DUSP3 antibody

Principal name

DUSP3 antibody

Alternative names for DUSP3 antibody

VHR, Dual specificity protein phosphatase 3, Dual specificity protein phosphatase VHR, Vaccinia H1-related phosphatase

SwissProt ID

P51452 (Human), Q9D7X3 (Mouse)

Gene ID

1845 (DUSP3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of DUSP3 antibody

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene maps in a region that contains the BRCA1 locus which confers susceptibility to breast and ovarian cancer. Although DUSP3 is expressed in both breast and ovarian tissues, mutation screening in breast cancer pedigrees and in sporadic tumors was negative, leading to the conclusion that this gene is not BRCA1. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Alonso, A., Rahmouni, S., Williams, S., van Stipdonk, M., Jaroszewski, L., Godzik, A., Abraham, R.
T., Schoenberger, S. P., Mustelin, T. Tyrosine phosphorylation of VHR phosphatase by
ZAP-70. Nature Immun. 4: 44-48, 2003.
2. Ishibashi, T., Bottaro, D. P., Chan, A., Miki, T., Aaronson, S. A. Expression cloning of a human
dual-specificity phosphatase. Proc. Nat. Acad. Sci. 89: 12170-12174, 1992.
3. Patterson, K. I., Brummer, T., O'Brien, P. M., Daly, R. J. Dual-specificity phosphatases: critical
regulators with diverse cellular targets.Biochem. J. 418: 475-489, 2009.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DUSP3 antibody

Sample (50 ug of whole cell lysate). A: mouse stomach. 12% SDS PAGE. TA308662 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DUSP3 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DUSP3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: HeLa cell lysate 
Lane 2.: HepG2 cell lysate 
Lane 3.: Jurkat cell lysate 
Lane 4.: MCF7 cell lysate
Lane 5.: 293T cell lysate
Lane 6.: U87MG cell lysate
Lane 7.: K562 cell lysate Mouse IgG2b AT9E6 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

DUSP3 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DUSP3 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: HeLa cell lysate 
Lane 2.: HepG2 cell lysate 
Lane 3.: Jurkat cell lysate 
Lane 4.: MCF7 cell lysate
Lane 5.: 293T cell lysate
Lane 6.: U87MG cell lysate
Lane 7.: K562 cell lysate Mouse IgG2b AT9E6 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

DUSP3 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DUSP3 antibody

DUSP3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DUSP3 antibody

DUSP3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

DUSP3 antibody

Immunofluorescence of purified MaxPab antibody to DUSP3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

DUSP3 antibody

Western Blot analysis of DUSP3 expression in transfected 293T cell line by DUSP3 monoclonal antibody (M01), clone 5B7.

Lane 1: DUSP3 transfected lysate(20.478 KDa).
Lane 2: Non-transfected lysate. Mouse IgG2a 5B7 Purified Hu E, WB
50 µg / €370.00
  Abnova Taiwan Corp.

DUSP3 antibody

DUSP3 Mouse IgG2a 5B7 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

DUSP3 antibody

DUSP3 Mouse IgG1 2C4 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

DUSP3 antibody

Immunoblot analysis of cell lysates. Lane 1 : HepG2 cell lysate. Lane 2 : Hep3B cell lysate. Lane 3 : U87 mg cell lysate. Lane 4 : 293T cell lysate. Mouse IgG1 ARM-1 Hu IP, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

DUSP3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with DUSP3 polyclonal antibody ( Cat # PAB4127 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

DUSP3 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with DUSP3 polyclonal antibody ( Cat # PAB4175 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

DUSP3 antibody

DUSP3 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

DUSP3 antibody

Western Blot: DUSP3 Antibody [NBP1-33099] - Western blot analysis of DUSP3 on 293T lysates using NBP1-33099. Rabbit IgG Hu P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DUSP3

DUSP3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DUSP3 (1-185, His-tag)

DUSP3 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

DUSP3 (1-185, His-tag)

DUSP3 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

DUSP3

DUSP3 Human in vitro transl.
2 µg / €300.00
  Abnova Taiwan Corp.

DUSP3

DUSP3 Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

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