EGLN2 / PHD1 antibody

Principal name

EGLN2 / PHD1 antibody

Alternative names for EGLN2 / PHD1 antibody

HIF-PH1, HIF PH1, HPH-1, HPH-3, EIT6, Egl nine homolog 2, Hypoxia-inducible factor prolyl hydroxylase 1, HIF-prolyl hydroxylase 1, Prolyl hydroxylase domain-containing protein 1, Estrogen-induced tag 6

SwissProt ID

Q91YE2 (Mouse), Q96KS0 (Human)

Gene ID

112398 (EGLN2), 112406 (Egln2)

Available reactivities

Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), GP (Guinea Pig), Por (Porcine), Eq (Equine), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of EGLN2 / PHD1 antibody

HIF prolyl 4-hydroxylases (PHD) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) that facilitate physiological reactions to hypoxic stress by modulating HIF1alpha expression levels. HIF-alpha is regulated by hydroxylation, both by a family of PHDs leading to ubiquitition and proteasomal degradation, and by transcriptiol ictivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). HIF Prolyl Hydroxylase 1 (HIF-PH1/EGLN2/PHD1) is localized in the nucleus and interacts with SIAH2, LIMD1, WTIP and AJUBA. In normal oxygen conditions, HIF-PH1 catalyzes the post-translatiol formation of 4-hydroxyproline in HIF- alpha and hydroxylates a specific prolines found in NODD/CODD of HIF1A, and also hydroxylates HIF2A with preference for CODD site for HIF1A/HIF2A. VHL (von Hippel-Lindau ubiquitition) complex then targets hydroxylated HIFs to facilitate their proteasomal degradation. Under hypoxic stress conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. HIF-PH1 is involved in regulating hypoxia tolerance and apoptosis in cardiac and skeletal muscle, tumor angiogenesis and susceptibility to normoxic oxidative neurol death.

General readings

Appelhoff,R.J., et al., (2004) J. Biol. Chem. 279 (37), 38458-38465

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EGLN2 / PHD1 antibody

  EGLN2 mouse monoclonal antibody, clone OTI7H3 (formerly 7H3)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-EGLN2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA808584)(1:150) Mouse IgG1 OTI7H3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

EGLN2 / PHD1 antibody

  Rabbit Polyclonal Anti-EGLN2 Antibody  
WB Suggested Anti-EGLN2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human Placenta Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

EGLN2 / PHD1 antibody

  Rabbit Polyclonal EGLN2/PHD1 Antibody  
Western Blot: HIF Prolyl Hydroxylase 1 Antibody [TA336304] - Recombinant epitope-tagged PHD1, PHD2 and PHD3 (10 ng/lane) or whole cell lysate from HeLa (human) or MEF (mouse) cells.  Antibody: Affinity purified rabbit anti-PHD1 (Cat. No. TA336304) used at Rabbit Purified Hu, Ms, Rt C, ICC/IF, IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN2 / PHD1 antibody

  Rabbit Polyclonal Anti-EGLN2 Antibody  
WB Suggested Anti-EGLN2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human heart Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EGLN2 / PHD1 antibody

  Rabbit Polyclonal Anti-EGLN2 Antibody  
EGLN2 / PHD1 Rabbit IgG Purified Can, Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

EGLN2 / PHD1 antibody

  EGLN2 mouse monoclonal antibody, clone OTI5E11 (formerly 5E11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGLN2 (RC219191, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EGLN2.(1:2000) Mouse IgG1 OTI5E11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

EGLN2 / PHD1 antibody

Western blot analysis of EGLN2 Antibody (Center) (AP18002PU-N) in MDA-MB231 cell line lysates (35ug/lane). EGLN2 (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EGLN2 / PHD1 (N-term) antibody

EGLN2 Antibody (N-term) (Cat. #AP51387PU-N) western blot analysis in MDA-MB231 cell line lysates (35µg/lane).This demonstrates the EGLN2 antibody detected the EGLN2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EGLN2 / PHD1 antibody

EGLN2 / PHD1 Mouse IgG2a 2G3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EGLN2 / PHD1 antibody

EGLN2 / PHD1 Rabbit Hu, Ms WB
0.1 ml / €470.00
  Abnova Taiwan Corp.

EGLN2 / PHD1 antibody

Human Placenta; WB Suggested Anti-EGLN2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Human Placenta; EGLN2 antibody - N-terminal region (ARP34483_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified Can, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

EGLN2 / PHD1 antibody

Human Muscle; WB Suggested Anti-EGLN2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Human Muscle; EGLN2 antibody - middle region (ARP39775_P050) in Human Muscle cells using Western Blot Rabbit Aff - Purified Can, Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EGLN2 / PHD1 (transcript variant 1)

  Recombinant protein of human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 1  
EGLN2 / PHD1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EGLN2 / PHD1 (N-term HIS tag, transcript variant 3)

  Purified recombinant protein of Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3, with N-terminal HIS tag, expressed in E.Coli, 50ug  
EGLN2 / PHD1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

EGLN2 / PHD1

EGLN2 / PHD1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

EGLN2 / PHD1

EGLN2 / PHD1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, EGLN2 (mGFP-tagged) - Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, EGLN2 (mGFP-tagged) - Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, EGLN2 (mGFP-tagged) - Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, EGLN2 (mGFP-tagged) - Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, EGLN2 (Myc-DDK tagged) - Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, EGLN2 (Myc-DDK tagged) - Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, EGLN2 (Myc-DDK tagged) - Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3 , 200 uL, >10^7 TU/mL

Lenti ORF particles, EGLN2 (Myc-DDK tagged) - Human egl nine homolog 2 (C. elegans) (EGLN2), transcript variant 3 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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