DUSP10 / MKP5 antibody

Principal name

DUSP10 / MKP5 antibody

Alternative names for DUSP10 / MKP5 antibody

Dual specificity protein phosphatase 10, MAP kinase phosphatase 5, MKP-5, Mitogen-activated protein kinase phosphatase 5

SwissProt ID

Q0IID7 (Bovin), Q9ESS0 (Mouse), Q9Y6W6 (Human)

Gene ID

11221 (DUSP10)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot)

Background of DUSP10 / MKP5 antibody

Dual specificity protein phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the MAPK superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of this family of dual specificity phosphatases show distinct substrate specificities for MAPKs, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product binds to and inactivates p38 and SAPK/JNK, but not MAPK/ERK. Its subcellular localization is unique; it is evenly distributed in both the cytoplasm and the nucleus. This gene is widely expressed in various tissues and organs, and its expression is elevated by stress stimuli. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1, resulting in an isoform (b) that is shorter at the N-terminus compared to isoform a. Both variants 2 and 3 encode isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Tanoue T, Moriguchi T, Nishida E.
Molecular cloning and characterization of a novel dual specificity phosphatase, MKP-5.
J Biol Chem. 1999 Jul 9;274(28):19949-56.
PMID: 10391943

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DUSP10 / MKP5 (C-term) antibody

TA302470 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DUSP10 / MKP5 antibody

DUSP10 / MKP5 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DUSP10 / MKP5 antibody

WB Suggested Anti-DUSP10 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DUSP10 / MKP5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DUSP10 (RC206760, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUSP10. Mouse IgG2b OTI1F2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DUSP10 / MKP5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DUSP10 (RC206760, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUSP10. Mouse IgG2b OTI2E3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DUSP10 / MKP5 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DUSP10 (RC206760, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DUSP10.(1:2000) Mouse IgG2a OTI3D8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DUSP10 / MKP5 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-DUSP10 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA806771) Dilution: 1:150 Mouse IgG2a OTI2H1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

DUSP10 / MKP5 (N-term) antibody

(LEFT)Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. (RIGHT)Formalin-fixed and paraffin-embedded human testis tissue reacted with DUSP10 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DUSP10 / MKP5 antibody

DUSP10 polyclonal antibody (A01), Lot # 050928JC01 Western Blot analysis of DUSP10 expression in 293 ( Cat # L026V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DUSP10 / MKP5 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with DUSP10 polyclonal antibody ( Cat # PAB4136 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

DUSP10 / MKP5 antibody

DUSP10 polyclonal antibody ( Cat # PAB6116 ) staining ( 0.5 µg/ml ) of human liver lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DUSP10 / MKP5 antibody

Human THP-1; WB Suggested Anti-DUSP10 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; DUSP10 antibody - N-terminal region (ARP52762_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

DUSP10 / MKP5 antibody

NB 100-888 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

DUSP10 / MKP5 antibody

WB and IP: DUSP10 Antibody NBP1-30062 - Whole cell lysate from HeLa cells. Affinity purified NBP1-30062. DUSP10 was also IPed by NBP1-30062. For blotting NBP1-30062, the ReliaBLOT® Reagents and Procedures were used. Rabbit Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

DUSP10 / MKP5 antibody

Western Blot: DUSP10 Antibody [NBP1-31001] - Western blot analysis of DUSP10 Antibody on:  A: 293T 10% SDS PAGE Diluted at 1:500 Rabbit IgG Hu Dot, ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

DUSP10 / MKP5 antibody

Immunohistochemistry-Paraffin: DUSP10 Antibody [NBP1-82640] - Immunohistochemical staining of human kidney shows strong cytoplasmic positivity with granular pattern in renal tubules. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DUSP10 / MKP5 (149-482, His-tag)

DUSP10 / MKP5 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €940.00
  Acris Antibodies GmbH

DUSP10 / MKP5 (149-482, His-tag)

DUSP10 / MKP5 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €370.00
  Acris Antibodies GmbH

DUSP10 / MKP5

DUSP10 / MKP5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DUSP10 / MKP5

DUSP10 / MKP5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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