EGLN1 / PHD2 antibody

Principal name

EGLN1 / PHD2 antibody

Alternative names for EGLN1 / PHD2 antibody

EGLN-1, C1orf12, HIF-PH2, HIF-prolyl hydroxylase 2, HPH-2, SM-20, Egl nine homolog 1, Hypoxia-inducible factor prolyl hydroxylase 2, Prolyl hydroxylase domain-containing protein 2

SwissProt ID

P59722 (Rat), Q91YE3 (Mouse), Q9GZT9 (Human)

Gene ID

54583 (EGLN1), 112405 (Egln1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Ze (Zebrafish), African clawed frog, Mky (Monkey), Bov (Bovine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Flow Cytometry (F), Neutralisation (NEUT), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Frozen Sections (C)

Background of EGLN1 / PHD2 antibody

HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) that mediate important physiological responses to hypoxia by modulating HIF1alpha levels. The HIF-alpha is regulated by hydroxylation, both by a family of PHDs leading to ubiquitition and proteasomal degradation, and by transcriptiol ictivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). Under normoxic conditions, HIF Prolyl Hydroxylase 2 (HIF1-PH; also called PHD1 or Egln2) catalyzes the post-translatiol formation of 4-hydroxyproline through hydroxylation of a specific proline found in each of NODD/CODD domains of HIF1A and also hydroxylates HIF2A with preference for CODD site of HIF1A/HIF1B. After hydroxylation, HIFs undergo proteasomal degradation via VHL (von Hippel-Lindau) ubiquitition complex. However, under hypoxic conditions, the hydroxylation reaction is tempered which allows HIFs to escape degradation process followed by their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxia-inducible genes. HIF1-PH, through regulating HIF1 stability, is involved in various hypoxia-influenced processes such as angiogenesis in retil/cardiac functiolity as well as tumor angiogenesis, and defecticve EGLN1 have been linked to ECYT3 (familial erythrocytosis type 3).

General readings

1. McMahon, Stephanie, et al. Molecular Basis of Cell and Developmental Biology: Transforming Growth Factor B1 INduces Hypoxia-inducible Factor-1 Stabilization through Selective Inhibition of PHD@ Expression. J.Biol. Chem., 281: 24171-24181, 2006
2. Grover, T. R., Asikainen, T. M., Kinsella, J. P., Abman, S. H., and White, C. W. (2007) Hypoxia inducible factors HIF- 1alpha and HIF-2alpha are decreased in an experimental model of severe respiratory distress syndrome in preterm lambs, 00372.02006.
3. Kondo, S., Seo, S. Y., Yoshizaki, T., Wakisaka, N., Furukawa, M., Joab, I., Jang, K. L., and Pagano, J. S. (2006) EBV Latent Membrane Protein 1 Up-regulates Hypoxia-Inducible Factor 1{alpha} through Siah1-Mediated Down-regulation of Prolyl Hydroxylases 1 and 3 in Nasopharyngeal Epithelial Cells, 66, 9870-9877.
4. Stiehl, D. P., Wirthner, R., Koditz, J., Spielmann, P., Camenisch, G., and Wenger, R. H. (2006) Increased Prolyl 4-Hydroxylase Domain Proteins Compensate for Decreased Oxygen Levels: EVIDENCE FOR AN AUTOREGULATORY OXYGEN-SENSING SYSTEM, 281, 23482-23491.
5. Lu H, Dalgard C, Mohyeldin A, McFate T, Tait A.S., Verma A, The Journal of Biological Chemestry, vol. 280, 51, pg 41928-41939 (2005)
5. Stiehl DP, Wirthner R, Koditz J, Spielmann P, Camenisch G, Wenger RH. Institute of Physiology and Center for Integrative Human Physiology, University of Zurich, CH-8057 Zurich, Switzerland. daniel.stiehl@access.unizh.ch
7. Lu H, Dalgard CL, Mohyeldin A, McFate T, Tait AS, Verma A. Department of Neurology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814, USA.
8. Baek, J.H. et al. Spermidine/Spermine N1-Acetyltransferase-1 Binds to Hypoxia-inducible Factor-1alpha (HIF-1alpha) and RACK1 and Promotes Ubiquitination and Degradation of HIF-1alpha. J. Biol. Chem. 10.1074/jbcM705627200, Sep 2007
9. Baek, J.H. et al Spermidine/Spermine-N1-Acetyltransferase 2 Is an Essential Component of the Ubiquitin Ligase Complex That Regulates Hypoxia-inducible Factor 1 alpha. J. Biol. Chem. 10.1074/jbcM703504200, May 2007
3. Bex, C., et al. A yeast two-hybrid system reconstituting substrate recognition of the von Hippel-Lindau tumor suppressor protein. Nucleic Acids Res. published 5 November 2007, 10.1093/nar/gkm932.
4. Choi., Y., et al. ADAP12 Regulates Human Blood-Retinal Barrier Formation by Downregulation of Hypoxia-Inducible Factor-1alpha. J.Neurosci., Apr 2007; 27: 4472 - 4481
5. Colla, S., et al.The new tumor suppressor gene inhibitor of growth family member 4 (ING4) regulates the production of pro-angiogenic molecules by myeloma cells and suppresses hypoxia inducible factor (HIF)-1alpha activity being involved in myeloma-induced angiogenesis. Blood, Sep 2007;
doi:10.1182/blood-2007-02-074617.
6. Chang, E., et al. Age Decreases Endothelial Progenitor Cell Recruitment Through Decreases in Hypoxia-Inducible Factor 1alpha Stabilization During Ischemia. Circulation, Nov 2007
7. Bex, C., et al. A yeast two-hybrid system reconstituting substrate recognition of the von Hippel-Lindau tumor suppressor protein. Nucleic Acids Res. 35(21): e142, 2007.
8. Chang EI, Loh SA, Ceradini DJ, et al. Age Decreases Endothelial Progenitor Cell Recruitment Through Decreases in Hypoxia-Inducible Factor 1{alpha} Stabilization During Ischemia. Circulation. 2007 December 11, 2007;116(24):2818-29.
9. Mikhaylova, O., M. L. Ignacak, et al. (2008). The von Hippel-Lindau tumor suppressor protein and Egl 9-type proline hydroxylases regulate the large subunit of RNA Polymerase II in response to oxidative stress. Mol. Cell. Biol.: MCB.01231-07.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EGLN1 / PHD2 antibody

Western Blot: PHD2/HIF Prolyl Hydroxylase 2 Antibody [TA336261] -   Western Blot analysis of human PHD2, using TA336261. Samples: Recombinant FLAG-His-PHD1, PHD2 or PHD3 (10ng/lane) and whole cell lysate from HeLa cells. Rabbit Purified Hu, Ms C, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 (Sample Pack) antibody

Immunohistochemistry:  HIF Prolyl Hydroxylases Antibody [NB100-903PHD] - Staining of lung, vascular endothelium. Rabbit Aff - Purified Hu, Ms, Rt F, ICC/IF, IP, P, WB
7 vials / €700.00
  Novus Biologicals Inc.

EGLN1 / PHD2 antibody

Western blot analysis of human PHD2. Samples: Recombinant FLAG-His-PHD1, PHD2 and PHD3 (10 ng/lane), HeLa whole cell lysate and MEFs.. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

Western Blot: HIF Prolyl Hydroxylase 2 Antibody (366G/76/3) - Western blot analysis in HeLa whole cell extracts using anti-EGLN1. Mouse IgG1 366G/76/3 Purified Hu C, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

Predicted band size: 46 kDa . Positive control: OVCAR8, COS7, U2OS, SH-SY5Y and Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: OVCAR8 cell lysate Lane2: COS7 cell lysate Lane3: U2OS cell lysate Lane4: SH-SY5Y cell lysate Lane5: Hela cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

Western blot analysis of extracts of various cell lines, using EGLN1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

Western Blot: PHD2/HIF Prolyl Hydroxylase 2 Antibody [TA336284] - Detection of PHD2 in mouse kidney lysate using NB 100-2219. ECL exposure, 20 seconds. Rabbit Purified Hu, Ms C, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

WB Suggested Anti-EGLN1 Antibody Titration: 1.25 ug/ml; ELISA Titer: 1:12500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGLN1 / PHD2 antibody

Immunofluorescence: HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue). Mouse IgG2b AT1E9 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

EGLN1 / PHD2 antibody

Immunofluorescence: HeLa cells were stained with monoclonal anti-EGLN1 antibody (Green). Nucleus and Actin were stained by Phalloidin-TRITC (Red) or Hoechst 33342 (Blue). Mouse IgG2b AT1E9 Purified Hu E, ICC/IF, WB
50 µl / €230.00
  Acris Antibodies GmbH

EGLN1 / PHD2 antibody

EGLN1 / PHD2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

EGLN1 / PHD2 antibody

Staining of Egln1 on renal tubular epithelium in mouse kidney cortex using Egln1 polyclonal antibody ( Cat # PAB12122 ) at 2.5µg/ml. Rabbit Ms C, IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

EGLN1 / PHD2 antibody

Western blot analysis of EGLN1 using EGLN1 polyclonal antibody ( Cat # PAB12440 ). Samples : Recombinant FLAG-His-EGLN2, EGLN1 and EGLN3 ( 10 ng/lane ), HeLa whole cell lysate and MEF. Rabbit Hu C, IP, WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

EGLN1 / PHD2 antibody

Human HepG2; WB Suggested Anti-EGLN1 Antibody Titration: 1.25ug/ml. ELISA Titer: 1:12500. Positive Control: HepG2 cell lysate; EGLN1 antibody - C-terminal region (ARP34608_T100) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Can, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

EGLN1 / PHD2 (1-50) antibody

  Western blot analysis in HeLa whole cell extracts using HIF Prolyl Hydroxylase 2 Antibody. Mouse IgG1 366G/76/3 Aff - Purified Bov, Hu, Rt P, WB
0.1 ml / €340.00
  Neuromics Antibodies

EGLN1 / PHD2 antibody

EGLN1 / PHD2 Rabbit Aff - Purified Hu, Rt F, WB
0.1 ml / €360.00
  Novus Biologicals Inc.

EGLN1 / PHD2 antibody

EGLN1 / PHD2 Rabbit Aff - Purified Hu, Rt F, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

EGLN1 / PHD2 antibody

EGLN1 / PHD2 Rabbit Aff - Purified Hu, Rt F, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

EGLN1 / PHD2 antibody

EGLN1 / PHD2 Rabbit Aff - Purified Hu, Rt F, WB
0.1 ml / €390.00
  Novus Biologicals Inc.

EGLN1 / PHD2 control peptide

EGLN1 / PHD2 Purified NEUT
50 µg / €340.00
  Novus Biologicals Inc.

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