DYNLL1 antibody

Principal name

DYNLL1 antibody

Alternative names for DYNLL1 antibody

Dynein light chain 1, DLC1, DNCL1, DNCLC1, HDLC1, DLC8, PIN, Dynein light chain 1 cytoplasmic, 8 kDa dynein light chain, Dynein light chain LC8-type 1, Protein inhibitor of neuronal nitric oxide synthase

SwissProt ID

P61285 (Bovin), P63167 (Human), P63168 (Mouse), P63169 (Rabit), P63170 (Rat), Q02647 (Yeast), Q22799 (Caeel), Q24117 (Drome), Q9UR05 (Schpo)

Gene ID

8655 (DYNLL1), 56455 (Dynll1), 58945

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Rb (Rabbit), Rt (Rat), African clawed frog, Dros (Drosophila), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine)

Available hosts

Mouse, Rat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of DYNLL1 antibody

Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. DYNLL1 is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity.Cytoplasmic dyneins are large enzyme complexes with a molecular mass of about 1,200 kD. They contain two force-producing heads formed primarily from dynein heavy chains, and stalks linking the heads to a basal domain, which contains a varying number of accessory intermediate chains. The complex is involved in intracellular transport and motility. The protein described in this record is a light chain and exists as part of this complex but also physically interacts with and inhibits the activity of neuronal nitric oxide synthase. Binding of this protein destabilizes the neuronal nitric oxide synthase dimer, a conformation necessary for activity, and it may regulate numerous biologic processes through its effects on nitric oxide synthase activity. Alternate transcriptional splice variants have been characterized.

General readings

Rapali P., et al. (2011) FEBS J. 278(17): 2980-2996.Radnai L., et al. (2010) J Biol Chem. 285(49): 38649-38657.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

DYNLL1 antibody

  Rabbit anti-DYNLL1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using DYNLL1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

DYNLL1 antibody

  Rabbit Polyclonal Anti-DYNLL1  
WB Suggested Anti-DYNLL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DYNLL1 antibody

  Rabbit Polyclonal Anti-DYNLL1  
WB Suggested Anti-DYNLL1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Transfected 293T Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

DYNLL1 antibody

  Rabbit Polyclonal Anti-DYNLL1 Antibody  
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane: Mouse brain tissue, Primary antibody: (DYNLL1 Antibody) at dilution 1/600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

DYNLL1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DYNLL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : A549 cell lysate
Lane 3. : 293T cell lysate
Lane 4. : HepG2 cell lysate Mouse IgG2b AT13C9 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

DYNLL1 antibody

The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human DYNLL antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1. : HeLa cell lysate 
Lane 2. : A549 cell lysate
Lane 3. : 293T cell lysate
Lane 4. : HepG2 cell lysate Mouse IgG2b AT13C9 Purified Hu E, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

DYNLL1 antibody

DYNLL1 Mouse IgG2a 1H7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

DYNLL1 antibody

DYNLL1 Rat IgM 10D6 Hu, Ms F, WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

DYNLL1 (+ DYNLL2) antibody

DYNLL1 Rat IgG2a 11F7 Hu, Ms F, IP, WB
0.1 mg / €820.00
  Abnova Taiwan Corp.

DYNLL1 antibody

Transfected 293T; WB Suggested Anti-DYNLL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Transfected 293T; DYNLL1 antibody - middle region (ARP51261_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rb, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

DYNLL1 antibody

Transfected 293T; WB Suggested Anti-DYNLL1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Transfected 293T; DYNLL1 antibody - N-terminal region (ARP51350_P050) in Transfected 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Dros, Hu, Ms, Rb, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

CRISPR

Catalog No. Species Donor Vector  

DYNLL1 - human gene knockout kit via CRISPR

DYNLL1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

DYNLL1 - human gene knockout kit via CRISPR

DYNLL1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

DYNLL1 - human gene knockout kit via CRISPR

DYNLL1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

DYNLL1 - human gene knockout kit via CRISPR

DYNLL1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

DYNLL1 (transcript variant 2)

  Recombinant protein of human dynein, light chain, LC8-type 1 (DYNLL1), transcript variant 2  
DYNLL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DYNLL1 (transcript variant 3)

  Recombinant protein of human dynein, light chain, LC8-type 1 (DYNLL1), transcript variant 3  
DYNLL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DYNLL1 (transcript variant 1)

  Recombinant protein of human dynein, light chain, LC8-type 1 (DYNLL1), transcript variant 1  
DYNLL1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

DYNLL1 (transcript variant 1)

  Recombinant protein of human dynein, light chain, LC8-type 1 (DYNLL1), transcript variant 1  
DYNLL1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €399.00
  OriGene Technologies, Inc.

DYNLL1 (1-89, His-tag)

DYNLL1 Human Purified > 90 % E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

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