E2F4 antibody

Principal name

E2F4 antibody

Alternative names for E2F4 antibody

E2F-4, Transcription factor E2F4, p107/p130-binding, E2F transcription factor 4

SwissProt ID

Q16254 (Human), Q8R0K9 (Mouse)

Gene ID

1874 (E2F4), 104394 (E2f4)

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Ze (Zebrafish), African clawed frog, Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection)), PLA/Proximity ligation assay (PLA)

Background of E2F4 antibody

E2F transcription factors are important key regulators of cell growth control. Multiple types of E1F factors are cloned and characterized that fall in 2 functional categories, based on current evidence, the E2Fs can be functionally divided in to Transcriptional activators (E2F1, E2F2, E2F3a) ir repressors (E2F3b, E2F4, E2F5, F2F6 and E2F7). Recently a novel E2F8 factors is also characterized that share common structural motifs with E2F7 such as presence of 2 distinct DNA biding dominos, and the DP-dimmerization domains. E2F transcription factors are known to play important role in a number of pathological conditions. The E2F family of transcription factors consists of two subgroups termed E2F and DP transcription factors that binds DNA cooperatively with dp proteins through the E2 recognition site, TTTCC/GCGC, found in the promoter region of a number of genes. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Deregulation of the Rb-E2F1 pathway also results in the activation of p53 and the promotion of apoptosis, and this led to the suppression of tumor development (2). A double mutant of E2F1/E2F2 mouse has shown to develop non-autoimmune insulin-deficient diabetes and exocrine pancreatic dysfunction with exocrine dysplasia (1).
E2F proteins are also involved in the regulation of human intestinal epithelial cell proliferation and development. The expression of E2F1 is decreased and E2F4 protein is sequestered in the cytoplasm during G(0) arrest associated with serum deprivation, confluence, and terminal differentiation of intestinal cells (3). E2F1 deficiency also impairs murine spermatogenesis and augments testicular degeneration. E2F-1, E2F-2 and E2F-5 are first detected in the 9.5 days post-coitus (dpc) forebrain. Expression of these E2F forms extends caudally thereafter and includes the developing brain and the upper half of the 10.5 dpc spinal cord (4).

General readings

1. Müller, H., et al., Mol. Cell Biol. 17, 5508-5520 (1997)
Clone TFE42 is used in this reference.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

E2F4 antibody

Western blot analysis of extracts from COS7 cells, using E2F4 antibody (#TA313778).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

E2F4 antibody

E2F4 antibody (2µg/ml) staining of Human Brain (Cerebellum) lysate (35µg protein in RIPA buffer) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F4 antibody

E2F4 antibody (0.3µg/ml) staining of A43 (A) and HeLa (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F4 antibody

Western blot analysis on COS7 cell lysate using E2F4 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F4 antibody

Figure 1. Western blot analysis of E2F4 expression in HL60 (Lane 1), Raji (Lane 2), NIH/3T3 (Lane 3), C2C12 (Lane 4) and Rat-1 (Lane 5) using E2F4 antibody AM00536AF-N. Mouse IgG1 TFE42 Azide Free Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

E2F4 Control Peptide

E2F4
0.25 mg / €250.00
  Acris Antibodies GmbH

E2F4 antibody

E2F4 Rabbit Aff - Purified Ms E, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

E2F4 antibody

DM382 E2F4 antibody staining of Formalin-Fixed, Paraffin Embedded Human tonsil using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells. Mouse IgG1 4E2F04 Purified Hu, Rt ICC/IF, P, WB
1 ml / €730.00
  Acris Antibodies GmbH

E2F4 antibody

DM382 E2F4 antibody staining of Formalin-Fixed, Paraffin Embedded Human tonsil using peroxidase-conjugate and AEC chromogen. Note nuclear staining of cells. Mouse IgG1 4E2F04 Purified Hu, Rt ICC/IF, P, WB
0.5 ml / €470.00
  Acris Antibodies GmbH

E2F4 antibody

Western Blot analysis of E2F4 expression in transfected 293T cell line (H00001874-T01) by E2F4 MaxPab polyclonal antibody.

Lane 1: E2F4 transfected lysate(45.43 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

E2F4 antibody

E2F4 MaxPab rabbit polyclonal antibody. Western Blot analysis of E2F4 expression in mouse spleen. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

E2F4 antibody

E2F4 monoclonal antibody (M01), clone 5B7 Western Blot analysis of E2F4 expression in A-549 ( Cat # L025V1 ). Mouse IgG1 5B7 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

E2F4 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using E2F4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

E2F4 antibody

Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using E2F4 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

E2F4 antibody

Human Intestine; E2F4 antibody - C-terminal region (ARP31175_P050) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze P, WB
50 µg / €350.00
  AVIVA Systems Biology

E2F4 antibody

Immunohistochemical analysis of E2F4 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

E2F4 antibody

Immunohistochemical analysis of E2F4 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

E2F4 antibody

Immunohistochemical analysis of E2F4 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Chk, Hu, Ms, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

E2F4 antibody

Immunohistochemistry: E2F4/E2F5 Antibody [NB100-91738] -  E2F4/E2F5 antibody (L50/83) [NB100-91738] - in paraffin-embedded human lung carcinoma tissue. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

E2F4 antibody

Human tonsil stained with anti-E2F-4 Mouse IgG1 SPM179 Serum Hu, Rt ICC/IF, P, WB
0.5 ml / €400.00
  Novus Biologicals Inc.

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