EDG7 / LPAR3 antibody

Principal name

EDG7 / LPAR3 antibody

Alternative names for EDG7 / LPAR3 antibody

EDG7, LPAR3, LPA-3, HOFNH3O, GPCR, Endothelial differentiation, lysophosphatidic acid G-protein coupled receptor 7, Lysophosphatidic acid receptor 3, Lysophosphatidic acid receptor Edg-7

SwissProt ID

Q8K5E0 (Rat), Q9EQ31 (Mouse), Q9UBY5 (Human)

Gene ID

23566 (LPAR3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bt (Bat), Bov (Bovine), Can (Canine), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Bear, Chk (Chicken), Op (Opossum), Xen (Xenopus), Tr (Turkey)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of EDG7 / LPAR3 antibody

The EDG (endothelial differentiation gene) family of G protein-coupled receptors consists of eight family members that bind lysophospholipid (LPL) mediators, including sphingosine-1-phosphate (SPP) and lysophosphatidic acid (LPA). EDG-1, EDG-3, EDG-5 (also designated H218 and AGR16) and EDG-8 bind SPP with high affinity. EDG-6 is a low affinity receptor for SPP. LPA preferentially binds to EDG-2, EDG-4 and EDG-7. The EDG receptors couple to multiple G proteins to signal through Ras, MAP kinase, Rho, Phospholipase C or other tyrosine kinases, which lead to cell survival, growth, migration and differentiation. EDG-1 signals through Gi proteins to activate Akt and is expressed in glioma cells. EDG-2 is expressed in brain, especially in white matter tract regions, while EDG-3 is expressed in cardiovascular tissue and in cerebellum. EDG-4 is highly expressed on leukocytes and brain, and EDG-5 has wide tissue distribution, including cardiovascular tissue and brain.
EDG7, a Lysophospholipid/Lysosphingolipid Receptor, was identified by RT-PCR from Jurkat T-cells. It is activated by unsaturated lysophosphatidic acid LPA.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EDG7 / LPAR3 (2nd cytopl. dom.) antibody

Anti-EDG7 antibody IHC of human brain, amygdala. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Eq, Hst, Hu, Mky, Ms, Op, Rt, Tr P
50 µg / €379.00
  OriGene Technologies, Inc.

EDG7 / LPAR3 (3rd Cytoplasm. Dom.) antibody

Anti-LPAR3 / LPA3 / EDG7 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Bt, Can, Chk, Eq, Hst, Hu, Mky, Ms, Op, Platypus, Por, Rt, Tr, Xen P
50 µg / €379.00
  OriGene Technologies, Inc.

EDG7 / LPAR3 antibody

Western blot analysis of extracts from Jurkat cells, using EDG7 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG7 / LPAR3 antibody

Western blot analysis of extracts from Jurkat cells, using EDG7 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG7 / LPAR3 antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and liver cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using LPAR3 antibody at dilution 1/10, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG7 / LPAR3 antibody

Predicted cell location: Cytoplasm. Positive control: Human brain and liver cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human brain tissue using LPAR3 antibody at dilution 1/40, on the right is treated with the synthetic peptide. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EDG7 / LPAR3 antibody

Western blot (WB) analysis of EDG-7 antibody (Cat.-No.: AP01256PU-N) at 1/500 dilution Lane1:Hela cell lysate Lane2:Raw264.7 cell lysate Lane3:H9C2 cell lysate Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG7 / LPAR3 antibody

Western blot (WB) analysis of EDG-7 antibody (Cat.-No.: AP01312PU-N)  in extracts from Jurkat cells. Rabbit Aff - Purified Hu E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EDG7 / LPAR3 (1-12) antibody

AP07446PU-N EDG7 antibody staining of Formalin-Fixed Paraffin-Embedded Human Heart at 5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €470.00
  Acris Antibodies GmbH

EDG7 / LPAR3 (Internal) antibody

Human Liver (formalin-fixed, paraffin-embedded) stained with LPAR3 antibody AP22831PU-N at 5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Bear, Bov, Bt, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Prim, Rt E, P
50 µg / €470.00
  Acris Antibodies GmbH

EDG7 / LPAR3 antibody

Western blot analysis using LPAR3 polyclonal antibody ( Cat # PAB10127 ) on McA-RH7777 cell lysates transfected with full length human LPAR3. Hu
0.1 mg / €640.00
  Abnova Taiwan Corp.

EDG7 / LPAR3 antibody

Immunofluorescence analysis of LOVO cells, using EDG7 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

EDG7 / LPAR3 antibody

Immunofluorescence analysis of LOVO cells, using EDG7 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

EDG7 / LPAR3 antibody

Immunofluorescence analysis of A549 cells, using EDG7 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

EDG7 / LPAR3 antibody

Immunofluorescence analysis of A549 cells, using EDG7 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

EDG7 / LPAR3 antibody

Immunohistochemical analysis of EDG7 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

EDG7 / LPAR3 antibody

Immunohistochemical analysis of EDG7 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

EDG7 / LPAR3 antibody

Immunohistochemical analysis of EDG7 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

EDG7 / LPAR3 antibody

Western blot analysis of EDG7 expression in MCF7 (A); PC3 (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

EDG7 / LPAR3 antibody

Western blot analysis of EDG7 expression in MCF7 (A); PC3 (B) whole cell lysates. Rabbit Aff - Purified Hu, Ms WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

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