DUSP9 / MKP4 antibody
Principal name
DUSP9 / MKP4 antibody
Alternative names for DUSP9 / MKP4 antibody
MAP kinase phosphatase 4, Dual specificity protein phosphatase 9, Mitogen-activated protein kinase phosphatase 4
SwissProt ID
Q99956 (Human)
Gene ID
1852 (DUSP9)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Dot blot (Dot)
Background of DUSP9 / MKP4 antibody
Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways that are activated by a range of stimuli and mediate a number of physiological and pathological changes in the cell. Dual specificity phosphatases (DSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. The members of the dual-specificity phosphatase protein family include MKP-1/CL100 (3CH134), VHR, PAC1, MKP-2, hVH-3 (B23), hVH-5, MKP-3, MKP-X and MKP-4. Human MKP-1 maps to chromosome 5q34 and encodes a 367 amino acid, 39 kDa protein that dephosphorylates MAP kinase ERK2 on both Thr-183 and Tyr-185.
Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and DUSP9. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DUSP9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.
DUSP9 monoclonal antibody (M04), clone 2E3 Western Blot analysis of DUSP9 expression in HeLa ( Cat # L013V1 ).
Western Blot analysis of DUSP9 expression in transfected 293T cell line (H00001852-T03) by DUSP9 MaxPab polyclonal antibody.Lane 1: DUSP9 transfected lysate(41.90 KDa).Lane 2: Non-transfected lysate.
Western Blot: DUSP9 Lysate [NBL1-10059] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for DUSP9
Immunohistochemistry: MKP-4 Antibody [NBP1-19611] - MKP-4 Antibody [NBP1-19611] - paraffin-embedded human liver carcinoma tissue.
Western Blot: MKP-4 Antibody [NBP1-19611] - extracts from HeLa cells.
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using MKP-4 (GTX109288) antibody at 1:100 dilution.
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MKP-4 (GTX109288) antibody at 1:200 dilution.
Sample(30 ug whole cell lysate)A:A431(GTX27909)10% SDS PAGEGTX109288 diluted at 1:1000
Immunoblot analysis of HepG2 cell lysates with DUSP9 monoclonal antibody, clone AF11B5.
Immunohistochemical of paraffin-embedded human kidney using 14484-1-AP(DUSP9 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human kidney using 14484-1-AP(DUSP9 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using DUSP9 antibody 14484-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
HeLa cells were subjected to SDS PAGE followed by western blot with 14484-1-AP(DUSP9 antibody) at dilution of 1:500
Human liver tissue were subjected to SDS PAGE followed by western blot with 14484-1-AP(DUSP9 antibody) at dilution of 1:300
Human kidney tissue were subjected to SDS PAGE followed by western blot with 14484-1-AP(DUSP9 antibody) at dilution of 1:500
Immunofluorescence: DUSP9 Antibody [NBP1-31227] - Immunofluorescence analysis of A431, using DUSP9 antibody at 1:200 dilution.
Western Blot: DUSP9 Antibody [NBP1-31227] - Western blot analysis of DUSP9 Antibody on: A:A431 10% SDS PAGE diluted at 1:1000
Immunohistochemistry (IHC) analyzes of MKP-4 antibody (Cat.-No.: AP20456PU-N) in paraffin-embedded human liver carcinoma tissue.
Western blot (WB) analysis of MKP-4 antibody (Cat.-No.: AP20456PU-N) in extracts from HeLa cells.
Human Fetal Brain; WB Suggested Anti-DUSP9 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Brain; DUSP9 antibody - middle region (ARP61993_P050) in Human Fetal Brain cells using Western Blot
Immunohistochemistry-Paraffin: DUSP9 Antibody [NBP1-82641] - Immunohistochemical staining of human placenta shows moderate cytoplasmic and nuclear positivity in trophoblastic cells.
Immunofluorescence: DUSP9 Antibody [NBP1-82641] - 2 OS shows positivity in endoplasmic reticulum.
Western Blot: DUSP9 Antibody [NBP1-82641] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: Negative control (vector only transfected HEK293T lysate) | Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Immunofluorescence analysis of HeLa cells, using DUSP9 Antibody Cat.-No AP32268PU-N. The picture on the right is treated with the synthesized peptide.
Western blot analysis of extracts from HeLa cells, using DUSP9 Antibody Cat.-No AP32268PU-N. The lane on the right is treated with the synthesized peptide.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA313774 | DUSP9 / MKP4 antibody |
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Rabbit | IgG | Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 ml /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
 +2 additional images |
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| AP20456PU-N | DUSP9 / MKP4 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€350.00
|
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| Acris Antibodies GmbH | |||||||
| +1 additional image |
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| AP32268PU-N | DUSP9 / MKP4 antibody |
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 |
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
50 µg /
€470.00
|
|
| Acris Antibodies GmbH | |||||||
| +2 additional images |
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| H00001852-A01 | DUSP9 / MKP4 antibody |
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 |
Mouse | Serum | Hu | E, WB |
50 µl /
€290.00
|
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| Abnova Taiwan Corp. | |||||||
| H00001852-D01P | DUSP9 / MKP4 antibody |
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 |
Rabbit | Purified | Hu | E(detection), WB |
0.1 mg /
€380.00
|
||
| Abnova Taiwan Corp. | |||||||
| H00001852-M04 | DUSP9 / MKP4 antibody |
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 |
Mouse | IgG2a | 2E3 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| +1 additional image |
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| MAB5559 | DUSP9 / MKP4 antibody |
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 |
Mouse | IgG1 | AF11B5 | Hu | E, WB |
0.1 ml /
€390.00
|
|
| Abnova Taiwan Corp. | |||||||
| C11586-1 | DUSP9 / MKP4 antibody |
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 |
Rabbit | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
50 µg /
€205.00
|
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| Assay Biotech | |||||||
| +2 additional images |
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| C11586-2 | DUSP9 / MKP4 antibody |
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|
Rabbit | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
€310.00
|
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| Assay Biotech | |||||||
| +2 additional images |
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| ARP61993_P050 | DUSP9 / MKP4 (Center) antibody |
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 |
Rabbit | Aff - Purified | Hu | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| NBP1-19611 | DUSP9 / MKP4 antibody |
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![Immunohistochemistry: MKP-4 Antibody [NBP1-19611] - MKP-4 Antibody [NBP1-19611] - paraffin-embedded human liver carcinoma tissue.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/NBP1-19611_2.jpg) |
Rabbit | Aff - Purified | Hu, Ms, Rt | ICC/IF, P, WB |
0.1 mg /
€420.00
|
||
| Novus Biologicals Inc. | |||||||
| +1 additional image |
|||||||
| NBP1-31227 | DUSP9 / MKP4 antibody |
||||||
![Immunofluorescence: DUSP9 Antibody [NBP1-31227] - Immunofluorescence analysis of A431, using DUSP9 antibody at 1:200 dilution.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/109677_2.jpg) |
Rabbit | IgG | Hu | Dot, ICC/IF, P, WB |
0.1 mg /
€420.00
|
||
| Novus Biologicals Inc. | |||||||
| +1 additional image |
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| NBP1-68136-0.1 | DUSP9 / MKP4 antibody |
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|
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
0.1 mg /
€360.00
|
|
| Novus Biologicals Inc. | |||||||
| NBP1-68136-50 | DUSP9 / MKP4 antibody |
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|
Rabbit | IgG | Aff - Purified | Hu, Ms, Rt | E, ICC/IF, P, WB |
50 µg /
€290.00
|
|
| Novus Biologicals Inc. | |||||||
| NBP1-82641 | DUSP9 / MKP4 antibody |
||||||
![Immunohistochemistry-Paraffin: DUSP9 Antibody [NBP1-82641] - Immunohistochemical staining of human placenta shows moderate cytoplasmic and nuclear positivity in trophoblastic cells.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/DUSP9 Antibody-Immunohistochemistry-Paraffin-NBP1-82641-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | ICC/IF, P, WB |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
| +2 additional images |
|||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309271 | DUSP9 / MKP4 |
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 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
Regular Price: 20 µg / €680.00 Special Price: 20 µg / €398.00 |
|
| OriGene Technologies, Inc. | |||||
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| H00001852-P01-10 | DUSP9 / MKP4 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00001852-P01-25 | DUSP9 / MKP4 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
| H00001852-Q01-10 | DUSP9 / MKP4 |
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|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00001852-Q01-25 | DUSP9 / MKP4 |
||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
