E2F5 antibody

Principal name

E2F5 antibody

Alternative names for E2F5 antibody

E2F-5, Transcription factor E2F5, E2F transcription factor 5, p130-binding

SwissProt ID

Q15329 (Human), Q61502 (Mouse), Q62814 (Rat)

Gene ID

1875 (E2F5)

Available reactivities

Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C), Paraffin Sections (P)

Background of E2F5 antibody

E2F transcription factors are important key regulators of cell growth control. Multiple types of E1F factors are cloned and characterized that fall in 2 functional categories, based on current evidence, the E2Fs can be functionally divided in to Transcriptional activators (E2F1, E2F2, E2F3a) ir repressors (E2F3b, E2F4, E2F5, F2F6 and E2F7). Recently a novel E2F8 fctors is also characterized that share common structural motifs with E2F7 such as presence of 2 distinct DNA biding dominos, and the DP-dimmerization domains. E2F transcription factors are known to play important role in a number of pathological conditions. The E2F family of transcription factors consists of two subgroups termed E2F and DP transcription factors that binds DNA cooperatively with dp proteins through the E2 recognition site, TTTCC/GCGC, found in the promoter region of a number of genes. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Deregulation of the Rb-E2F1 pathway also results in the activation of p53 and the promotion of apoptosis, and this led to the suppression of tumor development (2). A double mutant of E2F1/E2F2 mouse has shown to develop non-autoimmune insulin-deficient diabetes and exocrine pancreatic dysfunction with exocrine dysplasia (1).
E2F proteins are also involved in the regulation of human intestinal epithelial cell proliferation and development. The expression of E2F1 is decreased and E2F4 protein is sequestered in the cytoplasm during G(0) arrest associated with serum deprivation, confluence, and terminal differentiation of intestinal cells (3). E2F1 deficiency also impairs murine spermatogenesis and augments testicular degeneration. E2F-1, E2F-2 and E2F-5 are first detected in the 9.5 days post-coitus (dpc) forebrain. Expression of these E2F forms extends caudally thereafter and includes the developing brain and the upper half of the 10.5 dpc spinal cord (4).

General readings

Ohtani,N., et al., (2003) J. Cell Biol. 162 (2), 173-183

11 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

E2F5 antibody

WB Suggested Anti-E2F5 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

E2F5 control peptide

E2F5
0.25 mg / €250.00
  Acris Antibodies GmbH

E2F5 (9-27) antibody

E2F5 Rabbit Aff - Purified Hu E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

E2F5 antibody

Western blot analysis of E2F-5 antibody (Cat.-No.: AP20621PU-N) in extracts from HeLa cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

E2F5 antibody

E2F5 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

E2F5 antibody

Human Jurkat; WB Suggested Anti-E2F5 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; E2F5 antibody - N-terminal region (P100920_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

E2F5 antibody

Western Blot: E2F5 Antibody [NBP1-51239] - Analysis of E2F-5 (L142) pAb in extracts from HeLa cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

E2F5 (transcript variant 1)

E2F5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

E2F5 (N-term HIS tag, transcript variant 1)

E2F5 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

E2F5 overexpression lysate

E2F5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

E2F5 overexpression lysate

E2F5 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

11 Item(s)

per page
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