Dynamin-1 antibody

Principal name

Dynamin-1 antibody

Alternative names for Dynamin-1 antibody

DNM1, DNM

SwissProt ID

P21575 (Rat), P39053 (Mouse), Q05193 (Human), Q08DF4 (Bovin), Q61359 (Mouse)

Gene ID

1759 (DNM1), 140694 (Dnm1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Can (Canine), Mky (Monkey)

Available hosts

Rabbit, Mouse, Sheep

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Frozen Sections (C)

Background of Dynamin-1 antibody

Dynamin is a GTPase required for endocytosis of clathrin coated vesicles from the membrane. Dynamin's involvement in endocytosis was first discovered during the characterization of the temperature sensitive parylytic shibire mutation from Drosophila melanogaster. Three closely related isoforms have been identified in mammals. Dynamin1 is expressed exclusively in neurons, dynamin2 is ubiquitously expressed and dynamin3 is expressed primarily in the testes. Each of these isoforms of dynamin also has several splice variants. This suggests that each isoform/splice variant may have separate intracellular targets or function in the budding of distinct vesicle types. Common domain features of the dynamin proteins include an amino acid terminal GTPase domain, a central pleckstrin homology domain and a highly basic proline rich carboxyl terminus with several Src homology 3 (SH3) domains. These SH3 domains target the dynamin to the clathrin coated pit. Dynamin is known to interact with at least two proteins, AP2 adaptor protein complex and amphiphysin.

General readings

1. Mori H  et al. Transmembrane/cytoplasmic, rather than catalytic, domains of Mmp14 signal to MAPK activation and mammary branching morphogenesis via binding to integrin ß1. Development 140:343-52 (2013). WB ; Mouse .
2. Martins-de-Souza D  et al. Phosphoproteomic differences in major depressive disorder postmortem brains indicate effects on synaptic function. Eur Arch Psychiatry Clin Neurosci 262:657-66 (2012). WB ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Dynamin-1 pSer774 antibody

Figure 1 Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Ser774 Dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Sheep IgG Aff - Purified Bov, Can, Chk, Hu, Ms, non-Human Primates, Rt C, ICC/IF, WB
0.1 ml / €380.00
  Acris Antibodies GmbH

Dynamin-1 (N-term) antibody

Western Blot of rat cortex lysate showing labeling of  the ~95k Dynamin protein. Rabbit IgG Aff - Purified Bov, Chk, Hu, Ms, non-Human Primates, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Dynamin-1 antibody

Western blot analysis of extracts from HeLa cells, using DYN1 (Ab-4520) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Dynamin-1 antibody

Gel: 8%SDS-PAGE, Lysate: 30 µg, Lane: Mouse brain tissue, Primary antibody: DNM1 Antibody at dilution 1/100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Dynamin-1 antibody

Gel: 8%SDS-PAGE, Lysate: 40 µg, Lane: Mouse brain tissue, Primary antibody: DNM1 Antibody at dilution 1/700, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Dynamin-1 antibody

Western blot analysis of Dynamin-1 expression in Mouse braintissue lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Dynamin-1 antibody

Western Blot: Dynamin Antibody (3G4B6) [TA336537] - Western blot analysis using anti-Dynamin monoclonal antibody against truncated Dynamin recombinant protein (1), SK-N-SH (2) and NIH3T3 (3) cell lysate. Mouse IgG2a 3G4B6 Purified Hu C, E, F, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Dynamin-1 antibody

Western blot analysis of extracts from Hela cells using Dynamin 1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Dynamin-1 antibody

Immunohistochemical analysis of paraffin-embedded human lymph tissue (A), glioma tissue (B) and cerebellum tissue (C), showing membrane localization using Dynamin-1 antibody Cat.-No AM06123SU-N with DAB staining Mouse IgG2a 3G4B6 Ascites Hu P, WB
0.1 ml / €480.00
  Acris Antibodies GmbH

Dynamin-1 pSer774 antibody

Dynamin-1 Sheep IgG Purified Bov, Can, Chk, Hu, Mky, Ms, Rt C, ICC/IF, WB
0.1 ml / €500.00
  Acris Antibodies GmbH

Dynamin-1 pSer778 antibody

Dynamin-1 Sheep IgG Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 ml / €510.00
  Acris Antibodies GmbH

Dynamin-1 antibody

Immunohistochemistry analyzes of Dynamin-1 antibody (Cat.-No.: AP06385PU-N) in Paraffin-Embedded Human tbrain tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Dynamin-1 (C-term) antibody

AP08309PU-N Dynamin-1 antibody staining of Formalin-Fixed Paraffin Embedded Human Brain, Cortex at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit IgG Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Dynamin-1 pSer778 antibody

Figure 1 Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser778 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser778 Dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase. Sheep IgG Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 ml / €380.00
  Acris Antibodies GmbH

Dynamin-1 antibody

Dynamin-1 Rabbit Hu E, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Dynamin-1 antibody

Western blot of rat cortex lysate showing specific immunolabeling of the ~95k Dynamin protein. Using Dynamin polyclonal antibody ( Cat # PAB9596 ). Rabbit IgG Bov, Chk, Hu, Ms, Rt C
0.1 ml / €470.00
  Abnova Taiwan Corp.

Dynamin-1 pSer774 antibody

Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Dynamin (phospho S774) polyclonal antibody ( Cat # PAB9697 ). The immunolabeling is completely eliminated by treatment with l-Ptase. Sheep IgG Bov, Chk, Hu, Ms, Rt C, ICC/IF
0.1 ml / €510.00
  Abnova Taiwan Corp.

Dynamin-1 pSer778 antibody

Western blot of rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k Dynamin phosphorylated at Ser778 (Control, lane 1). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: l-Ptase, lane 2). The blot is identical to the control except that it was incubated in l-Ptase (1200 units for 30 min) before being exposed to the Dynamin (phospho S778) polyclonal antibody ( Cat # PAB9698 ). The immunolabeling is completely eliminated by treatment with l-Ptase. Sheep IgG Bov, Chk, Hu, Ms, Rt
0.1 ml / €510.00
  Abnova Taiwan Corp.

Dynamin-1 pSer774 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Dynamin-1 (Phospho-Ser774) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

Dynamin-1 pSer774 antibody

Immunohistochemistry analysis of paraffin-embedded human brain, using Dynamin-1 (Phospho-Ser774) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

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