Heatshock using Phospho-MKP-1/2 (Ser296/318) antibody AP55899PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

DUSP1 / MKP1 (C-term) antibody

TA302731 staining (2µg/ml) of HeLa lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DUSP1 / MKP1 antibody

Western blot analysis of extracts from Jurkat cells, using MKP1 (Ab-359) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DUSP1 / MKP1 antibody

Western blot analysis of Mouse brain tissue using TA321654(DUSP1 Antibody) at dilution 1/363 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DUSP1 / MKP1 antibody

Western blot analysis of MKP-1/2 expression in Jurkat whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DUSP1 / MKP1 antibody

Western blot analysis of extracts of mouse fetal lung tissue lysate , using DUSP1 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DUSP1 / MKP1 (355-367) antibody

Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hst, Hu, L, Mky, Ms, Op, Platypus, Por, Prim, Rb, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DUSP1 / MKP1 (N-term) antibody

The anti-DUSP1 Pab is used in Western blot to detect DUSP1 in 293 cell lysate. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DUSP1 / MKP1 antibody

Immunohistochemistry analyzes of MKP1 antibody (AP20257PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DUSP1 / MKP1 (+MKP2) antibody

Immunohistochemistry (IHC) analyzes of MKP-1/2 antibody (Cat.-No.: AP21137PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

DUSP1 / MKP1 antibody

DUSP1 / MKP1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DUSP1 / MKP1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and DUSP1 HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DUSP1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 4H7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DUSP1 / MKP1 antibody

DUSP1 / MKP1 Mouse IgG2b 3A9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DUSP1 / MKP1 antibody

The DUSP1 polyclonal antibody ( Cat # PAB4162 ) is used in Western blot to detect DUSP1 in 293 cell lysate. Rabbit Hu E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

DUSP1 / MKP1 antibody

DUSP1 polyclonal antibody ( Cat # PAB6062 ) staining ( 2 µg/ml ) of HeLa lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DUSP1 / MKP1 pSer359 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using MKP1 (Phospho-Ser359) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, P
50 µg / €205.00
  Assay Biotech

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