Dystrophin / DMD antibody

Principal name

Dystrophin / DMD antibody

SwissProt ID

O97592 (Canfa), P11530 (Rat), P11531 (Mouse), P11532 (Human), P11533 (Chick), Q5GN48 (Pig)

Gene ID

1756 (DMD), 13405 (Dmd)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat), Xen (Xenopus), Por (Porcine), Fi (Fish), Chk (Chicken)

Available hosts

Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Frozen Sections (C), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoelectron Microscopy (EM), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of Dystrophin / DMD antibody

Dystrophin is a muscle membrane protein (427 kDa) which is absent, reduced or altered as a result of mutation in Duchenne and Becker muscular dystrophies (DMD/BMD) or its homologue in the mouse.8 Severe DMD is associated with a marked dystrophin deficiency whereas patients with the milder form of BMD show less pronounced abnormalities of protein expression. Because abnormalities in the protein expression occur specifically in patients with these types of muscular dystrophy, dystrophin analysis may be used to distinguish these conditions from other neuromuscular diseases. Predictions from the sequence suggest a structural protein on the inner face of the membrane, consisting of a 25-repeat, rod-like triple-helical domain separating an N-terminal actin-binding domain from two C-terminal domains, one of which is rich in cysteine.9 The large size of dystrophin and its low abundance (<0.01% of the total muscle protein) are a hindrance to the isolation of intact, native protein for structure/function studies. Monoclonal antibodies against defined regions10 of dystrophin provide a means for studying its structure and function, interactions with other proteins and the nature of the partial gene products produced in some patients carrying deletions in the dystrophin gene. The antibodies are useful in the prenatal or post-abortion diagnosis of muscular dystrophy carriers by immunohistological analyses.11

General readings

This product has been used in:Morris GE et al. A quantitative ELISA for dystrophin. J Immunol Methods 161:23-8 (1993). PubMed PMID: 8486926
Nguyen TM et al. Monoclonal antibodies for dystrophin analysis. Epitope mapping and improved binding to SDS-treated muscle sections. Biochem J 288 (Pt 2):663-8 (1992). PubMed PMID: 1281410
Hugnot JP et al. Distal transcript of the dystrophin gene initiated from an alternative first exon and encoding a 75-kDa protein widely distributed in nonmuscle tissues. Proc Natl Acad Sci U S A 89:7506-10 (1992). PubMed PMID: 1380160
Lederfein D et al. A 71-kilodalton protein is a major product of the Duchenne muscular dystrophy gene in brain and other nonmuscle tissues. Proc Natl Acad Sci U S A 89:5346-50 (1992). PubMed PMID: 1319059
Nguyen thi Man AJ et al. Monoclonal antibodies against defined regions of the muscular dystrophy protein, dystrophin. FEBS Lett 262:237-40 (1990). PubMed PMID: 1692289
General / background references:Gussoni E et al. Normal dystrophin transcripts detected in Duchenne muscular dystrophy patients after myoblast transplantation. Nature 356:435-8 (1992). PubMed PMID: 1557125
Ellis JM et al. Specificity of dystrophin analysis improved with monoclonal antibodies. Lancet 336:881-2 (1990). PubMed PMID: 1699095
1. Ellis, J.M., et al., Lancet, 336, 881 (1990). 2. Nguyen, thi Man, et al., Biochem. J., 288, 663 (1992). 3. Nguyen, thi Man, et al., FEBS Lett., 262, 237 (1990). 4. Hugnot, J.P., et al., Proc. Natl. Acad. Sci. USA, 89, 7506 (1992). 5. Lederfein, D., et al., Proc. Natl. Acad. Sci. USA, 89, 5346 (1992). 6. Gussoni, E., et al., Nature, 356, 435 (1992). 7. Morris, G.E., et al., J. Immunol. Meth., 161, 23 (1993).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Dystrophin / DMD antibody

AP15529PU Dystrophin antibody staining of Human Skeletal Muscle. Rabbit Purified Hu, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Dystrophin / DMD antibody

AP15529PU Dystrophin antibody staining of Human Skeletal Muscle. Rabbit Purified Hu, Rt P, WB
1 ml / €440.00
  Acris Antibodies GmbH

Dystrophin / DMD antibody

AP15529PU Dystrophin antibody staining of Human Skeletal Muscle. Rabbit Purified Hu, Rt P, WB
0.1 ml / €180.00
  Acris Antibodies GmbH

Dystrophin / DMD antibody

Western blot analysis of Dystrophin expression in 3T3 cell lysate (Lane 1) and rat kidney tissue lysate (Lane 2). Rabbit Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Dystrophin / DMD antibody

Dystrophin / DMD Mouse IgG2b DYS-48 Purified Chk, Hu, Ms, Rt P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Dystrophin / DMD antibody

Western Blot analysis of DMD expression in transfected 293T cell line (H00001756-T01) by DMD MaxPab polyclonal antibody.

Lane 1: DMD transfected lysate(72.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Dystrophin / DMD antibody

Dystrophin / DMD Mouse IgG1 3A11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Dystrophin / DMD antibody

Dystrophin / DMD Mouse IgG2b DYS-48 Chk, Hu, Ms, Rt C, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Dystrophin / DMD antibody

Dystrophin / DMD Mouse IgG1 1808 Chk, Fi, Hu, Ms, Rt EM, ICC/IF, P
0.1 mg / €730.00
  Abnova Taiwan Corp.

Dystrophin / DMD antibody

Western blot analysis of DMD expression in NIH/3T3 cell lysate ( Lane 1 ) and rat kidney tissue lysate ( Lane 2 ) . Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Dystrophin / DMD antibody

Dystrophin / DMD Rabbit Hu, Ms P, WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Dystrophin / DMD antibody

Dystrophin / DMD Mouse IgG2b DYS-48 Ascites Chk, Hu, Ms, Rt C, WB
0.1 mg / €290.00
  Boster Immunoleader

Dystrophin / DMD antibody

Immunohistochemistry: Dystrophin Antibody (1808)[NB120-3149]-Formalin fixed paraffin embedded human skeletal muscle stained with Dystrophin antibody Mouse IgG1 1808 Chk, Hu, Ms, Rt, Xen ICC/IF, P
0.5 ml / €400.00
  Novus Biologicals Inc.

Dystrophin / DMD antibody

Dystrophin / DMD Mouse IgG1 MANDRA1 Ascites Fi, Hu, Ms, Rt E, ICC/IF
0.1 ml / €350.00
  Novus Biologicals Inc.

Dystrophin / DMD antibody

Dystrophin / DMD Mouse IgG1 SPM499 Hu, Rt P
0.5 ml / €420.00
  Novus Biologicals Inc.

Dystrophin / DMD antibody

Dystrophin / DMD Mouse IgG1 SPM499 Hu, Rt C, P
1 ml / €460.00
  Novus Biologicals Inc.

Dystrophin / DMD antibody

Dystrophin / DMD Mouse IgG1 SPM499 Hu, Rt C, P
7 ml / €340.00
  Novus Biologicals Inc.

Dystrophin / DMD antibody

Dystrophin / DMD Mouse IgG2b DYS-48 Chk, Hu, Ms, Rt P, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Dystrophin / DMD antibody

Western Blot: Dystrophin Antibody [NBP1-45532] - Western blot analysis of Dystrophin expression in 3T3 cell
lysate (Lane 1) and rat kidney tissue lysate (Lane 2). Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Dystrophin / DMD antibody

Immunohistochemistry-Paraffin: Dystrophin Antibody [NBP1-89953] Immunohistochemical staining of human heart muscle shows distinct membranous positivity. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

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