EDF-1 antibody

Principal name

EDF-1 antibody

Alternative names for EDF-1 antibody

MBF1, EDF1, Endothelial differentiation-related factor 1, Multiprotein-bridging factor 1

SwissProt ID

O60869 (Human), P69736 (Rat), Q3T0V7 (Bovin), Q9JMG1 (Mouse)

Gene ID

8721 (EDF1)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Ze (Zebrafish), GP (Guinea Pig), Eq (Equine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of EDF-1 antibody

EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptiol machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptiol coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two altertively spliced transcripts which encode distinct proteins have been found for this gene. This gene encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptiol machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptiol coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two altertively spliced transcripts which encode distinct proteins have been found for this gene.

General readings

Brendel,C., et al., (2002) Mol. Endocrinol. 16 (6), 1367-1377

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EDF-1 antibody

EDF1 antibody (0.3µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EDF-1 antibody

WB Suggested Anti-EDF1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EDF-1 antibody

WB Suggested Anti-EDF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EDF-1 antibody

Host: Rabbit; Target Name: EDF1; Sample Tissue: Uterus Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EDF-1 antibody

WB Suggested Anti-EDF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EDF-1 (2-14) antibody

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bov, Can, Chk, Fi, Frog, Hu, Insect, Mky, Ms, Op, Por, Prim, Rt, Trout P
50 µg / €470.00
  Acris Antibodies GmbH

EDF-1 antibody

Western Blot analysis of EDF1 expression in transfected 293T cell line (H00008721-T01) by EDF1 MaxPab polyclonal antibody.

Lane1:EDF1 transfected lysate(16.39 KDa).
Lane 2:Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

EDF-1 antibody

Immunofluorescence of purified MaxPab antibody to EDF1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

EDF-1 antibody

EDF1 monoclonal antibody (M03), clone 3E6. Western Blot analysis of EDF1 expression in Hela NE(Cat # L013V3 ). Mouse IgG2a 3E6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EDF-1 antibody

human fibroblasts; WB Suggested Anti-GPD1 Antibody . . Titration: 5 ug/ml. . Positive Control: HG; EDF1 antibody - N-terminal region (ARP35695_P050) in human fibroblasts cells using Immunofluorescence Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

EDF-1 antibody

Human Jurkat; WB Suggested Anti-EDF1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:312500. Positive Control: Jurkat cell lysate; EDF1 antibody - N-terminal region (ARP37729_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

EDF-1 antibody

Human Prostate; Immunohistochemistry with Prostate tissue at an antibody concentration of 5ug/ml using anti-EDF1 antibody (ARP38383_P050); EDF1 antibody - middle region (ARP38383_P050) in Human Prostate cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

EDF-1 antibody

Immunohistochemistry-Paraffin: EDF1 Antibody [NBP1-84011] - Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity in squamous epithelial cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EDF-1 (transcript variant alpha)

EDF-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EDF-1 (transcript variant beta)

EDF-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EDF-1 (transcript variant alpha)

EDF-1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

EDF-1 (1-148, His-tag)

EDF-1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

EDF-1 (1-148, His-tag)

EDF-1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

EDF-1

EDF-1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

EDF-1

EDF-1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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