EDG5 / S1P2 antibody

Principal name

EDG5 / S1P2 antibody

Alternative names for EDG5 / S1P2 antibody

S1P receptor 2, S1PR2, EDG5, H218, LPB2, AGR16, Gpcr13, Sphingosine 1-phosphate receptor 2, Endothelial differentiation G-protein coupled receptor 5

SwissProt ID

O95136 (Human), P47752 (Rat), P52592 (Mouse)

Gene ID

9294 (S1PR2), 14739 (S1pr2), 29415

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of EDG5 / S1P2 antibody

The EDG (endothelial differentiation gene) family of G protein coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high-affinity. EDG-6 is a low-affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain. Expressed in lymphoid and hematopoietic tissues and in lung, EDG-6 signals through Gi/o proteins, which activate growth related pathways.

General readings

1. McGeachie M, Ramoni RL, Mychaleckyj JC, Furie KL, Dreyfuss JM, Liu Y, Herrington D, Guo X, Lima JA, Post W, Rotter JI, Rich S, Sale M, Ramoni MF, Integrative predictive model of coronary artery calcification in atherosclerosis. Circulation 2009 Dec 120 (24): 2448-54.
PMID: 19948975

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EDG5 / S1P2 antibody

Western blot of HeLa (lane 1) and PC12 cells (lane 2) using anti EDG5 antibody Cat.-No AP01198PU-N at 1/500 dilution. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG5 / S1P2 antibody

Western blo (WB) analysis of EDG-5 antibody Cat.-No AP01311PU-N) at 1/500 dilution:
Lane 1: HEK293T whole cell lysate.
Lane 2: Raw264.7 whole cell lysate.
Lane 3: H9C2 whole cell lysate. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG5 / S1P2 antibody

  Goat Anti-S1PR2 Antibody  
(0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EDG5 / S1P2 antibody

  Rabbit polyclonal anti-EDG5 antibody  
Western blot analysis of extracts from COLO205 cells, using EDG5 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG5 / S1P2 antibody

  Rabbit polyclonal Anti-Sphingosine 1-Phosphate Receptor 2  
Western blot analysis of rat kidney (lanes 1 and 3) and rat heart (lanes 2 and 4) lysates: 1, 2. Anti-Sphingosine 1-Phosphate Receptor 2 antibody, (1:200). 3, 4. Anti-Sphingosine 1-Phosphate Receptor 2 antibody, preincubated with the control peptide antigen. Rabbit Purified Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.

EDG5 / S1P2 antibody

  Rabbit Polyclonal Anti-S1PR2 Antibody  
Host: Rabbit; Target Name: S1PR2; Sample Tissue: COLO205 Whole Cell lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

EDG5 / S1P2 antibody

  Rabbit Polyclonal Anti-S1PR2 Antibody  
Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EDG5 / S1P2 antibody

  Rabbit Polyclonal Anti-S1PR2 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using (S1PR2 Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) Rabbit IgG Purified Hu E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG5 / S1P2 (Cytopl. Dom) antibody

Cells expressing target protein - immunostained with target antibody Rabbit Aff - Purified Hu ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

EDG5 / S1P2 (C-term) antibody

Cells expressing target protein - immunostained with target antibody Rabbit Aff - Purified Hu E, ICC/IF, P
50 µg / €470.00
  Acris Antibodies GmbH

EDG5 / S1P2 antibody

Western blot analysis of extracts from COLO205 cells, using EDG5 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

EDG5 / S1P2 antibody

Western blot analysis of extracts from COLO205 cells, using EDG5 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

EDG5 / S1P2 antibody

Western blot analysis of extracts from HT-29 cells, using EDG5 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

EDG5 / S1P2 antibody

Western blot analysis of extracts from HT-29 cells, using EDG5 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

S1PR2 - human gene knockout kit via CRISPR

S1PR2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

S1PR2 - human gene knockout kit via CRISPR

S1PR2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

S1pr2 - mouse gene knockout kit via CRISPR

S1pr2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

S1pr2 - mouse gene knockout kit via CRISPR

S1pr2 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

S1pr2 - mouse gene knockout kit via CRISPR

S1pr2 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

S1pr2 - mouse gene knockout kit via CRISPR

S1pr2 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

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