Early endosome antigen 1 antibody

Principal name

Early endosome antigen 1 antibody

Alternative names for Early endosome antigen 1 antibody

EEA1, ZFYVE2, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

SwissProt ID

Q15075 (Human), Q8BL66 (Mouse)

Gene ID

8411 (EEA1), 216238 (Eea1)

Available reactivities

Can (Canine), Hst (Hamster), Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), African clawed frog, Mky (Monkey), Por (Porcine), Bov (Bovine), Eq (Equine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat, Chicken

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Dot blot (Dot)

Background of Early endosome antigen 1 antibody

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc finger like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl terminal domains of EEA1. The carboxyl terminal zinc finger like domain is conserved in several other non nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3 kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes. Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.

General readings

Fratti,R.A., et al., (2003) J. Biol. Chem. 278 (47), 46961-46967

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Early endosome antigen 1 (N-term) antibody

Early endosome antigen 1 Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Early endosome antigen 1 (C-term) antibody

Early endosome antigen 1 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
20 µl / €50.00
  SICGEN

Early endosome antigen 1 (C-term) antibody

Early endosome antigen 1 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.6 mg / €320.00
  SICGEN

Early endosome antigen 1 antibody

Predicted band size: 162 kDa . Positive control: Jurkat, HL-60 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: Jurkat, HL-60 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Early endosome antigen 1 (821-835) antibody

TA329677 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Early endosome antigen 1 antibody

WB Suggested Anti-EEA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Early endosome antigen 1 antibody

WB Suggested Anti-EEA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: 721_B cell lysateEEA1 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, Hu, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

Early endosome antigen 1 (C-term) antibody

Early endosome antigen 1 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
20 µl / €50.00
  SICGEN

Early endosome antigen 1 (C-term) antibody

Early endosome antigen 1 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.6 mg / €320.00
  SICGEN

Early endosome antigen 1 antibody

EEA1 polyclonal antibody (A01), Lot # 060717JCS1. Western Blot analysis of EEA1 expression in NIH/3T3. Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Early endosome antigen 1 antibody

EEA1 monoclonal antibody (M03), clone 2G2. Western Blot analysis of EEA1 expression in A-431 ( Cat # L015V1 ). Mouse IgG1 2G2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Early endosome antigen 1 antibody

Human Adult Liver; Rabbit Anti-EEA1 Antibody . Catalog Number: ARP30074_P050. Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver . Observed Staining: Cytoplasm in hepatocytes, moderate signal, moderate tissue distribution. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20X. Exposure Time: 0.5 – 2.0 sec. Protocol located in Reviews and Data.; EEA1 antibody - N-terminal region (ARP30074_P050) in Human Adult Liver cells using Immunohistochemistry Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

Early endosome antigen 1 antibody

Human Adult Liver; Rabbit Anti-EEA1 Antibody . Catalog Number: ARP58125_P050. Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver . Observed Staining: Cytoplasm in hepatocytes, moderate signal, moderate tissue distribution. Primary Antibody Concentration: 1:100. Secondary Antibody: Donkey anti-Rabbit-Cy3. Secondary Antibody Concentration: 1:200. Magnification: 20X. Exposure Time: 0.5 – 2.0 sec. Protocol located in Reviews and Data.; EEA1 antibody - middle region (ARP58125_P050) in Human Adult Liver cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Chk, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Early endosome antigen 1 (N-term) antibody

Human HepG2; WB Suggested Anti-EEA1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: HepG2 cell lysate; EEA1 antibody - N-terminal region (ARP58126_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Early endosome antigen 1 antibody

Western blot analysis of EEA1 expression in A431 (A); HeLa (B); THP1 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Por, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Early endosome antigen 1 antibody

Western blot analysis of EEA1 expression in A431 (A); HeLa (B); THP1 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Por, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Early endosome antigen 1 antibody

Western blot analysis of EEA1 expression in A431 (A); HeLa (B); THP1 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Por, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Early endosome antigen 1 antibody

Western Blot and Immunoprecipitation: EEA1 Antibody [NBP1-05961]- Whole cell lysate from HeLa and 293T cells. EEA1 was also immunoprecipitated by rabbit anti-EEA1 antibodies NBP1-05962 and NBP1-05963. Rabbit IgG Aff - Purified Hu IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Early endosome antigen 1 antibody

Western Blot and Immunoprecipitation: EEA1 Antibody [NBP1-05962]- Whole cell  lysate from HeLa, 293T, and mouse NIH3T3 cells. EEA1 was also immunoprecipitated by rabbit anti-EEA1 antibodies NBP1-05961 and NBP1-05963. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

Early endosome antigen 1 antibody

Immunofluorescence: EEA1 Antibody [NBP1-30914] - Immunofluorescence analysis of HeLa, using NBP1-30914 at 1:200 dilution. Rabbit IgG Hu, Ms Dot, ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

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