EBAG9 / RCAS1 antibody

Principal name

EBAG9 / RCAS1 antibody

Alternative names for EBAG9 / RCAS1 antibody

EB9, PDAF, Receptor-binding cancer antigen expressed on SiSo cells, Cancer-associated surface antigen RCAS1, Estrogen receptor-binding fragment-associated gene 9 protein

SwissProt ID

O00559 (Human), Q5PQP2 (Rat), Q9D0V7 (Mouse)

Gene ID

9166 (EBAG9), 55960 (Ebag9), 299864

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Bt (Bat), Can (Canine), Chk (Chicken), Eq (Equine), Fi (Fish), Mky (Monkey), Op (Opossum), Por (Porcine), Prim (Primate), Rb (Rabbit), Xen (Xenopus), Tr (Turkey), L (Lizard), Bear, GP (Guinea Pig), Ye (Yeast), Ze (Zebrafish)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Flow Cytometry (F)

Background of EBAG9 / RCAS1 antibody

RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) is a novel tumoreassociated antigen expressed in human uterine and ovarian carcinomas. The predicted amino acid sequence of RCAS1 (213 a.a.) possesses an N-terminal transmembrane region and a coiled-coil structure in the C-terminal portion, indicating that RCAS1 is a type II membrane protein able to form oligomers through the coiled-coil structure. RCAS1 revealed different expression pattern from the known tumore associated antigens such as, YH206, GA733, CA125, CEA and sialyl Le molecules in human tumore cell lines. Recent study indicated RCAS1 acts as a ligand for a putative receptor present on various human cells including T, B and NK cells. RCAS1 inhibited the in vitro growth of receptor-expressing cells and induced apoptosis. It was suggested that tumore cells might evade immune surveillance by expression of RCAS1.

General readings

Kumagai J., et al.,(2009) Int J Cancer.124(4):816-26.
Yoshida S., et al.,(2008) J Rheumatol. 35(9):1716-22.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EBAG9 / RCAS1 (C-term) antibody

  Goat Polyclonal Antibody against EBAG9  
TA303119 (0.2µg/ml) staining of A431 lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide.  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EBAG9 / RCAS1 antibody

  Rabbit Polyclonal Anti-EBAG9 Antibody  
WB Suggested Anti-EBAG9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EBAG9 / RCAS1 antibody

  Rabbit Polyclonal anti-RCAS1 Antibody  
Human Breast Carcinoma stained with anti-RCAS1 antibody. Rabbit Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EBAG9 / RCAS1 antibody

IHC on human uterine cervical adenocarcinoma Mouse IgM 22-1-1 Azide Free Hu F, P
0.1 ml / €420.00
  Acris Antibodies GmbH

EBAG9 / RCAS1 (240-251) antibody

Skeletal muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Ajellomyces, Bov, Bt, Can, Chk, Elephant, Eq, Hu, Mky, Ms, Por, Rb E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

EBAG9 - human gene knockout kit via CRISPR

EBAG9 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EBAG9 - human gene knockout kit via CRISPR

EBAG9 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EBAG9 - human gene knockout kit via CRISPR

EBAG9 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EBAG9 - human gene knockout kit via CRISPR

EBAG9 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ebag9 - mouse gene knockout kit via CRISPR

Ebag9 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ebag9 - mouse gene knockout kit via CRISPR

Ebag9 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ebag9 - mouse gene knockout kit via CRISPR

Ebag9 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Ebag9 - mouse gene knockout kit via CRISPR

Ebag9 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EBAG9 / RCAS1 (transcript variant 1)

  Recombinant protein of human estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 1  
EBAG9 / RCAS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EBAG9 / RCAS1 (transcript variant 2)

  Recombinant protein of human estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 2  
EBAG9 / RCAS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EBAG9 / RCAS1 (28-213, His-tag)

EBAG9 / RCAS1 Human Purified > 90 % by SDS PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

EBAG9 / RCAS1 (28-213, His-tag)

EBAG9 / RCAS1 Human Purified > 90 % by SDS PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, EBAG9 (mGFP-tagged) - Human estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, EBAG9 (mGFP-tagged) - Human estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, EBAG9 (Myc-DDK tagged) - Human estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, EBAG9 (Myc-DDK tagged) - Human estrogen receptor binding site associated, antigen, 9 (EBAG9), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ebag9 (GFP-tagged) - Mouse estrogen receptor-binding fragment-associated gene 9 (Ebag9), 200 uL, >10^7 TU/mL

Lenti ORF particles, Ebag9 (GFP-tagged) - Mouse estrogen receptor-binding fragment-associated gene 9 (Ebag9), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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