DUT antibody

Principal name

DUT antibody

Alternative names for DUT antibody

dUTPase, dUTP pyrophosphatase, Deoxyuridine 5'-triphosphate nucleotidohydrolase mitochondrial

SwissProt ID

P33316 (Human), P33317 (Yeast), P70583 (Rat)

Gene ID

1854 (DUT), 497778 (Dut)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Chicken, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunoprecipitation (IP)

Background of DUT antibody

DUT is an essential enzyme of nucleotide metabolism. This protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19.

General readings

Studebaker,A.W., (2005) Biochem. Biophys. Res. Commun. 327 (1), 306-310

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DUT antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1C9 Hu E, ICC/IF, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

DUT antibody

  Anti-DUT Rabbit Polyclonal Antibody  
Predicted band size: 27 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DUT antibody

  Anti-DUT Rabbit Polyclonal Antibody  
Predicted band size: 27 kDa. Positive control: 293T cell lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

DUT antibody

  Rabbit Polyclonal Anti-DUT Antibody  
WB Suggested Antibody Titration: 2.5 ug/ml; Positive Control: Jurkat.DUT is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye, Ze P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DUT antibody

  Rabbit Polyclonal Anti-DUT Antibody  
WB Suggested Anti-DUT Antibody Titration: 1 ug/ml; Positive Control: Fetal Small Intestine cell lysate Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

DUT (1-52) antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Chicken Ig Azide Free Hu P, WB
50 µg / €530.00
  Acris Antibodies GmbH

DUT antibody

Immunohistochemical analysis of paraffin embedded rat tissue sections (cardiac muscle), staining DUTPase in cytoplasm, DAB chromogenic reaction Rabbit IgG Aff - Purified Hu, Ms, Rt IP, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

DUT antibody

AP33499PU-N (0.1µg/ml) staining of HEK293 (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence Goat Aff - Purified Bov, Hu, Por WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

DUT - human gene knockout kit via CRISPR

DUT - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DUT - human gene knockout kit via CRISPR

DUT - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DUT - human gene knockout kit via CRISPR

DUT - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DUT - human gene knockout kit via CRISPR

DUT - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DUT (transcript variant 3)

  Purified recombinant protein of Homo sapiens deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, transcript variant 3  
DUT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DUT (transcript variant 2)

  Recombinant protein of human deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, transcript variant 2  
DUT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

DUT (70-252, His-tag)

DUT Human Purified > 90 % by SDS – PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

DUT (70-252, His-tag)

DUT Human Purified > 90 % by SDS – PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, DUT (mGFP-tagged)-Human deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, DUT (mGFP-tagged)-Human deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, DUT (mGFP-tagged) - Human deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, DUT (mGFP-tagged) - Human deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, DUT (Myc-DDK-tagged)-Human deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, DUT (Myc-DDK-tagged)-Human deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Lenti ORF particles, DUT (Myc-DDK tagged) - Human deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, transcript variant 3 , 200 uL, >10^7 TU/mL

Lenti ORF particles, DUT (Myc-DDK tagged) - Human deoxyuridine triphosphatase (DUT), nuclear gene encoding mitochondrial protein, transcript variant 3 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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