E2F1 antibody

Principal name

E2F1 antibody

Alternative names for E2F1 antibody

E2F-1, RBBP3, PBR3, Transcription factor E2F1, Retinoblastoma-binding protein 3, PRB-binding protein E2F-1, Retinoblastoma-associated protein 1, E2F transcription factor 1

SwissProt ID

O09139 (Rat), Q01094 (Human), Q61501 (Mouse)

Gene ID

1869 (E2F1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Mky (Monkey), Chimp (Chimpanzee), Chk (Chicken), Rt (Rat), African clawed frog

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Enzyme Immunoassay (E), Dot blot (Dot), Flow Cytometry (F)

Background of E2F1 antibody

E2F transcription factors are important key regulators of cell growth control. There are 5 types of E2F transcription factors (E2F1-E2F5) that play an important role in a number of pathological conditions (1). A double mutant of E2F1/E2F2 mouse has shown to develop non-autoimmune insulin-deficient diabetes and exocrine pancreatic dysfunction with exocrine dysplasia (1). The E2F family of transcription factors consists of two subgroups termed E2F and DP transcription factors that binds DNA cooperatively with dp proteins through the E2 recognition site, TTTCC/GCGC, found in the promoter region of a number of genes. E2F1 is a member of the E2F transcription factors (cell cycle Genes) that are key components of the cyclin D/retinoblastoma/E2F pathway (1). E2F1 binds DPARBR1 proteins. The eukaryotic E2fs are conserved constriction factors which serve an antagonistic role in several pathways related to cellular division, DNA repair, and differentiation (2). Therefore, E2F1 is expressed throughout the cell cycle. E2F1 is one of the key targets for axon to determine whether cells proliferate or whether they exit the cell cycle, enlarge and endoreduplicate their DNA (2).
E2F proteins are also involved in the regulation of human intestinal epithelial cell proliferation and development (3). The expression of E2F1 is decreased and E2F4 protein is sequestered in the cytoplasm during G(0) arrest associated with serum deprivation, confluence, and terminal differentiation of intestinal cells (3). E2F1 deficiency also impairs murine spermatogenesis and augments testicular degeneration. Abundance of E2F1 is increased by auxin trough stabilization of the protein (4). CDK levels are increased by E2F1 even in hormone free conditions. E2F1 activity was shown to induce S phase and endoredupolication. Over expression of E2F1 in transgenic plants lead to modifications of the morphology of the roots, cotyledons, and leaves that can be ascribed to stimulation of cell division. These plants had increased levels of E2F1 as well as ATE2Fb protein which suggests that E2F1expression might be upregulated by Ate2Fa transcription factor. E2F1 is approximately a 56kDa protein, 437 amino acids.

General readings

Cartwright P et al. E2F-6: a novel member of the E2F family is an inhibitor of E2F-dependent transcription. Oncogene 17:611-23 (1998). PubMed PMID: 9704927
Rogers KT et al. DP-2, a heterodimeric partner of E2F: identification and characterization of DP-2 proteins expressed in vivo. Proc Natl Acad Sci U S A 93:7594-9 (1996). PubMed PMID: 8755520
Moberg K et al. E2F-4 switches from p130 to p107 and pRB in response to cell cycle reentry. Mol Cell Biol 16:1436-49 (1996). PubMed PMID: 8657117
Hateboer G et al. Degradation of E2F by the ubiquitin-proteasome pathway: regulation by retinoblastoma family proteins and adenovirus transforming proteins. Genes Dev 10:2960-70 (1996). PubMed PMID: 8956997

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

E2F1 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MYBL2 and E2F1. Mahlavu cells were stained with anti-MYBL2 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 2E10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

E2F1 antibody

Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. TA308764 diluted at 1:500. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

E2F1 pSer364 antibody

WB using E2F-1 pS364 ab shows detection of a ~47 kDa band corresponding to phosphorylated E2F-1 in induced cell lysates. Panel A shows reactivity using a control ab reactive to all forms of E2F (arrowheads). Panel B shows specific reactivity against phosphorylated E2F-1 (arrowheads) using anti-E2F-1 pS364. Lysates are as follows: CRE/E2F-1 are CRE cells derived from mouse NIH3T3 line transfected with human E2F-1, NIH-3T3 used as a negative control, and MDA-MB-231 cells are a human breast cancer line. Lysate was prepared from untreated cells or cells treated with 2 uM Doxorubicin used as DNA damaging agent. MDA-MB-231 cells were also treated with genistein, a mild DNA damaging agent. The figure shows the same membrane first probed with the anti-E2F-1 pS364 at 1:250, then stripped and re-probed with the pan E2F ab used as a positive control. The positive control ab shows an E2F-1 band in all human cell lines, but not mouse cells. Treatment with doxorubicin increases the expression of E2F-1 as shown in Panel A. After film development, images were overlapped to confirm that specific anti-E2F-1 pS364 staining shown treated human cells in Panel B specifically aligns with E2F-1 staining shown in Panel A. Rabbit IgG Aff - Purified Chimp, Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F1 (314-327) antibody

E2F1 antibody (1µg/ml) staining of HeLa nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F1 antibody

Predicted band size: 47 kDa . Positive control: 3T3 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lysate: 30 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

E2F1 antibody

Western blot analysis of E2F1 expression in Etoposide treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

E2F1 antibody

WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Transfected 293TE2F1 is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

E2F1 antibody

WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 ug/ml; Positive Control: Daudi cell lysate E2F1 is strongly supported by BioGPS gene expression data to be expressed in Human Daudi cells Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

E2F1 antibody

Western blot detection of E2F-1 in C6,Raw264.7 and Hela cell lysates using E2F-1 mouse mAb (1:500 diluted).Predicted band size:70KDa.Observed band size:70KDa. Mouse IgG2b 4G8-2C2-C12 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

E2F1 antibody

Western blot analysis of extracts from 3T3 cells using E2F-1 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F1 antibody

Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only. Rabbit IgG Purified Hu E, ICC/IF, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F1 pSer364 antibody

Western blot analysis of phosphorylated E2F1 (Ser364) expression in MCF7. Samples: Crude cell extracts of MCF7 untreated (lane 1) and treated with etoposide at 10 µ M for 16 h (lane 2, 3). Immunoprecipitated with pantropic anti-E2F1 antibody (lane 1, 2) and then analyzed by Western blotting with AM26540AF-S. (Lane 3 is a positive control for Western blotting.) Mouse IgG2b #2 Azide Free Hu WB
50 µg / €410.00
  Acris Antibodies GmbH

E2F1 antibody

Immunohistochemistry analyzes of E2F1 Antibody (Cat.-No AP06386PU-N) in paraffin-embedded Human brain tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

E2F1 pSer364 (360-369) antibody

AP07709PU-N E2F1 antibody staining of Formalin-Fixed Paraffin-Embedded Human Breast Carcinoma at 10 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit Aff - Purified Hu E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

E2F1 control peptide

E2F1
0.25 mg / €250.00
  Acris Antibodies GmbH

E2F1 (58-83) antibody

E2F1 Rabbit Aff - Purified Hu E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

E2F1 control peptide

E2F1
0.25 mg / €250.00
  Acris Antibodies GmbH

E2F1 (138-144) antibody

Western blot with AP09982PU-N  at 1:500. Rabbit Aff - Purified Hu E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

E2F1 control peptide

E2F1
0.25 mg / €250.00
  Acris Antibodies GmbH

E2F1 (C-term) antibody

E2F1 Rabbit Aff - Purified Hu E, IP, WB
0.25 mg / €420.00
  Acris Antibodies GmbH

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