EGF antibody

Principal name

EGF antibody

Alternative names for EGF antibody

Urogastrone, Epidermal growth factor, URG, HOMG4, Pro-epidermal growth factor

SwissProt ID

P01132 (Mouse), P01133 (Human), P07522 (Rat), Q00968 (Pig)

Gene ID

1950 (EGF), 13645 (Egf), 25313

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat, Rat, Sheep

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), Western blot / Immunoblot (WB), Paraffin Sections (P), Functional assay (FN), Neutralisation (NEUT), ELISA (capture) (E(capture)), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Flow Cytometry (F), Immunoprecipitation (IP)

Background of EGF antibody

Epidermal growth factor (EGF) is a single chain poly-peptide hormone (6 kDa) which was first isolated from the submandibular gland of the mouse and is a potent growth-promoting factor for a variety of tissue cells in vivo and in vitro. Human EGF (hEGF) has also been extracted from human urine and shown to be identical or closely related to -urogastrone, a potent inhibitor of gastric acid secretion. Human EGF is structurally homologous to human transforming growth factor-alpha (TGF-Alpha) which also exerts its actions through EGF receptors. It is homologous to a sequence contained in a 19 kDa protein of Vaccinia virus, which appears to utilize the EGF receptor to gain entry into cells. Cloning data for mouse and human EGF suggest that the 53 amino-acid EGF peptide is cleaved from a 125-130 kDa, 1217 amino-acid precursor containing an N-terminal 29 amino-acid signal sequence. Mouse and human EGF exhibit 70% similarity at the amino acid level and are highly species cross-reactive. Structures obtained for both mouse and human EGF suggest that the protein exists largely in a beta sheet conformation, with a C-terminal hairpin that may be involved in receptor binding. EGF acts through a specific cell surface receptor glycoprotein of approximately 170 kDa. EGF binding induces phosphatidylinositide hydrolysis and generation of diacylglycerol (DAG). DAG-dependent activation of protein kinase C, in turn, results in the phosphorylation of a threonine residue at position 654 of the receptor, in attenuation of the intrinsic tyrosine kinase activity, and subsequently alterations of receptor homeostasis. Cellular metabolic effects of EGF include stimulation of ion fluxes, glucose transport, glycolysis and synthesis of DNA, RNA and proteins. EGF acts on a variety of tissues and binding has been demonstrated in virtually all cell types tested, with the exception of those of the hematopoietic lineage. EGF is mitogenic for a variety of epidermal and epithelial cells, including fibroblasts, glial cells, mammary epithelial cells, vascular and corneal endothelial cells, bovine granulosa, rabbit chondrocytes, HeLa and SV40-3T3 cells. Human EGF is found in body fluids such as urine, milk, saliva, sweat, and seminal fluid and has its highest concentration in alpha granules of blood platelets. It has a widespread distribution in human tissues and organs, including neoplasms. The biological role of EGF includes the inhibition of gastric acid secretion, support of growth and differentiation during fetal development, neuromodulation in the central nervous system and stimulation of epidermal growth and keratinization. Since EGF plays a role in the proliferation and/ or differentiation of normal as well as tumor cells derived from a variety of tissues, 5 and can contribute to pathological states, an in vitro assay for its detection and quantification is desirable. Monoclonal antibody reacting specifically with EGF may be used in the determination and quantification of the molecule in many in vitro systems and in vivo human models, and for studies on structure-function relationship of this factor in binding to the receptor molecule.

General readings

General / background references:Deuel TF Polypeptide growth factors: roles in normal and abnormal cell growth. Annu Rev Cell Biol 3:443-92 (1987). PubMed PMID: 3318882
Eppstein DA et al. Epidermal growth factor receptor occupancy inhibits vaccinia virus infection. Nature 318:663-5 (1985). PubMed PMID: 3001528
Cohen S & Carpenter G Human epidermal growth factor: isolation and chemical and biological properties. Proc Natl Acad Sci U S A 72:1317-21 (1975). PubMed PMID: 1055407

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EGF antibody

Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using EGF antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGF antibody

Western blot analysis of Mouse liver tissue using TA324064(EGF Antibody) at dilution 1/500 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGF antibody

To detect hEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EGF antibody

To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

To detect mEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF. Goat IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EGF antibody

To detect mEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine EGF (TA328508) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEGF. Goat IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

To detect Rat EGF by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat EGF  as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF. Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

TA329069 (1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

WB Suggested Anti-EGF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

EGF antibody

Western blot analysis of EGF expression in NIH/3T3 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGF (RC210817, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGF. Rabbit IgG Purified Hu Dot, E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

Immunohistochemical analysis of paraffin-embedded human Uterus tissues using anFtEG mouse mAb Mouse IgG1 9D7F11 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

EGF antibody

Immunofluorescence analysis of NIH/3T3 cells using EGF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 4E11 Ascites Hu E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-21 Aff - Purified Hu E
0.5 mg / €470.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-120 Aff - Purified Hu E
0.5 mg / €470.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-145 Aff - Purified Hu E
0.5 mg / €470.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-146 Biotin Hu E, E(detection)
0.1 ml / €510.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-146 HRP Hu E
0.2 ml / €510.00
  Acris Antibodies GmbH

EGF antibody

Staining of methanol-fixed Hela cells by Immunocytochemistry using Human EGF Antibody (Clone S-146) at 1/100 (10 µg/ml). Mouse IgG1 S-146 Purified Hu E(detection), ICC/IF, WB
0.5 mg / €470.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-147 Aff - Purified Hu E
0.5 mg / €470.00
  Acris Antibodies GmbH

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