Dysadherin / FXYD5 antibody

Principal name

Dysadherin / FXYD5 antibody

Alternative names for Dysadherin / FXYD5 antibody

DYSAD, IWU1, FXYD domain-containing ion transport regulator 5

SwissProt ID

P59647 (Rat), P97808 (Mouse), Q96DB9 (Human)

Gene ID

53827 (FXYD5)

Available reactivities

Hu (Human), Ms (Mouse), Ye (Yeast)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of Dysadherin / FXYD5 antibody

FXYD5 is a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, has not been characterized as a protein. This reference sequence was derived from AF161462.1 and ESTs; validated by multiple replicate ESTs and human genomic sequence. This gene encodes a member of a family of small membrane proteins that share a 35-amino acid signature sequence domain, beginning with the sequence PFXYD and containing 7 invariant and 6 highly conserved amino acids. The approved human gene nomenclature for the family is FXYD-domain containing ion transport regulator. Mouse FXYD5 has been termed RIC (Related to Ion Channel). FXYD2, also known as the gamma subunit of the Na,K-ATPase, regulates the properties of that enzyme. FXYD1 (phospholemman), FXYD2 (gamma), FXYD3 (MAT-8), FXYD4 (CHIF), and FXYD5 (RIC) have been shown to induce channel activity in experimental expression systems. Transmembrane topology has been established for two family members (FXYD1 and FXYD2), with the N-terminus extracellular and the C-terminus on the cytoplasmic side of the membrane. This gene product, FXYD5, has not been characterized as a protein. Two transcript variants have been found for this gene, and they are both predicted to encode the same protein. [RefSeq curation by Kathleen J. Sweadner, Ph.D., sweadner@helix.mgh.harvard.edu.].

General readings

1. Ino, Y., Gotoh, M., Sakamoto, M., Tsukagoshi, K., Hirohashi, S. Dysadherin, a cancer-associated
cell membrane glycoprotein, down-regulates E-cadherin and promotes metastasis. Proc. Nat. Acad.
Sci. 99: 365-370, 2002.
2. Omasa, T., Chen, Y.-G., Mantalaris, A., Wu, J. H. D. A cDNA from human bone marrow encoding a
protein exhibiting homology to the ATP1-gamma-1/PLM/MAT8 family of transmembrane
proteins. Biochim. Biophys. Acta 1517: 307-310, 2001.
3. Sweadner, K. J., Rael, E. The FXYD gene family of small ion transport regulators or channels:
cDNA sequence, protein signature sequence, and expression. Genomics 68: 41-56, 2000

20 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Dysadherin / FXYD5 (C-term) antibody

TA302772 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Dysadherin / FXYD5 antibody

WB Suggested Anti-FXYD5 Antibody Titration: 0.625ug/ml; ELISA Titer: 1:312500; Positive Control: Human Placenta Rabbit IgG Purified Hu, Ms, Ye WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Dysadherin / FXYD5 antibody

WB Suggested Anti-FXYD5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human heart Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Dysadherin / FXYD5 antibody

WB Suggested Anti-FXYD5 Antibody Titration: 1.25 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Dysadherin / FXYD5 (Center) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue reacted with Dysadherin / FXYD5 Antibody (Center) Cat.-No AP51745PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Dysadherin / FXYD5 antibody

FXYD5 polyclonal antibody ( Cat # PAB6132 ) staining (0.5 µg/ml) of human Spleen lysate ( RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Purified Hu E
0.1 mg / €400.00
  Abnova Taiwan Corp.

Dysadherin / FXYD5 (Center) antibody

Human Heart; WB Suggested Anti-FXYD5 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human heart; FXYD5 antibody - middle region (ARP35225_P050) in Human Heart cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Dysadherin / FXYD5 antibody

Human Jurkat; WB Suggested Anti-FXYD5 Antibody Titration: 1.25ug/ml. Positive Control: Jurkat cell lysate; FXYD5 antibody - middle region (ARP35226_T100) in Human Jurkat cells using Western Blot Rabbit Purified Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

Dysadherin / FXYD5 antibody

Human 293T; . Lanes: Lane 1: 10ug hFXYD5 transfected 293T lysate. Lane 2: 10ug 293T lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:4000. Gene Name: FXYD5. Submitted by: Anonymous.; FXYD5 antibody - N-terminal region (ARP37690_T100) in Human 293T cells using Western Blot Rabbit IgG Purified Hu WB
0.1 mg / €310.00
  AVIVA Systems Biology

Dysadherin / FXYD5 antibody

Dysadherin / FXYD5 Rabbit IgG Aff - Purified Hu P, WB
0.1 mg / €290.00
  Boster Immunoleader

Dysadherin / FXYD5 antibody

Western Blot: Anti-Dysadherin Antibody [NB100-1003]- staining (0.5ug/ml) of Human Spleen lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Dysadherin / FXYD5 antibody

Western Blot: Dysadherin Antibody [NBP1-59137] - This Anti-FXYD5 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1.25ug/ml. Rabbit Purified Hu WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Dysadherin / FXYD5 antibody

Western Blot: Dysadherin Antibody [NBP1-59138] - This Anti-FXYD5 antibody was used in Western Blot of Fetal Heart tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Dysadherin / FXYD5 antibody

Immunohistochemistry-Paraffin: Dysadherin Antibody [NBP1-82486] - Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Dysadherin / FXYD5 (22-145, His-tag)

Dysadherin / FXYD5 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Dysadherin / FXYD5 (22-145, His-tag)

Dysadherin / FXYD5 Human Purified > 95 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

FXYD5 overexpression lysate

FXYD5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

FXYD5 overexpression lysate

FXYD5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

FXYD5 overexpression lysate

FXYD5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

FXYD5 overexpression lysate

FXYD5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

per page
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