E2F2 antibody

Principal name

E2F2 antibody

Alternative names for E2F2 antibody

E2F-2, Transcription factor E2F2, E2F transcription factor 2

SwissProt ID

P56931 (Mouse), Q14209 (Human)

Gene ID

1870 (E2F2)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), African clawed frog, Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of E2F2 antibody

E2F2 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small D tumor viruses. The E2F proteins contain several evolutiolly conserved domains found in most members of the family. These domains include a D binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additiol cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small D tumor viruses. The E2F proteins contain several evolutiolly conserved domains found in most members of the family. These domains include a D binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F3, have an additiol cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additiol publications.

General readings

General / background references:Nevins JR Toward an understanding of the functional complexity of the E2F and retinoblastoma families. Cell Growth Differ 9:585-93 (1998). PubMed PMID: 9716176
Weinberg RA The retinoblastoma protein and cell cycle control. Cell 81:323-30 (1995). PubMed PMID: 7736585
Weintraub SJ et al. Mechanism of active transcriptional repression by the retinoblastoma protein. Nature 375:812-5 (1995). PubMed PMID: 7596417
Zhang Y & Chellappan SP Cloning and characterization of human DP2, a novel dimerization partner of E2F. Oncogene 10:2085-93 (1995). PubMed PMID: 7784053

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

E2F2 antibody

Western blot analysis of extracts from HepG2 cells, K562 cells and COLO205 cells, using E2F2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

E2F2 antibody

Predicted band size: 48 kDa . Positive control: A549 cell lysate. Recommended dilution: 1/500-2000. (Gel: 15%+12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

E2F2 antibody

WB Suggested Anti-E2F2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

E2F2 antibody

Western blot analysis of extracts from Hela and K562 cells using E2F-2 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F2 (202-252) antibody

Western blot analysis of E2F2 Antibody Cat.-No AP06731PU-N in extracts from HepG2 cells. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

E2F2 control peptide

E2F2
0.25 mg / €250.00
  Acris Antibodies GmbH

E2F2 (73-99) antibody

Western blot of E2F2 with AP09984PU-N (1:500). Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

E2F2 (Center) antibody

Western blot analysis of E2F2 Antibody (Center) Cat.-No AP51350PU-N in mouse kidney tissue lysates (35ug/lane). This demonstrates the E2F2 antibody detected the E2F2 protein (arrow). Rabbit Ig Aff - Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

E2F2 antibody

E2F2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

E2F2 antibody

Western Blot analysis of E2F2 expression in transfected 293T cell line (H00001870-T01) by E2F2 MaxPab polyclonal antibody.

Lane 1: E2F2 transfected lysate(9.24 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

E2F2 antibody

Western Blot analysis of E2F2 expression in transfected 293T cell line (H00001870-T01) by E2F2 MaxPab polyclonal antibody.

Lane 1: E2F2 transfected lysate(9.24 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

E2F2 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between E2F2 and E2F1 HeLa cells were stained with anti-E2F2 rabbit purified polyclonal 1:1200 and anti-E2F1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

E2F2 antibody

Western blot analysis of extracts from HepG2/K562/COLO205 cells, using E2F2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

E2F2 antibody

Western blot analysis of extracts from HepG2/K562/COLO205 cells, using E2F2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

E2F2 antibody

Human Jurkat; WB Suggested Anti-E2F2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate. E2F2 is supported by BioGPS gene expression data to be expressed in Jurkat.; E2F2 antibody - middle region (ARP31611_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

E2F2 antibody

Human Brain; Immunohistochemistry with Brain, cerebellum tissue at an antibody concentration of 5ug/ml using anti-E2F2 antibody (ARP38418_P050); E2F2 antibody - middle region (ARP38418_P050) in Human Brain cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

E2F2 antibody

E2F2 Rabbit IgG Aff - Purified Hu, Ms, Rt WB
0.1 mg / €290.00
  Boster Immunoleader

E2F2 antibody

Immunohistochemical analysis of E2F2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

E2F2 antibody

Immunohistochemical analysis of E2F2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

E2F2 antibody

Immunohistochemical analysis of E2F2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

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