EBAG9 / RCAS1 antibody

Principal name

EBAG9 / RCAS1 antibody

Alternative names for EBAG9 / RCAS1 antibody

EB9, PDAF, Receptor-binding cancer antigen expressed on SiSo cells, Cancer-associated surface antigen RCAS1, Estrogen receptor-binding fragment-associated gene 9 protein

SwissProt ID

O00559 (Human), Q5PQP2 (Rat), Q9D0V7 (Mouse)

Gene ID

9166 (EBAG9)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F)

Background of EBAG9 / RCAS1 antibody

RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) is a novel tumoreassociated antigen expressed in human uterine and ovarian carcinomas. The predicted amino acid sequence of RCAS1 (213 a.a.) possesses an N-terminal transmembrane region and a coiled-coil structure in the C-terminal portion, indicating that RCAS1 is a type II membrane protein able to form oligomers through the coiled-coil structure. RCAS1 revealed different expression pattern from the known tumore associated antigens such as, YH206, GA733, CA125, CEA and sialyl Le molecules in human tumore cell lines. Recent study indicated RCAS1 acts as a ligand for a putative receptor present on various human cells including T, B and NK cells. RCAS1 inhibited the in vitro growth of receptor-expressing cells and induced apoptosis. It was suggested that tumore cells might evade immune surveillance by expression of RCAS1.

General readings

Ruder C, Reimer T, Delgado-Martinez I, Hermosilla R, Engelsberg A, Nehring R, Dorken B, Rehm A. EBAG9 adds a new layer of control on large dense-core vesicle exocytosis via interaction with Snapin. Mol Biol Cell. 2005 Mar;16(3):1245-57. Epub 2005 Jan 5. PMID: 15635093

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EBAG9 / RCAS1 (C-term) antibody

AP15755PU RCAS1 antibody stainng of Human Breast Carcinoma. Rabbit Purified Hu P
0.5 ml / €360.00
  Acris Antibodies GmbH

EBAG9 / RCAS1 (C-term) antibody

AP15755PU RCAS1 antibody stainng of Human Breast Carcinoma. Rabbit Purified Hu P
1 ml / €520.00
  Acris Antibodies GmbH

EBAG9 / RCAS1 (C-term) antibody

AP15755PU RCAS1 antibody stainng of Human Breast Carcinoma. Rabbit Purified Hu P
0.1 ml / €190.00
  Acris Antibodies GmbH

EBAG9 / RCAS1 (C-term) antibody

TA303119 (0.2µg/ml) staining of A431 lysate (35µg protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide.  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EBAG9 / RCAS1 antibody

WB Suggested Anti-EBAG9 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EBAG9 / RCAS1 antibody

IHC on human uterine cervical adenocarcinoma Mouse IgM 22-1-1 Azide Free Hu F, P
0.1 ml / €410.00
  Acris Antibodies GmbH

EBAG9 / RCAS1 (240-251) antibody

Skeletal muscle: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Aff - Purified Ajellomyces, Bear, Bov, Bt, Can, Chk, Elephant, Eq, Fi, Hu, L, Mky, Ms, Op, Por, Prim, Rb, Rt, Tr, Xen E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

EBAG9 / RCAS1 antibody

EBAG9 monoclonal antibody (M04), clone 4A10. Western Blot analysis of EBAG9 expression in MCF-7 ( Cat # L046V1 ). Mouse IgG2a 4A10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EBAG9 / RCAS1 antibody

EBAG9 polyclonal antibody ( Cat # PAB7053 ) ( 0.2 µg/ml ) staining of A-431 lysate ( 35 µg protein in RIPA buffer ) with ( B ) and without ( A ) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

EBAG9 / RCAS1 antibody

Immunohistochemical staining of EBAG9 on formalin fixed, paraffin embedded human breast cancer with EBAG9 polyclonal antibody. Rabbit Hu P
0.1 ml / €390.00
  Abnova Taiwan Corp.

EBAG9 / RCAS1 antibody

The tissue lysate derived from human liver tissue lysate was immunoprobed by EBAG9 polyclonal antibody ( Cat # PAB12726 ) at 1 : 500. An immunoreactive band is observed around ~32 kDa ( 1 ). The lane 2 is a negative control. Rabbit Bov, Hu, Ms, Rt C, E, IP, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

EBAG9 / RCAS1 (C-term) antibody

Human THP-1; WB Suggested Anti-EBAG9 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: THP-1 cell lysate; EBAG9 antibody - C-terminal region (ARP59868_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

EBAG9 / RCAS1 antibody

Western Blot: EBAG9/RCAS1 Antibody [NB100-41096] -  (0.2ug/ml) staining of A431 lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

EBAG9 / RCAS1 antibody

Western Blot: EBAG9/RCAS1  Antibody [NBP1-47292] - Detection of Human and Mouse EBAG9/RCAS1 by Western Blot (h&m) and Immunoprecipitation (h).  Samples:  Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-EBAG9/RCAS1 antibody was used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

EBAG9 / RCAS1 antibody

Immunohistochemistry-Paraffin: EBAG9 Antibody [NBP1-84922] - Immunohistochemical staining of human rectum shows strong cytoplasmic positivity, with a granular pattern, in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

EBAG9 / RCAS1 antibody

Immunohistochemistry-Paraffin: EBAG9 Antibody [NBP1-84923] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EBAG9 / RCAS1 (transcript variant 1)

EBAG9 / RCAS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EBAG9 / RCAS1 (transcript variant 2)

EBAG9 / RCAS1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EBAG9 / RCAS1 (28-213, His-tag)

EBAG9 / RCAS1 Human Purified > 90 % by SDS PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

EBAG9 / RCAS1 (28-213, His-tag)

EBAG9 / RCAS1 Human Purified > 90 % by SDS PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

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