EEF-2 kinase antibody

Principal name

EEF-2 kinase antibody

Alternative names for EEF-2 kinase antibody

Elongation factor 2 kinase, EEF2K, EF2K, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase

SwissProt ID

O00418 (Human), O08796 (Mouse), P70531 (Rat)

Gene ID

29904 (EEF2K)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of EEF-2 kinase antibody

The activity of the purified eukaryotic elongation-factor-2 kinase (eEF2K) is completely dependent on calcium and calmodulin, and autophosphorylation on serine and threonine residues is calcium/calmodulin-dependent. eEF2K is a ubiquitous protein kinase that phosphorylates and inactivates eEF2, and thus can modulate the rate of polypeptide chain elongation during translation. eEF2K is 120 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38g, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.

General readings

Ryazanov A.G., et al. (1997) Proc. Natl. Acad. Sci. U.S.A. 94:4884-4889Knebel A., et al. (2001) EMBO J. 20:4360-4369

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EEF-2 kinase antibody

Sample (50 ug of whole cell lysate). A: mouse liver. 7.5% SDS PAGE. TA308475 diluted at 1:1000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EEF-2 kinase antibody

Western blot analysis of extracts of various cell lines, using EEF2K antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

EEF-2 kinase antibody

Immunohistochemical analysis in paraffin-embedded human breast carcinoma tissue using EEF-2 kinase antibody (Cat.-No. AP06095PU-N). Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EEF-2 kinase antibody

Western blot (WB) analysis of eEF2K antibody (Cat.-No.: AP21133PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EEF-2 kinase antibody

EEF2K MaxPab polyclonal antibody. Western Blot analysis of EEF2K expression in human colon. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

EEF-2 kinase pSer366 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2K (Phospho-Ser366) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

EEF-2 kinase pSer366 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2K (Phospho-Ser366) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

EEF-2 kinase antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using eEF2K (Ab-366) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

EEF-2 kinase antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using eEF2K (Ab-366) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

EEF-2 kinase antibody

Western blot analysis of extracts from Jurkat cells, using eEF2K (Ab-359) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

EEF-2 kinase antibody

Western blot analysis of extracts from Jurkat cells, using eEF2K (Ab-359) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

EEF-2 kinase pSer366 antibody

Immunohistochemical analysis of EEF2K (pS366) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Rt P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

EEF-2 kinase pSer366 antibody

Immunohistochemical analysis of EEF2K (pS366) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Rt P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

EEF-2 kinase pSer366 antibody

Immunohistochemical analysis of EEF2K (pS366) staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Rt P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

EEF-2 kinase antibody

Immunohistochemical analysis of EEF2K staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Rt P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

EEF-2 kinase antibody

Immunohistochemical analysis of EEF2K staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Rt P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

EEF-2 kinase antibody

Immunohistochemical analysis of EEF2K staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Mky, Rt P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

EEF-2 kinase antibody

EEF-2 kinase Rabbit IgG Aff - Purified Hu, Rt P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

EEF-2 kinase antibody

Western Blot: EEF2K Antibody [NBP1-32673] - (30 ug of whole cell lysate) A: Hela S3 7.5% SDS PAGE diluted at 1:1000 Rabbit IgG Hu WB
0.1 mg / €420.00
  Novus Biologicals Inc.

EEF-2 kinase antibody

EEF-2 kinase Rabbit IgG Aff - Purified Hu, Rt E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

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