ECSIT antibody

Principal name

ECSIT antibody

Alternative names for ECSIT antibody

SITPEC, Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, ECSIT homolog (Drosophila), Signaling intermediate in toll pathway evolutionarily conserved

SwissProt ID

Q3SX05 (Bovin), Q5XIC2 (Rat), Q9BQ95 (Human), Q9QZH6 (Mouse)

Gene ID

51295 (ECSIT), 26940 (Ecsit)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Ms (Mouse), Rt (Rat), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C)

Background of ECSIT antibody

Activation of NF-kappaB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses (reviewed in 1). Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88 (2) to kinases such as IRAK (3) and other intermediates such as TNF receptor associated factor (TRAF)-6 (4). ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway (5). Recently however, ECSIT has also been shown to be required for bone morphogenetic protein (Bmp) signaling and mesoderm formation during mouse embryogenesis (6), indicating the possibility of cross-talk between the TLR/ILB and Bmp signaling pathways.

General readings

Vogel, R.O., et al. Genes Dev. 21(5):615-624(2007).
Xiao, C., et al. Genes Dev. 17(23):2933-2949(2003).
Kopp, E., et al. Genes Dev. 13(16):2059-2071(1999).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ECSIT (C-term) antibody

Western blot analysis of ECSIT in human heart cell lysates with ECSIT antibody at (A) 0.5, (B) 1, and (C) 2 ug/ml. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ECSIT (C-term) antibody

ECSIT Antibody (C-term) (Cat. #TA324573) western blot analysis in Jurkat,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ECSIT antibody detected the ECSIT protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

ECSIT antibody

WB Suggested Anti-Ecsit Antibody Titration: 0.2-1 ug/ml; Positive Control: Mouse Heart Rabbit IgG Purified Ms WB
50 µg / €325.00
  OriGene Technologies, Inc.

ECSIT antibody

WB Suggested Anti-SITPEC Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:1562500; Positive Control: NIH/3T3 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ECSIT antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

ECSIT Control Peptide

ECSIT
50 µg / €160.00
  Acris Antibodies GmbH

ECSIT antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between SMURF1 and ECSIT. HeLa cells were stained with anti-SMURF1 rabbit purified polyclonal 1:1200 and anti-ECSIT mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 1B8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

ECSIT antibody

Immunohistochemistry using ECSIT polyclonal antibody ( Cat # PAB10005 ) shows staining of ECSIT in mouse heart tissue. Paraffin embedded formalin fixed tissue was reacted with primary antibody diluted 1 : 500. Hu, Ms
0.1 mg / €560.00
  Abnova Taiwan Corp.

ECSIT (N-term) antibody

Mouse Heart; WB Suggested Anti-Ecsit Antibody Titration: 0.2-1 ug/ml. Positive Control: Mouse Heart; Ecsit antibody - N-terminal region (ARP37175_P050) in Mouse Heart cells using Western Blot Rabbit Aff - Purified Ms WB
50 µg / €350.00
  AVIVA Systems Biology

ECSIT antibody

Mouse NIH-3T3; WB Suggested Anti-SITPEC Antibody Titration: 1.25ug/ml. ELISA Titer: 1:1562500. Positive Control: NIH/3T3 cell lysate; SITPEC antibody - middle region (ARP37176_T100) in Mouse NIH-3T3 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

ECSIT antibody

ECSIT Rabbit Purified Hu, Ms E, P, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

ECSIT antibody

Immunohistochemistry-Paraffin: IRAK2 Antibody [NBP1-77839] Immunohistochemistry using Rockland's IgG fraction of anti-IRAK-2 antibody shows staining of IRAK-2 in HeLa cells. The cells were fixed with paraformaldehyde and reacted with the primary antibody diluted to 1:500. Rabbit Aff - Purified Hu E, P, WB
0.1 mg / €480.00
  Novus Biologicals Inc.

ECSIT antibody

Immunohistochemistry-Paraffin: ECSIT Antibody [NBP1-91858] - Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ECSIT (transcript variant 3)

ECSIT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

ECSIT (19-217, His-tag)

ECSIT Human Purified > 90 % by SDS - PAGE E. coli
0.25 mg / €730.00
  Acris Antibodies GmbH

ECSIT (19-217, His-tag)

ECSIT Human Purified > 90 % by SDS - PAGE E. coli
50 µg / €300.00
  Acris Antibodies GmbH

ECSIT

ECSIT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ECSIT

ECSIT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

ECSIT

ECSIT Human Purified
10 µg / €380.00
  Abnova Taiwan Corp.

ECSIT

ECSIT Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

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