ECT2 antibody

Principal name

ECT2 antibody

Alternative names for ECT2 antibody

Epithelial cell-transforming sequence 2 oncogene

SwissProt ID

Q07139 (Mouse), Q9H8V3 (Human)

Gene ID

1894 (ECT2)

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Chimp (Chimpanzee), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Paraffin Sections (P)

Background of ECT2 antibody

ECT2 is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of ECT2 is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Justilien, V., et al. Oncogene 28(41):3597-3607(2009).
Burkard, M.E., et al. PLoS Biol. 7 (5), E1000111 (2009).
Wolfe, B.A., et al. PLoS Biol. 7 (5), E1000110 (2009).
Hirata, D., et al. Clin. Cancer Res. 15(1):256-266(2009).
Seguin, L., et al. Mol. Cell. Biol. 29(2):570-581(2009).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ECT2 antibody

  Rabbit polyclonal ECT2 phospho T790 antibody (Phospho-specific)  
ECT2 Rabbit IgG Purified Can, Chimp, Chk, Hu, Ms, Rt, Ze E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

ECT2 antibody

  Rabbit Polyclonal Anti-ECT2 Antibody  
WB Suggested Anti-ECT2 Antibody Titration: 0.2-1 ug/ml; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ECT2 antibody

  Rabbit Polyclonal Anti-ECT2 Antibody  
WB Suggested Anti-ECT2 Antibody Titration: 0.2-1 ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ECT2 antibody

  Rabbit Polyclonal ECT2 Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECT2 (RC211319, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECT2. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

ECT2 antibody

  ECT2 mouse monoclonal antibody, clone OTI2D9 (formerly 2D9)  
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-ECT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807408) Mouse IgG1 OTI2D9 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

ECT2 (N-term) antibody

ECT2 Goat Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

ECT2 (Center) antibody

Western blot analysis of ECT2 Antibody Cat.-No AP51360PU-N in 293 cell line lysates (35ug/lane). This demonstrates the ECT2 antibody detected the ECT2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

ECT2 - human gene knockout kit via CRISPR

ECT2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ECT2 - human gene knockout kit via CRISPR

ECT2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ECT2 - human gene knockout kit via CRISPR

ECT2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

ECT2 - human gene knockout kit via CRISPR

ECT2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ECT2 overexpression lysate

ECT2 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

12 Item(s)

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