Early endosome antigen 1 antibody

Principal name

Early endosome antigen 1 antibody

Alternative names for Early endosome antigen 1 antibody

EEA1, ZFYVE2, Endosome-associated protein p162, Zinc finger FYVE domain-containing protein 2

SwissProt ID

Q15075 (Human), Q8BL66 (Mouse)

Gene ID

8411 (EEA1), 216238 (Eea1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), African clawed frog, Can (Canine), Mky (Monkey), Por (Porcine), Bov (Bovine), Eq (Equine), Rb (Rabbit)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP)

Background of Early endosome antigen 1 antibody

Early endosomal antigen 1 (EEA1) is a 162 kDa membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes

General readings

Fratti,R.A., et al., (2003) J. Biol. Chem. 278 (47), 46961-46967

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Early endosome antigen 1 (N-term) antibody

Early endosome antigen 1 Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

Early endosome antigen 1 (C-term) antibody

Early endosome antigen 1 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
20 µl / €50.00
  SICGEN

Early endosome antigen 1 (C-term) antibody

Early endosome antigen 1 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.6 mg / €320.00
  SICGEN

Early endosome antigen 1 antibody

  Anti-EEA1 Rabbit Polyclonal Antibody  
Predicted band size: 162 kDa . Positive control: Jurkat, HL-60 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: Jurkat, HL-60 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Early endosome antigen 1 antibody

  Rabbit Polyclonal anti-EEA1 antibody  
WB Suggested Anti-EEA1 Antibody Titration: 0.06ug/ml; ELISA Titer: 1:312500; Positive Control: Human Placenta Rabbit IgG Purified Hu, Rt IP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Early endosome antigen 1 (821-835) antibody

  Goat Anti-EEA1 (aa821-835) Polyclonal Antibody  
TA329677 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Early endosome antigen 1 antibody

  Rabbit Polyclonal Anti-EEA1 Antibody - N-terminal region  
WB Suggested Anti-EEA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Early endosome antigen 1 antibody

  Rabbit Polyclonal Anti-EEA1 Antibody - middle region  
WB Suggested Anti-EEA1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: 721_B cell lysateEEA1 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Bov, Can, Eq, Hu, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

Early endosome antigen 1 (C-term) antibody

Early endosome antigen 1 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
20 µl / €50.00
  SICGEN

Early endosome antigen 1 (C-term) antibody

Early endosome antigen 1 Goat IgG Aff - Purified Can, Hu, Mky, Ms, Rt ICC/IF, WB
0.6 mg / €320.00
  SICGEN

CRISPR

Catalog No. Species Donor Vector  

EEA1 - human gene knockout kit via CRISPR

EEA1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EEA1 - human gene knockout kit via CRISPR

EEA1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EEA1 - human gene knockout kit via CRISPR

EEA1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EEA1 - human gene knockout kit via CRISPR

EEA1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Eea1 - mouse gene knockout kit via CRISPR

Eea1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Eea1 - mouse gene knockout kit via CRISPR

Eea1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Early endosome antigen 1

  Recombinant protein of human early endosome antigen 1 (EEA1)  
Early endosome antigen 1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

17 Item(s)

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