EEF2 antibody

Principal name

EEF2 antibody

Alternative names for EEF2 antibody

EF-2, EF2, Elongation factor 2

SwissProt ID

P05197 (Rat), P13639 (Human), P55823 (Rabit), P58252 (Mouse)

Gene ID

1938 (EEF2), 13629 (Eef2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Ce (C. elegans), Dros (Drosophila), Bov (Bovine), Chk (Chicken), Mky (Monkey), Ze (Zebrafish), Can (Canine), Eq (Equine), GP (Guinea Pig)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C)

Background of EEF2 antibody

Eukaryotic elongation factor 2 (eEF2) catalyzes the translocation of peptidyl-tR from the A site to the P site on the ribosome. It has been shown that phosphorylation of eEF2 at threonine 56 by eEF2 kise inhibits its activity. eEF2 kise is normally dependent on Ca2+ ions and calmodulin. eEF2 kise can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation. The ictive phosphorylated eEF2 can be converted to its active nonphosphorylated form by a protein phosphatase, most likely a form of protein phosphatase-2A (PP-2A). Insulin, which activates protein synthesis in a wide range of cell types, induces rapid dephosphorylation of eEF2 through mTOR sigling and may involve modulation of the activity of the PP-2A or the eEF2 kise or both.

General readings

1. Collodoro M  et al. Identification and quantification of concentration-dependent biomarkers in MCF-7/BOS cells exposed to 17ß-estradiol by 2-D DIGE and label-free proteomics. J Proteomics 75:4555-69 (2012). WB ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EEF2 antibody

Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and kidney cancer tissues (right) using EEF2 antibody Cat.-No AM06603SU-N with DAB staining. Mouse IgG1 5B6 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

EEF2 antibody

  EEF2 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines , using EEF2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

EEF2 antibody

  Rabbit Polyclonal Anti-EEF2 Antibody - N-terminal region  
WB Suggested Anti-EEF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Ms, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EEF2 antibody

  Mouse Monoclonal eEF2 Antibody  
Western blot detection of eEF2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s. Mouse IgG2b 4B3-G7-H5 Purified ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EEF2 antibody

  Rabbit Polyclonal Anti-EEF2 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-6: LoVo cells, HepG2 cells, NIH/3T3 cells, MCF7 cells, 293T cells, Hela cells, Primary antibody: (EEF2 Antibody) at dilution 1/200, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 20 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EEF2 antibody

Immunohistochemistry (IHC) analyzes of eEF2 antibody (Cat.-No.: AP06094PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EEF2 antibody

EEF2 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

EEF2 pThr56 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

EEF2 pThr56 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eEF2 (Phospho-Thr56) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

EEF2 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using eEF2 (Ab-56) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

EEF2 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using eEF2 (Ab-56) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €310.00
  Assay Biotech

EEF2 antibody

Human Small intestine; Small intestine; EEF2 antibody - N-terminal region (ARP58457_P050) in Human Small intestine cells using Immunohistochemistry Rabbit Aff - Purified Ce, Dros, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

EEF2 antibody

Immunohistochemical analysis of EEF2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Rt, Ze ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

EEF2 antibody

Immunohistochemical analysis of EEF2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Rt, Ze ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

EEF2 antibody

Immunohistochemical analysis of EEF2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Chk, Hu, Mky, Ms, Rt, Ze ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EEF2

  Recombinant protein of human eukaryotic translation elongation factor 2 (EEF2)  
EEF2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EEF2 (574-858, His-tag)

EEF2 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

EEF2 (574-858, His-tag)

EEF2 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

EEF2

EEF2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

EEF2

EEF2 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

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