E2F3 antibody

Principal name

E2F3 antibody

Alternative names for E2F3 antibody

E2F-3, KIAA0075, MGC104598, DKFZp686C18211, Transcription factor E2F3, E2F transcription factor 3

SwissProt ID

O00716 (Human), O35261 (Mouse)

Gene ID

1871 (E2F3), 13557 (E2f3)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), African clawed frog, Xen (Xenopus), Eq (Equine), GP (Guinea Pig), Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Electrophoretic Mobility Shift Assay (EMSA), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Frozen Sections (C), ChIP assay (ChIP)

Background of E2F3 antibody

E2F transcription factors are important key regulators of cell growth control. Multiple types of E1F factors are cloned and characterized that fall in 2 functional categories, based on current evidence, the E2Fs can be functionally divided in to Transcriptional activators (E2F1, E2F2, E2F3a) ir repressors (E2F3b, E2F4, E2F5, F2F6 and E2F7). Recently a novel E2F8 factors is also characterized that share common structural motifs with E2F7 such as presence of 2 distinct DNA biding dominos, and the DP-dimmerization domains. E2F transcription factors are known to play important role in a number of pathological conditions. The E2F family of transcription factors consists of two subgroups termed E2F and DP transcription factors that binds DNA cooperatively with dp proteins through the E2 recognition site, TTTCC/GCGC, found in the promoter region of a number of genes. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Deregulation of the Rb-E2F1 pathway also results in the activation of p53 and the promotion of apoptosis, and this led to the suppression of tumor development (2). A double mutant of E2F1/E2F2 mouse has shown to develop non-autoimmune insulin-deficient diabetes and exocrine pancreatic dysfunction with exocrine dysplasia (1).
E2F proteins are also involved in the regulation of human intestinal epithelial cell proliferation and development. The expression of E2F1 is decreased and E2F4 protein is sequestered in the cytoplasm during G(0) arrest associated with serum deprivation, confluence, and terminal differentiation of intestinal cells (3). E2F1 deficiency also impairs murine spermatogenesis and augments testicular degeneration. E2F-1, E2F-2 and E2F-5 are first detected in the 9.5 days post-coitus (dpc) forebrain. Expression of these E2F forms extends caudally thereafter and includes the developing brain and the upper half of the 10.5 dpc spinal cord (4).

General readings

General / background references:Nevins JR Toward an understanding of the functional complexity of the E2F and retinoblastoma families. Cell Growth Differ 9:585-93 (1998). PubMed PMID: 9716176
Weinberg RA The retinoblastoma protein and cell cycle control. Cell 81:323-30 (1995). PubMed PMID: 7736585
Weintraub SJ et al. Mechanism of active transcriptional repression by the retinoblastoma protein. Nature 375:812-5 (1995). PubMed PMID: 7596417
Zhang Y & Chellappan SP Cloning and characterization of human DP2, a novel dimerization partner of E2F. Oncogene 10:2085-93 (1995). PubMed PMID: 7784053

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

E2F3 (C-term) antibody

E2F3 antibody (1µg/ml) staining of Mouse fetal Lung lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F3 antibody

WB Suggested Anti-E2F3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: Human Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

E2F3 antibody

Western blot analysis of E2F3 in human lymph node tissue lysate with E2F3 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F3 control peptide

E2F3
0.25 mg / €250.00
  Acris Antibodies GmbH

E2F3 (70-82) antibody

E2F3 Rabbit Aff - Purified Ms E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

E2F3 antibody

E2F3 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

E2F3 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between MSH2 and E2F3 HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-E2F3 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG1 5F7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

E2F3 antibody

E2F3 Mouse IgG2b 3C11 Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

E2F3 antibody

Human Thymus; WB Suggested Anti-E2F3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Thymus; E2F3 antibody - C-terminal region (ARP38089_P050) in Human Thymus cells using Western Blot Rabbit IgG Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

E2F3 antibody

E2F3 Rabbit IgG Aff - Purified Hu, Ms WB
0.1 mg / €290.00
  Boster Immunoleader

E2F3 antibody

E2F3 Rabbit IgG Aff - Purified Ms WB
50 µg / €680.00
  Funakoshi Co., Ltd.

E2F3 antibody

E2F3 Mouse IgG2a PG30 Purified Hu IP, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

E2F3 antibody

Western Blot: E2F3 Antibody [NBP1-80295] Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt, Xen WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

E2F3 (full length, N-term HIS tag)

E2F3 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

E2F3

E2F3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

E2F3

E2F3 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

E2F3

E2F3 Human
10 µg / €380.00
  Abnova Taiwan Corp.

E2F3

E2F3 Human
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

E2F3 overexpression lysate

E2F3 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

19 Item(s)

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