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DUSP9 / MKP4 antibody

Principal name

DUSP9 / MKP4 antibody

Alternative names for DUSP9 / MKP4 antibody

MAP kinase phosphatase 4, Dual specificity protein phosphatase 9, Mitogen-activated protein kinase phosphatase 4

SwissProt ID

Q99956 (Human)

Gene ID

1852 (DUSP9)

Ncbi ID

9606, NP_001386, NP_001386.1, NM_001395

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of DUSP9 / MKP4 antibody

Mitogen-activated protein (MAP) kinases are a large class of proteins involved in signal transduction pathways that are activated by a range of stimuli and mediate a number of physiological and pathological changes in the cell. Dual specificity phosphatases (DSPs) are a subclass of the protein tyrosine phosphatase (PTP) gene superfamily, which are selective for dephosphorylating critical phosphothreonine and phosphotyrosine residues within MAP kinases. DSP gene expression is induced by a host of growth factors and/or cellular stresses, thereby negatively regulating MAP kinase superfamily members including MAPK/ERK, SAPK/JNK and p38. The members of the dual-specificity phosphatase protein family include MKP-1/CL100 (3CH134), VHR, PAC1, MKP-2, hVH-3 (B23), hVH-5, MKP-3, MKP-X and MKP-4. Human MKP-1 maps to chromosome 5q34 and encodes a 367 amino acid, 39 kDa protein that dephosphorylates MAP kinase ERK2 on both Thr-183 and Tyr-185.

Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and DUSP9. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-DUSP9 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.

H00001852-M04

DUSP9 monoclonal antibody (M04), clone 2E3 Western Blot analysis of DUSP9 expression in HeLa ( Cat # L013V1 ).

H00001852-M04

Western Blot analysis of DUSP9 expression in transfected 293T cell line (H00001852-T03) by DUSP9 MaxPab polyclonal antibody.Lane 1: DUSP9 transfected lysate(41.90 KDa).Lane 2: Non-transfected lysate.

H00001852-D01P

Immunoblot analysis of HepG2 cell lysates with DUSP9 monoclonal antibody, clone AF11B5.

MAB5559

Immunohistochemistry (IHC) analyzes of MKP-4 antibody (Cat.-No.: AP20456PU-N) in paraffin-embedded human liver carcinoma tissue.

AP20456PU-N

Western blot (WB) analysis of MKP-4 antibody (Cat.-No.: AP20456PU-N) in extracts from HeLa cells.

AP20456PU-N

Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DUSP9 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using DUSP9 Antibody. The picture on the right is treated with the synthesized peptide.

Western blot analysis of extracts from HeLa cells, using DUSP9 Antibody. The lane on the right is treated with the synthesized peptide.

Human Fetal Brain; WB Suggested Anti-DUSP9 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Brain; DUSP9 antibody - middle region (ARP61993_P050) in Human Fetal Brain cells using Western Blot

ARP61993_P050

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

AP32268PU-N

Immunofluorescence analysis of HeLa cells, using DUSP9 Antibody Cat.-No AP32268PU-N. The picture on the right is treated with the synthesized peptide.

AP32268PU-N

Western blot analysis of extracts from HeLa cells, using DUSP9 Antibody Cat.-No AP32268PU-N. The lane on the right is treated with the synthesized peptide.

AP32268PU-N

TP309271

Primary Antibodies

Catalog No.	Host	Iso.	Pres.	React.	Applications
AP20456PU-N	DUSP9 / MKP4 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	ICC/IF, P, WB	0.1 mg / €350.00
						Acris Antibodies GmbH
+1 additional image
AP32268PU-N	DUSP9 / MKP4 antibody
	Rabbit	IgG	Aff - Purified	Hu, Ms, Rt	E, ICC/IF, P, WB	50 µl / €380.00
						Acris Antibodies GmbH
+2 additional images
C11586-1	DUSP9 / MKP4 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	E, ICC/IF, P, WB	50 µg / €230.00
						Assay Biotech
+2 additional images
C11586-2	DUSP9 / MKP4 antibody
	Rabbit		Aff - Purified	Hu, Ms, Rt	E, ICC/IF, P, WB	0.1 mg / €330.00
						Assay Biotech
+2 additional images

CRISPR

Catalog No.	Species	Donor Vector
KN209271	DUSP9 - human gene knockout kit via CRISPR
	Human	GFP-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN209271BN	DUSP9 - human gene knockout kit via CRISPR
	Human	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN209271LP	DUSP9 - human gene knockout kit via CRISPR
	Human	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN209271RB	DUSP9 - human gene knockout kit via CRISPR
	Human	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No.	Species	Purity	Source
TP309271	DUSP9 / MKP4
	Recombinant protein of human dual specificity phosphatase 9 (DUSP9)
	Human	> 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.	HEK293 cells	20 µg / €438.00
				OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC209271L2V	Lenti ORF particles, DUSP9 (mGFP-tagged) - Human dual specificity phosphatase 9 (DUSP9), 200 uL, >10^7 TU/mL
		200 µl / €1,082.00
		OriGene Technologies, Inc.

RC209271L1V	Lenti ORF particles, DUSP9 (Myc-DDK tagged) - Human dual specificity phosphatase 9 (DUSP9) , 200 uL, >10^7 TU/mL
		200 µl / €969.00
		OriGene Technologies, Inc.

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