EDG1 / S1P1 antibody

Principal name

EDG1 / S1P1 antibody

Alternative names for EDG1 / S1P1 antibody

S1P receptor 1, S1PR1, CHEDG1, EDG1, ECGF1, D1S3362, FLJ58121, Sphingosine 1-phosphate receptor 1, Endothelial differentiation G-protein coupled receptor 1

SwissProt ID

O08530 (Mouse), P21453 (Human), P48303 (Rat), Q5E9P3 (Bovin)

Gene ID

1901 (S1PR1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Rb (Rabbit), Eq (Equine), Fe (Feline), Hst (Hamster), Shrew, Bear, Bt (Bat), Op (Opossum), Xen (Xenopus), Ze (Zebrafish), Tr (Turkey), L (Lizard)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Immunoprecipitation (IP)

Background of EDG1 / S1P1 antibody

The EDG (endothelial differentiation gene) family of G protein coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. EDG-6, which is expressed in lymphoid and hematopoietic tissues and in lung, signals through Gi/o proteins, which activate growth related pathways.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EDG1 / S1P1 antibody

  Rabbit Polyclonal S1P1 Antibody  
Western blot analysis of S1P1 in mouse thymus lysate with S1P1 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

Western blot analysis of lysates of RH7777 cells transfected with full lenth human EDG-1 protein using anti-EDG-1 CT antibody at 10 µg/ml (1), antibody preincubated with specific blocking peptide (2) and antibody preincubated with non-specific control peptide (3) using Pierce Femto Signal substrate. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €590.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

Figure 3. Immunofluorescence detection of S1P1 receptors in transfected cells and HUVEC.Endogenous S1P1 receptors were detected by confocal microscopy in HUVEC and in transfected CHO cells stably expressing S1P1-GFP with an anti-S1P1 monoclonal antibody (1/100, w/v).CHO-K1 cells were used as negative control. S1P1-GFP receptors were detected by fluorescence (excitation 488 nm, emission 500-550nm) (a, b, i, j, green fluorescence) and immunofluorescence (Cy3-Ac II anti-mouse, excitation 559 nm, emission 570-625 nm) with ananti-S1P1 monoclonal antibody (c, d, k, l, red fluorescence). Nuclei were stained with TO-PRO-3 (excitation 635 nm, emission 655-755nm, e, f, m, and n blue fluorescence). Merge images are presented (g, h, o, p).HUVEC were stained with anti-S1P1 primary antibody and Alexa-488 secondary antibody (excitation 488 nm, emission 500-550 nm).HUVEC control was realized with omitting anti-S1P1 primary antibody. Mouse IgG2a 2B9 Purified Hu F, ICC/IF, IP, WB
0.1 mg / €300.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

  Rabbit polyclonal anti-S1PR1/EDG1 antibody  
Western blot analysis of extracts from COLO205 cells, using EDG1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

  EDG1 / S1P1 Rabbit Polyclonal (aa25-40) Antibody  
Anti-S1PR1 / EDG1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rabbit Purified Hu E, P
50 µg / €345.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

  Anti-S1PR1 Rabbit Polyclonal Antibody  
Predicted band size: 43 kDa. Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

  Anti-S1PR1 Rabbit Polyclonal Antibody  
Predicted band size: 43 kDa. Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

  Rabbit Polyclonal Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular)  
Western blot analysis of RAEC cells (lanes 1 and 3) and in A-10 (lane 2 and 4) cell lysates: 1, 2. Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody (1:200). 3, 4. Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen. Rabbit Purified Hu, Ms, Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

  Rabbit Polyclonal S1P1/EDG-1 Antibody  
Western Blot: EDG1 Antibody [TA337019] - Analysis of EDG-1 using EDG-1 polyclonal antibody. Human lung lysate in the 1) absence and 2) presence of immunizing peptide probed using EDG-1 antibody at 2 ug/ml. Rabbit Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EDG1 / S1P1 (C-term) antibody

  Rabbit Polyclonal Anti-S1PR1 Antibody (C-Terminus)  
Anti-S1PR1 / EDG1 antibody IHC of human heart, vessel. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Elephant, Eq, Fe, Hst, Hu, Mky, Ms, Por, Rt, Shrew P
50 µg / €379.00
  OriGene Technologies, Inc.

EDG1 / S1P1 (1st Cytopl. Dom.) antibody

  Rabbit Polyclonal Anti-S1PR1 Antibody (Cytoplasmic Domain)  
Human, Placenta: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Fe, Hst, Hu, L, Mky, Ms, Op, Por, Rt, Tr, Xen, Ze P
50 µg / €379.00
  OriGene Technologies, Inc.

EDG1 / S1P1 antibody

Western blot (WB) analysis of EDG-1 antibody (Cat.-No.: AP01197PU-N) in extracts from COLO205 cells. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

Western blot (WB) analysis of EDG-1 antibody (Cat.-No.: AP01462PU-N) in extracts from HT-29 cells. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG1 / S1P1 (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

Human Prostate, Endothelium: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µl / €470.00
  Acris Antibodies GmbH

EDG1 / S1P1 antibody

EDG1 MaxPab rabbit polyclonal antibody. Western Blot analysis of EDG1 expression in mouse lung. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

EDG1 monoclonal antibody (M01), clone 2E12 Western Blot analysis of EDG1 expression in Jurkat ( Cat # L017V1 ). Mouse IgG1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

Western Blot analysis of EDG1 expression in transfected 293T cell line by EDG1 monoclonal antibody (M03), clone 1F11.

Lane 1: EDG1 transfected lysate(42.8 KDa).
Lane 2: Non-transfected lysate. Mouse IgG3 1F11 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with S1PR1 polyclonal antibody ( Cat # PAB4718 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma. Rabbit Hu, Ms E, P
0.4 ml / €390.00
  Abnova Taiwan Corp.

EDG1 / S1P1 antibody

Western blot analysis of lysates of McA-RH7777 cells transfected with full length human S1PR1 protein using S1PR1 polyclonal antibody ( Cat # PAB10360 ) at 10 µg/ml (lane 1), antibody preincubated with specific blocking peptide (lane 2) and antibody preincubated with non-specific control peptide (lane 3). Rabbit Hu, Ms, Rt WB
0.1 mg / €640.00
  Abnova Taiwan Corp.

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