EDF-1 antibody

Principal name

EDF-1 antibody

Alternative names for EDF-1 antibody

MBF1, EDF1, Endothelial differentiation-related factor 1, Multiprotein-bridging factor 1

SwissProt ID

O60869 (Human), P69736 (Rat), Q3T0V7 (Bovin), Q9JMG1 (Mouse)

Gene ID

8721 (EDF1), 59022 (Edf1), 296570

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), African clawed frog, Ze (Zebrafish), Fi (Fish), Frog, Insect, Mky (Monkey), Op (Opossum), Prim (Primate), Trout, GP (Guinea Pig), Eq (Equine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P)

Background of EDF-1 antibody

EDF1 encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptiol machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptiol coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two altertively spliced transcripts which encode distinct proteins have been found for this gene. This gene encodes a protein that may regulate endothelial cell differentiation. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptiol machinery, thereby modulating the transcription of genes involved in endothelial differentiation. This protein has also been found to act as a transcriptiol coactivator by interconnecting the general transcription factor TATA element-binding protein (TBP) and gene-specific activators. Two altertively spliced transcripts which encode distinct proteins have been found for this gene.

General readings

Brendel,C., et al., (2002) Mol. Endocrinol. 16 (6), 1367-1377

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EDF-1 antibody

  Goat Polyclonal Anti-EDF1 Antibody  
EDF1 antibody (0.3µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EDF-1 antibody

  Rabbit polyclonal Anti-EDF1 Antibody  
WB Suggested Anti-EDF1 Antibody Titration: 1.25ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EDF-1 antibody

  Rabbit Polyclonal Anti-EDF1 Antibody  
WB Suggested Anti-EDF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt, Ze ICC/IF, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EDF-1 antibody

  Rabbit Polyclonal Anti-EDF1 Antibody  
Host: Rabbit; Target Name: EDF1; Sample Tissue: Uterus Tumor lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EDF-1 antibody

  Rabbit Polyclonal Anti-EDF1 Antibody  
WB Suggested Anti-EDF1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rt ICC/IF, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EDF-1 (2-14) antibody

Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat IgG Aff - Purified Bov, Can, Chk, Hu, Mky, Ms, Por, Rt, Xen P
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

EDF1 - human gene knockout kit via CRISPR

EDF1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EDF1 - human gene knockout kit via CRISPR

EDF1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EDF1 - human gene knockout kit via CRISPR

EDF1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EDF1 - human gene knockout kit via CRISPR

EDF1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Edf1 - mouse gene knockout kit via CRISPR

Edf1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Edf1 - mouse gene knockout kit via CRISPR

Edf1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Edf1 - mouse gene knockout kit via CRISPR

Edf1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Edf1 - mouse gene knockout kit via CRISPR

Edf1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EDF-1 (transcript variant alpha)

  Recombinant protein of human endothelial differentiation-related factor 1 (EDF1), transcript variant alpha  
EDF-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EDF-1 (transcript variant beta)

  Purified recombinant protein of Homo sapiens endothelial differentiation-related factor 1 (EDF1), transcript variant beta  
EDF-1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EDF-1 (transcript variant alpha)

  Recombinant protein of human endothelial differentiation-related factor 1 (EDF1), transcript variant alpha  
EDF-1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

EDF-1 (1-148, His-tag)

EDF-1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €750.00
  Acris Antibodies GmbH

EDF-1 (1-148, His-tag)

EDF-1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

EDF1 overexpression lysate

EDF1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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