EDG1 / S1P1 antibody

Principal name

EDG1 / S1P1 antibody

Alternative names for EDG1 / S1P1 antibody

S1P receptor 1, S1PR1, CHEDG1, EDG1, ECGF1, D1S3362, FLJ58121, Sphingosine 1-phosphate receptor 1, Endothelial differentiation G-protein coupled receptor 1

SwissProt ID

O08530 (Mouse), P21453 (Human), P48303 (Rat), Q5E9P3 (Bovin)

Gene ID

1901 (S1PR1)

Available reactivities

Hu (Human), Bov (Bovine), Eq (Equine), Fe (Feline), Hst (Hamster), Mky (Monkey), Ms (Mouse), Por (Porcine), Rt (Rat), Shrew, Bear, Bt (Bat), Can (Canine), Op (Opossum), Xen (Xenopus), Ze (Zebrafish), Tr (Turkey), L (Lizard), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Flow Cytometry (F), Frozen Sections (C)

Background of EDG1 / S1P1 antibody

EBP 50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP 50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes. EBP 50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP 50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP 50 is found exclusively at the apical membranes of epithelial cells.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EDG1 / S1P1 antibody

Western blot analysis of S1P1 in mouse thymus lysate with S1P1 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

Western blot analysis of lysates of RH7777 cells transfected with full lenth human EDG-1 protein using anti-EDG-1 CT antibody at 10 µg/ml (1), antibody preincubated with specific blocking peptide (2) and antibody preincubated with non-specific control peptide (3) using Pierce Femto Signal substrate. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €570.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

Figure 3. Immunofluorescence detection of S1P1 receptors in transfected cells and HUVEC.Endogenous S1P1 receptors were detected by confocal microscopy in HUVEC and in transfected CHO cells stably expressing S1P1-GFP with an anti-S1P1 monoclonal antibody (1/100, w/v).CHO-K1 cells were used as negative control. S1P1-GFP receptors were detected by fluorescence (excitation 488 nm, emission 500-550nm) (a, b, i, j, green fluorescence) and immunofluorescence (Cy3-Ac II anti-mouse, excitation 559 nm, emission 570-625 nm) with ananti-S1P1 monoclonal antibody (c, d, k, l, red fluorescence). Nuclei were stained with TO-PRO-3 (excitation 635 nm, emission 655-755nm, e, f, m, and n blue fluorescence). Merge images are presented (g, h, o, p).HUVEC were stained with anti-S1P1 primary antibody and Alexa-488 secondary antibody (excitation 488 nm, emission 500-550 nm).HUVEC control was realized with omitting anti-S1P1 primary antibody. Mouse IgG2a 2B9 Purified Hu F, ICC/IF, IP, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

Anti-S1PR1 / EDG1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rabbit Purified Hu E, P
50 µg / €345.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

Predicted band size: 43 kDa. Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

Predicted band size: 43 kDa. Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

Western blot analysis of RAEC cells (lanes 1 and 3) and in A-10 (lane 2 and 4) cell lysates: 1, 2. Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody (1:200). 3, 4. Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

Western Blot: EDG1 Antibody [TA337019] - Analysis of EDG-1 using EDG-1 polyclonal antibody. Human lung lysate in the 1) absence and 2) presence of immunizing peptide probed using EDG-1 antibody at 2 ug/ml. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EDG1 / S1P1 (C-term) antibody

Anti-S1PR1 / EDG1 antibody IHC of human heart, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Elephant, Eq, Fe, Hst, Hu, Mky, Ms, Por, Rt, Shrew P
50 µg / €379.00
  OriGene Technologies, Inc.

EDG1 / S1P1 (1st Cytopl. Dom.) antibody

Human, Placenta: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Fe, Hst, Hu, L, Mky, Ms, Op, Por, Rt, Tr, Xen, Ze P
50 µg / €379.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

Western blot (WB) analysis of EDG-1 antibody (Cat.-No.: AP01197PU-N) in extracts from COLO205 cells. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

Western blot (WB) analysis of EDG-1 antibody (Cat.-No.: AP01462PU-N) in extracts from HT-29 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG1 / S1P1 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

EDG1 / S1P1 Control Peptide

EDG1 / S1P1
50 µg / €160.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

Human Prostate, Endothelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

EDG1 MaxPab rabbit polyclonal antibody. Western Blot analysis of EDG1 expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

EDG1 monoclonal antibody (M01), clone 2E12 Western Blot analysis of EDG1 expression in Jurkat ( Cat # L017V1 ). Mouse IgG1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

Western Blot analysis of EDG1 expression in transfected 293T cell line by EDG1 monoclonal antibody (M03), clone 1F11.

Lane 1: EDG1 transfected lysate(42.8 KDa).
Lane 2: Non-transfected lysate. Mouse IgG3 1F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with S1PR1 polyclonal antibody ( Cat # PAB4718 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

Western blot analysis of lysates of McA-RH7777 cells transfected with full length human S1PR1 protein using S1PR1 polyclonal antibody ( Cat # PAB10360 ) at 10 µg/ml (lane 1), antibody preincubated with specific blocking peptide (lane 2) and antibody preincubated with non-specific control peptide (lane 3). Rabbit Hu, Ms, Rt WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

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