E2F7 antibody

Principal name

E2F7 antibody

Alternative names for E2F7 antibody

E2F-7, FLJ12981, Transcription factor E2F7, E2F transcription factor 7

SwissProt ID

D4A4D7, E1BE02, F6YVB9, Q5RIX9, Q6S7F2 (Mouse), Q96AV8 (Human)

Gene ID

52679 (E2f7), 144455 (E2F7)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation (IP), Enzyme Immunoassay (E)

Background of E2F7 antibody

Atypical E2F transcription factor that participates in various processes such as angiogenesis, polyploidization of specialized cells and D damage response. Mainly acts as a transcription repressor that binds D independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1. Acts as a regulator of S-phase by recognizing and binding the E2-related site 5'-TTCCCGCC-3' and mediating repression of G1/S-regulated genes. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Also involved in D damage response: up-regulated by p53/TP53 following genotoxic stress and acts as a downstream effector of p53/TP53-dependent repression by mediating repression of indirect p53/TP53 target genes involved in D replication. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene. Acts as a negative regulator of keratinocyte differentiation.

General readings

de Bruin,A., et al., (2003) J. Biol. Chem. 278 (43), 42041-42049

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

E2F7 antibody

TA305912 (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Por E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

E2F7 antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human gastric and colon cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human gastric cancer tissue using E2F7 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

E2F7 antibody

Host: Rabbit; Target Name: E2f7; Sample Tissue: Rat Testis lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

E2F7 (Center) antibody

Western blot analysis of E2F7 Antibody Cat.-No AP51351PU-N in A2058 cell line lysates (35ug/lane). This demonstrates the E2F7 antibody detected the E2F7 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

E2F7 antibody

E2F7 polyclonal antibody ( Cat # PAB12815 ) (0.1 µg/ml) staining of human kidney lysate ( 35 µg protein in RIPA buffer ). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

E2F7 (Isoform 2 specific) antibody

The anti-E2F7-A BCN4774 antibody is able to supershift a DNA-protein complex that contains E2F7-A. Complex formation employing a radiolabeled E2F element from the human DHFR promoter and nuclear extracts from mocktransfected Saos-2 cells (-) or nuclear extracts from Saos-2 cells transfected with pcDNA3-HAE2F7- A (+) were analyzed by EMSA. Extracts were preincubated in the absence (None) or in the presence of an antibody to the HA tag or the anti-E2F7-A BCN4774 antibody. The arrow indicates the position of the HA-E2F7-Acontaining complex, whereas arrowheads indicate the position of supershifted complexes. Rabbit Serum Hu EMSA, IP, WB
0.1 ml / €360.00
  Antibody BCN

E2F7 (Isoform 1 specific) antibody

The anti-E2F7-B BCN4775 antibody is able to supershift a DNA-protein complex that contains E2F7-B. Complex formation employing a radiolabeled E2F element from the human DHFR promoter and nuclear extracts from Saos-2 cells transfected with pcDNA3-HAE2F7- B (+) were analyzed by EMSA. Extracts were preincubated in the absence (None) or in the presence of an antibody to the HA tag,anti-E2F7-B BCN4775 antibody, or the corresponding pre-immune serum (PI). The arrow indicates the position of the HA-E2F7-B-containing complex, whereas arrowheads indicate the position of super-shifted complexes. Rabbit Serum Hu EMSA, IP, WB
0.1 ml / €360.00
  Antibody BCN

E2F7 (Isoform 1+2) antibody

The anti-E2F7 BCN4776 antibody is able to supershift DNA-protein complexes that contain either E2F7-A or E2F7-B. Complex formation employing a radiolabeled E2F element from the human DHFR promoter and nuclear extracts from Saos-2 cells transfected with either pcDNA3-HA-E2F7-A (A) or pcDNA3-HA-E2F7-B (B) were analyzed by EMSA. Extracts were preincubated in the presence of an antibody to the HA tag, the anti-E2F7-B (BCN4775), the anti-E2F7 BCN4776 antibody, or the corresponding pre-immune serum (PI). The arrow indicates the position of the HA-E2F7-A- or HA-E2F7-B-containing complexes, whereas arrowheads indicate the position of super-shifted complexes. Rabbit Serum Hu EMSA, IP, WB
0.1 ml / €360.00
  Antibody BCN

E2F7 antibody

Mouse stomach; E2f7 antibody - N-terminal region (ARP37583_P050) in Mouse stomach cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

E2F7 antibody

Mouse SP2/0; WB Suggested Anti-E2F7 Antibody Titration: 2.5ug/ml. ELISA Titer: 1:312500. Positive Control: SP2/0 cell lysate; E2F7 antibody - N-terminal region (ARP37584_T100) in Mouse SP2/0 cells using Western Blot Rabbit Aff - Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

E2F7 antibody

Western Blot: E2F7 Antibody [NBP1-26399]- (0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Goat IgG Aff - Purified Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

E2F7 antibody

Western Blot: E2F7 Antibody [NBP1-52648]-Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells.   Affinity purified rabbit anti-E2F7 antibody  used for IP at 6 mcg/mg lysate.  E2F7 was also immunoprecipitated by rabbit anti-E2F7 antibody NBP1-52649, which recognizes a downstream epitope. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

E2F7 antibody

Western Blot: E2F7 Antibody [NBP1-52649]-Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells.   Affinity purified rabbit anti-E2F7 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  E2F7 was also immunoprecipitated by rabbit anti-E2F7 antibody NBP1-52648, which recognizes an upstream epitopes. Rabbit Aff - Purified Hu IP, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

E2F7 antibody

E2F7 Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €440.00
  Novus Biologicals Inc.

E2F7 antibody

E2F7 Rabbit Purified Bov, Can, Chk, Hu, Ms, Rt WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

E2F7

E2F7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

E2F7

E2F7 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

E2F7 overexpression lysate

E2F7 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

18 Item(s)

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