DUSP8 / VH5 antibody

Principal name

DUSP8 / VH5 antibody

Alternative names for DUSP8 / VH5 antibody

Dual specificity protein phosphatase 8

SwissProt ID

O09112 (Mouse), Q13202 (Human)

Gene ID

1850 (DUSP8)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat), Chk (Chicken), Can (Canine), GP (Guinea Pig)

Available hosts

Goat, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Dot blot (Dot)

Background of DUSP8 / VH5 antibody

DUSP8 is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. DUSP8inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin.The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates SAPK/JNK and p38, is expressed predominantly in the adult brain, heart, and skeletal muscle, is localized in the cytoplasm, and is induced by nerve growth factor and insulin. An intronless pseudogene for DUSP8 is present on chromosome 10q11.2.

General readings

Martell KJ, Seasholtz AF, Kwak SP, Clemens KK, Dixon JE.
hVH-5: a protein tyrosine phosphatase abundant in brain that inactivates mitogen-activated protein kinase.
J Neurochem. 1995 Oct;65(4):1823-33.
PMID: 7561881

19 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DUSP8 / VH5 (N-term) antibody

TA302764 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DUSP8 / VH5 antibody

Sample(30 ug of whole cell lysate). A:Raji . 7.5% SDS PAGE. TA308797 diluted at 1:1000 Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DUSP8 / VH5 antibody

Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using DUSP8 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DUSP8 / VH5 antibody

Gel: 6%SDS-PAGE, Lysate: 40 µg, Lane: 293T cells, Primary antibody: DUSP8 Antibody at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DUSP8 / VH5 antibody

WB Suggested Anti-DUSP8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 2500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DUSP8 / VH5 (250-300) antibody

Heart: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

DUSP8 / VH5 (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. Rabbit Ig Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

DUSP8 / VH5 antibody

Western blot analysis of DUSP8 in HeLa cell lysate. Using DUSP8 polyclonal antibody ( Cat # PAB0347 ) at 2 µg/ml. Rabbit Hu WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DUSP8 / VH5 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with DUSP8 polyclonal antibody ( Cat # PAB4135 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

DUSP8 / VH5 antibody

DUSP8 polyclonal antibody ( Cat # PAB6114 ) staining ( 2 µg/ml ) of human heart lysate ( RIPA buffer, 30 µg total protein per lane ) . Primary incubated for 12 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

DUSP8 / VH5 antibody

Transfected 293T; WB Suggested Anti-DUSP8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: Transfected 293T; DUSP8 antibody - middle region (ARP52051_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

DUSP8 / VH5 antibody

NB 100-886 staining (2µg/ml) of Human Heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

DUSP8 / VH5 antibody

Western Blot: DUSP8 Antibody [NB100-56410] - Western blot analysis of DUSP8 in HeLa cell lysate using NB100-56410 at 2 ug/ml. Rabbit IgG Purified Hu WB
0.1 mg / €320.00
  Novus Biologicals Inc.

DUSP8 / VH5 antibody

Western Blot: DUSP8 Antibody [NBP1-31169] - Western blot analysis of DUSP8 Antibody on: A:Raji 7.5% SDS PAGE diluted at 1:1000 Rabbit IgG Hu Dot, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

DUSP8 / VH5 antibody

Western Blot: DUSP8 Antibody [NBP1-58302] - This Anti-DUSP8 antibody was used in Western Blot of Transfected 293T   tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

DUSP8 / VH5 antibody

Immunohistochemistry-Paraffin: DUSP8 Antibody [NBP1-88385] Immunohistochemical staining of human cerebral cortex shows strong positivity in glial cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DUSP8 / VH5

DUSP8 / VH5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DUSP8 / VH5

DUSP8 / VH5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DUSP8 overexpression lysate

DUSP8 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

19 Item(s)

per page
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