EGLN1 / PHD2 antibody

Principal name

EGLN1 / PHD2 antibody

Alternative names for EGLN1 / PHD2 antibody

EGLN-1, C1orf12, HIF-PH2, HIF-prolyl hydroxylase 2, HPH-2, SM-20, Egl nine homolog 1, Hypoxia-inducible factor prolyl hydroxylase 2, Prolyl hydroxylase domain-containing protein 2

SwissProt ID

P59722 (Rat), Q91YE3 (Mouse), Q9GZT9 (Human)

Gene ID

54583 (EGLN1), 112405 (Egln1)

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of EGLN1 / PHD2 antibody

HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) that mediate important physiological responses to hypoxia by modulating HIF1alpha levels. The HIF-alpha is regulated by hydroxylation, both by a family of PHDs leading to ubiquitition and proteasomal degradation, and by transcriptiol ictivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). Under normoxic conditions, HIF Prolyl Hydroxylase 2 (HIF1-PH; also called PHD1 or Egln2) catalyzes the post-translatiol formation of 4-hydroxyproline through hydroxylation of a specific proline found in each of NODD/CODD domains of HIF1A and also hydroxylates HIF2A with preference for CODD site of HIF1A/HIF1B. After hydroxylation, HIFs undergo proteasomal degradation via VHL (von Hippel-Lindau) ubiquitition complex. However, under hypoxic conditions, the hydroxylation reaction is tempered which allows HIFs to escape degradation process followed by their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxia-inducible genes. HIF1-PH, through regulating HIF1 stability, is involved in various hypoxia-influenced processes such as angiogenesis in retil/cardiac functiolity as well as tumor angiogenesis, and defecticve EGLN1 have been linked to ECYT3 (familial erythrocytosis type 3).

General readings

Appelhoff,R.J., et al., (2004) J. Biol. Chem. 279 (37), 38458-38465

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EGLN1 / PHD2 antibody

  Rabbit Polyclonal EGLN1/PHD2 Antibody  
Western Blot: PHD2/HIF Prolyl Hydroxylase 2 Antibody [TA336261] -   Western Blot analysis of human PHD2, using TA336261. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10ng/lane) and whole cell lysate from HeLa cells. Rabbit Purified Hu, Ms C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

  Rabbit Polyclonal Antibody against HIF Prolyl Hydroxylase 2  
Western blot analysis of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 and PHD3 (10 ng/lane), HeLa whole cell lysate and MEFs.. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

  Mouse Monoclonal HIF Prolyl Hydroxylase 2 Antibody (366G/76/3)  
Western Blot: HIF Prolyl Hydroxylase 2 Antibody (366G/76/3) - Western blot analysis in HeLa whole cell extracts using anti-EGLN1. Mouse IgG1 366G/76/3 Purified Hu C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

  Anti-EGLN1 Rabbit Polyclonal Antibody  
Predicted band size: 46 kDa . Positive control: OVCAR8, COS7, U2OS, SH-SY5Y and Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: OVCAR8 cell lysate Lane2: COS7 cell lysate Lane3: U2OS cell lysate Lane4: SH-SY5Y cell lysate Lane5: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

  EGLN1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from HepG2 cells, treated with Anisomycin (0.5uM, 5hours), using IKK-? (Phospho-Ser85) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

  Rabbit Polyclonal EGLN1/PHD2 Antibody  
Western Blot: PHD2/HIF Prolyl Hydroxylase 2 Antibody [TA336284] - Detection of PHD2 in mouse kidney lysate using NB 100-2219. ECL exposure, 20 seconds. Rabbit Purified Hu, Ms C, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

  Rabbit Polyclonal Anti-EGLN1 Antibody  
WB Suggested Anti-EGLN1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

Immunofluorescence: HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue). Mouse IgG2b AT1E9 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

EGLN1 / PHD2 antibody

Immunofluorescence: HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue). Mouse IgG2b AT1E9 Purified Hu E, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

EGLN1 / PHD2 (1-50) antibody

  Western blot analysis in HeLa whole cell extracts using HIF Prolyl Hydroxylase 2 Antibody. Mouse IgG1 366G/76/3 Aff - Purified Bov, Hu, Rt P, WB
0.1 ml / €340.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

EGLN1 - human gene knockout kit via CRISPR

EGLN1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EGLN1 - human gene knockout kit via CRISPR

EGLN1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EGLN1 - human gene knockout kit via CRISPR

EGLN1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EGLN1 - human gene knockout kit via CRISPR

EGLN1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Egln1 - mouse gene knockout kit via CRISPR

Egln1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Egln1 - mouse gene knockout kit via CRISPR

Egln1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Egln1 - mouse gene knockout kit via CRISPR

Egln1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Egln1 - mouse gene knockout kit via CRISPR

Egln1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EGLN1 / PHD2

  Recombinant protein of human egl nine homolog 1 (C. elegans) (EGLN1)  
EGLN1 / PHD2 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, EGLN1 (mGFP-tagged) - Human egl nine homolog 1 (C. elegans) (EGLN1), 200 uL, >10^7 TU/mL

Lenti ORF particles, EGLN1 (mGFP-tagged) - Human egl nine homolog 1 (C. elegans) (EGLN1), 200 uL, >10^7 TU/mL  
200 µl / €1,020.00
  OriGene Technologies, Inc.

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