DYDC1 antibody

Principal name

DYDC1 antibody

Alternative names for DYDC1 antibody

DPY30D1, RSD9, RSD-9, LOC143241, DPY30 domain-containing protein 1

SwissProt ID

Q32LH1 (Bovin), Q8WWB3 (Human), Q9D9T0 (Mouse)

Gene ID

143241 (DYDC1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of DYDC1 antibody

The Dumpy-30 (DPY-30) protein was first described in C. elegans, in which it is involved in dosage compensation of sex chromosomes. Conserved from yeast to humans, the DPY-30 family is involved in gene expression and chromatin modification, specifically histone methylation. DPY-30 and closely related proteins contain a short motif that is related to the dimerization motif in the regulatory subunit of protein kinase A (PKA), which consists of two ?helices that form a four-helix bundle during dimerization. As a member of the DPY-30 family, DYDC1 (DPY30 domain-containing protein 1), also known as DPY30D1 and RSD9, is a 177 amino acid protein that binds to Endophilin III and plays a crucial role during acrosome biogenesis. DYDC1 is specifically expressed in brain and testis and accumulates in the acrosome area during spermatogenesis. Knockdown of DYDC1 mRNA results in disruption of acrosome formation and spermatid differentiation.

General readings

1. Deloukas P, Earthrowl ME, Grafham DV, Rubenfield M, French L, Steward CA, Sims SK, Jones MC, Searle S, Scott C, Howe K, Hunt SE, Andrews TD, Gilbert JG, Swarbreck D, Ashurst JL, Taylor A, Battles J, Bird CP, Ainscough R, Almeida JP, Ashwell RI, Ambrose KD. The DNA sequence and comparative analysis of human chromosome 10.
Nature 2004 May 429 (6990): 375-81.
PMID: 15164054

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DYDC1 antibody

Lane A: AP23775PU-N 1µg/ml) staining of HEK293 overexpressing Human DYDC1 lysate (10µg protein in RIPA buffer).
Lane B: AP23775PU-N (1µg/ml) staining of HEK293 mock-transfected lysate (10µg protein in RIPA buffer). 
Lane C: anti-MYC Tag (1/1000) staining HEK293 overexpressing Human DYDC1 lysate (10µg protein in RIPA buffer). 
Primary incubations were for 1 hour. 
Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

DYDC1 (142-154) antibody

Lane A - TA311571 (1ug/ml) staining of HEK293 overexpressing Human DYDC1 lysate (10ug protein in RIPA buffer). Lane B - TA311571 (0.1ug/ml) staining of HEK293 mock-transfected lysate (10ug protein in RIPA buffer). Lane C - anti-MYC Tag (1:1000) staining HEK293 overexpressing Human DYDC1 lysate (10ug protein in RIPA buffer). Primary incubations were for 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

DYDC1 antibody

WB Suggested Anti-DYDC1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: PANC1 cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

DYDC1 antibody

DYDC1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

DYDC1 antibody

Immunofluorescence of monoclonal antibody to DYDC1 on HeLa cell . [antibody concentration 10 ug/ml] Mouse IgG2b 1B11 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DYDC1 antibody

DYDC1 Mouse IgG1 2B8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DYDC1 antibody

Human PANC1; WB Suggested Anti-DYDC1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: PANC1 cell lysate; DYDC1 antibody - middle region (ARP52659_P050) in Human PANC1 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

DYDC1 antibody

DYDC1 Goat Aff - Purified Hu E, WB
0.1 mg / €320.00
  Novus Biologicals Inc.

DYDC1 antibody

DYDC1 Goat Aff - Purified Bov, Hu, Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

DYDC1 antibody

Immunohistochemistry-Paraffin: DYDC1 Antibody [NBP1-88779] - Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

DYDC1 antibody

Immunohistochemistry-Paraffin: DYDC1 Antibody [NBP1-88780] - Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DYDC1

DYDC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DYDC1

DYDC1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

DYDC1

DYDC1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

DYDC1 overexpression lysate

DYDC1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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