EGF antibody

Principal name

EGF antibody

Alternative names for EGF antibody

Urogastrone, Epidermal growth factor, URG, HOMG4, Pro-epidermal growth factor

SwissProt ID

P01132 (Mouse), P01133 (Human), P07522 (Rat), Q00968 (Pig)

Gene ID

1950 (EGF), 25313 (Egf), 13645

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Rat, Sheep, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Frozen Sections (C), ELISA (capture) (E(capture)), Paraffin Sections (P), Functional assay (FN), Neutralisation (NEUT), Flow Cytometry (F), Immunoprecipitation (IP), Dot blot (Dot)

Background of EGF antibody

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF., amphiregulin (AR), betacellulin (BTC), epiregulin(EPR), heparinbinding EGF-like growth factor (HB-EGF), epigen, and the neuregulins (NRG)1 through-6. Members of the EGF family share a structural motif, the EGF-like domain, which is characterized by three intramolecular disulfide bonds that are formed by six similarly spaced conserved cysteine residues . All EGF family members are synthesized as type I transmembrane precursor proteins that may contain several EGF domains in the extracellular region. The 1207 amino acid (aa) human EGF precursor contains nine EGF domains and nine LDLR class B repeats. The mature protein consists of 53 aa and is generated by proteolytic excision of the EGF domain proximal to the transmembrane region . EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid . Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members . These receptors undergo a complex pattern of ligand induced homoor heterodimerization to transducer EGF family signals . EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules . Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

General readings

Harris, R.C. et al. (2003) Exp. Cell Res. 284:2.

Carpenter, G. and S. Cohen, (1990) J. Biol. Chem. 265:7709.

Bell, G.I. et al. (1986) Nucl. Acids Res. 14:8427.

Carpenter, G. and Zendegui, J.G. (1986) Exp. Cell Res.164:1.

Jorissen, R.N. et al. (2003) Exp. Cell Res. 284:31.

Gamett, D.C. et al. (1997) J. Biol. Chem. 272:12052.

Qian, X. et al. (1994) Proc. Natl. Acad. Sci. 91:1500.

Qian, X. et al. (1999) J. Biol. Chem. 274:574.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EGF antibody

  Anti-EGF Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human cervical cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using EGF antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGF antibody

  Anti-EGF Rabbit Polyclonal Antibody  
Western blot analysis of Mouse liver tissue using TA324064(EGF Antibody) at dilution 1/500 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGF antibody

  Biotinylated Anti-Human EGF Rabbit Polyclonal Antibody  
To detect hEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EGF antibody

  Anti-Human EGF Rabbit Polyclonal Antibody  
To detect hEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human EGF (TA328291) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

  Biotinylated Anti-Murine EGF Goat Polyclonal Antibody  
To detect mEGF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mEGF. Goat IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EGF antibody

  Anti-Murine EGF Goat Polyclonal Antibody  
To detect mEGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine EGF (TA328508) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEGF. Goat IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

  Anti-Rat EGF Rabbit Polyclonal Antibody  
To detect Rat EGF by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat EGF  as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat EGF. Rabbit IgG Purified Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

  Pro-EGF Goat Polyclonal Antibody  
TA329069 (1µg/ml) staining of Human Prostate lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

  Rabbit Polyclonal Anti-EGF Antibody  
WB Suggested Anti-EGF Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 1562500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Can, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

EGF antibody

  Rabbit Polyclonal Anti-EGF Antibody  
Western blot analysis of EGF expression in NIH/3T3 cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

  Rabbit Polyclonal EGF Antibody  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGF (RC210817, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGF. Rabbit IgG Purified Hu Dot, E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGF antibody

Immunohistochemical analysis of paraffin-embedded human Uterus tissues using anFtEG mouse mAb Mouse IgG1 9D7F11 Ascites Hu E, P, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

EGF antibody

Immunofluorescence analysis of NIH/3T3 cells using EGF mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG1 4E11 Ascites Hu E, ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-21 Aff - Purified Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-120 Aff - Purified Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-145 Aff - Purified Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-146 Biotin Hu E, E(detection)
0.1 ml / €510.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-146 HRP Hu E
0.2 ml / €510.00
  Acris Antibodies GmbH

EGF antibody

Staining of methanol-fixed Hela cells by Immunocytochemistry using Human EGF Antibody (Clone S-146) at 1/100 (10 µg/ml). Mouse IgG1 S-146 Purified Hu E(detection), ICC/IF, WB
0.5 mg / €460.00
  Acris Antibodies GmbH

EGF antibody

EGF Mouse IgG1 S-147 Aff - Purified Hu E
0.5 mg / €460.00
  Acris Antibodies GmbH

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