ECT2 antibody

Principal name

ECT2 antibody

Alternative names for ECT2 antibody

Epithelial cell-transforming sequence 2 oncogene

SwissProt ID

Q07139 (Mouse), Q9H8V3 (Human)

Gene ID

1894 (ECT2)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), Ze (Zebrafish), African clawed frog, Chimp (Chimpanzee), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP)

Background of ECT2 antibody

ECT2 is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of ECT2 is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Justilien, V., et al. Oncogene 28(41):3597-3607(2009).
Burkard, M.E., et al. PLoS Biol. 7 (5), E1000111 (2009).
Wolfe, B.A., et al. PLoS Biol. 7 (5), E1000110 (2009).
Hirata, D., et al. Clin. Cancer Res. 15(1):256-266(2009).
Seguin, L., et al. Mol. Cell. Biol. 29(2):570-581(2009).

20 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ECT2 antibody

WB Suggested Anti-ECT2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ECT2 antibody

WB Suggested Anti-ECT2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ECT2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECT2 (RC211319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECT2. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ECT2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ECT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807408) Mouse IgG1 OTI2D9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ECT2 pThr790 antibody

Western blot using affinity purified anti-ECT2 pT790 antibody shows detection of endogenous phosphorylated ECT2 (arrowhead) present in cell lysates from interphase (lane 1) and mitotic (lane 2) HeLa cells. Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA results using both phosphorylated and nonphosphorylated immunizing peptide. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000. Rabbit IgG Aff - Purified Can, Chimp, Chk, Hu, Ms, Rt, Ze E, IP, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

ECT2 (N-term) antibody

ECT2 Goat Aff - Purified Hu WB
0.1 mg / €340.00
  Acris Antibodies GmbH

ECT2 (Center) antibody

Western blot analysis of ECT2 Antibody Cat.-No AP51360PU-N in 293 cell line lysates (35ug/lane). This demonstrates the ECT2 antibody detected the ECT2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

ECT2 antibody

ECT2 Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ECT2 antibody

Western blot using ECT2 (phospho T790) polyclonal antibody ( Cat # PAB10006 ) showsdetection of endogenous phospho-ECT2 ( arrowhead ) present in cell lysates from interphase ( lane 1 ) and mitotic ( lane 2 ) HeLa cells.
Despite specific staining of interphase cells, this reagent is believed to be phospho specific based on ELISA resultsusing both phosphorylated and non-phosphorylated immunizing peptide.
After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 1,000.
Personal Communication, Toru Miki,CCR-NCI, Bethesda, MD. Rabbit Chimp, Chk, Hu, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

ECT2 antibody

Human HeLa; WB Suggested Anti-ECT2 Antibody Titration: 0.2-1 ug/ml. Positive Control: Hela cell lysate; ECT2 antibody - middle region (ARP52381_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ECT2 antibody

Human HepG2; WB Suggested Anti-ECT2 Antibody Titration: 0.2-1 ug/ml. Positive Control: HepG2 cell lysate; ECT2 antibody - middle region (ARP52382_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

ECT2 antibody

ELISA: ECT2 Antibody [36130002] Rabbit Hu E, WB
50 µg / €400.00
  Novus Biologicals Inc.

ECT2 antibody

ECT2 Goat IgG Aff - Purified Hu, Ms E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ECT2 antibody

ECT2 Antibody [NBP1-28729] - Detection of Human ECT2 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 mcg) from HeLa cells.   Antibody: Affinity purified rabbit anti-ECT2 antibody NBP1-28729 used for WB at 0.1 mcg/ml.  Detection: Chemiluminescence with an exposure time of 3 minutes. Rabbit IgG Aff - Purified Hu WB
0.1 mg / €440.00
  Novus Biologicals Inc.

ECT2 antibody

Western Blot: ECT2 Antibody [NBP1-58303] - This Anti-ECT2 antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

ECT2 antibody

Western Blot: ECT2 Antibody [NBP1-58321] - This Anti-ECT2 antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms WB
50 µg / €440.00
  Novus Biologicals Inc.

ECT2 antibody

Western Blot: ECT2 Antibody [NBP1-77940]  affinity purified anti-MECT2 antibody shows detection of MECT2 (lower arrowhead) and MECT2:MAML2 fusion protein (top arrowhead) in cell lysates. Lane 1 contains lysate from cells expressing MECT2 only. Lane 2 contains lysate from cells transfected with fusion protein. Lane 3 contains lysate from cells strongly expressing MECT2 and weakly expressing the fusion protein. Lane 4 contains lysate from control cells. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1,000 Rabbit Aff - Purified Bov, Hu, Ms, Rt, Ze E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ECT2

ECT2 Human
10 µg / €380.00
  Abnova Taiwan Corp.

ECT2

ECT2 Human
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ECT2 overexpression lysate

ECT2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

20 Item(s)

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