EGLN3 / PHD3 antibody

Principal name

EGLN3 / PHD3 antibody

Alternative names for EGLN3 / PHD3 antibody

HPH-1,HPH-3, HIF-PH3, HIF PH3, Egl nine homolog 3, Hypoxia-inducible factor prolyl hydroxylase 3, HIF-prolyl hydroxylase 3, Prolyl hydroxylase domain-containing protein 3

SwissProt ID

Q62630 (Rat), Q91UZ4 (Mouse), Q9H6Z9 (Human)

Gene ID

112399 (EGLN3)

Available reactivities

Hu (Human), Rt (Rat), Ms (Mouse), Bov (Bovine), Chk (Chicken), African clawed frog, Can (Canine), Mky (Monkey), Xen (Xenopus), Prim (Primate), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of EGLN3 / PHD3 antibody

HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) which are known for their role in mediating physiological responses to hypoxic stress via modulation of HIF1alpha expression levels. HIF-alpha subunit is regulated by hydroxylation, both by a family of PHDs leading to ubiquitition and proteasomal degradation, and by transcriptiol ictivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). When oxygen levels are normal, HIF Prolyl Hydroxylase 3 (HIF-PH3/ PHD3/ Egln3) hydroxylates a specific proline found in HIF1A'a NODD/CODD domains and also hydroxylates HIF2A with preference for CODD site for HIF1A/HIF2A. Once hydroxylated, HIFs undergo proteasomal degradation via von Hippel-Lindau (VHL) ubiquitition complex. Upon hypoxic trigger, the hydroxylation reaction is tempered which let HIFs to escape degradation process leading to their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. HIF-PH3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Besides HIFs, in hypoxic conditions HIF-PH3 also hydroxylates PKM (thereby limiting glycolysis) and hydroxylates/regulates the stability of ADRB2. In cardiomyocytes, HIF-PH3 inhibits Bcl2's anti-apoptotic effect by disrupting the BAX-BCL2 complex, and in neurons, HIF-PH3 has a NGF-induced proapoptotic effect mediated via CASP3 activity regulation. HIF-PH3 also plays a crucial role in D damage response by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway.

General readings

Epstein A.C.R., et al. (2001) Cell. 107:43-54Lee S., et al. (2000) Cancer Cell. 8:155-167

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EGLN3 / PHD3 antibody

TA302471 (0.1µg/ml) staining of Human Muscle lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

Western Blot: HIF Prolyl Hydroxylase 3 Antibody (EG188e/d5) - Western blot analysis of HIF Prolyl Hydroxylase 3 in Lane 1: Cobalt chloride treated COS-7 nuclear extracts and Lane 2: Untreated COS-7 nuclear extracts. Mouse IgG1 EG188e/d5 Purified Bov, Hu, Ms, Prim, Rt C, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

Western Blot: PHD3 Antibody (A1.10) - Western Blot on PHD3 overexpression lysates (+) and empty vector lysates (-). Mouse IgG2a A1.10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

Western Blot: HIF Prolyl Hydroxylase 3 Antibody - Detection of Human and Rat PHD3 by Western Blot. Samples: (A) Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. (B) Lysate from rat cells before or 9 hours after inducing hypoxia. Antibody: Affinity purified rabbit anti-PHD3. Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using EGLN3 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

Western Blot: PHD3/HIF Prolyl Hydroxylase 3 Antibody [TA336303] -  Western Blot analysis of human PHD3, using TA336303. Samples:  MEF whole cell lysate. Rabbit Purified Hu, Ms, Prim IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

WB Suggested Anti-EGLN3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

Immunofluorescence of purified MaxPab antibody to EGLN3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

EGLN3 / PHD3 antibody

Western Blot analysis of EGLN3 expression in transfected 293T cell line (H00112399-T03) by EGLN3 MaxPab polyclonal antibody.

Lane 1: EGLN3 transfected lysate(27.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

EGLN3 / PHD3 antibody

EGLN3 / PHD3 Mouse IgG2a 3C5 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

EGLN3 / PHD3 antibody

EGLN3 polyclonal antibody ( Cat # PAB7480 ) ( 0.1 µg/ml ) staining of human muscle lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

EGLN3 / PHD3 antibody

Human Brain; WB Suggested Anti-EGLN3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human brain; EGLN3 antibody - C-terminal region (ARP52402_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

EGLN3 / PHD3 antibody

EGLN3 / PHD3 Rabbit Aff - Purified Hu, Rt WB
0.1 ml / €360.00
  Novus Biologicals Inc.

EGLN3 / PHD3 antibody

EGLN3 / PHD3 Rabbit Aff - Purified Hu, Rt WB
0.1 ml / €390.00
  Novus Biologicals Inc.

EGLN3 / PHD3 antibody

EGLN3 / PHD3 Rabbit Aff - Purified Hu, Rt WB
0.1 ml / €390.00
  Novus Biologicals Inc.

EGLN3 / PHD3 antibody

EGLN3 / PHD3 Rabbit Aff - Purified Hu, Rt WB
0.1 ml / €390.00
  Novus Biologicals Inc.

EGLN3 / PHD3 antibody

EGLN3 / PHD3 Rabbit Aff - Purified Hu, Rt WB
0.1 ml / €390.00
  Novus Biologicals Inc.

EGLN3 / PHD3 antibody

EGLN3 / PHD3 Rabbit Aff - Purified Hu, Rt WB
25 µl / €200.00
  Novus Biologicals Inc.

EGLN3 / PHD3 antibody

EGLN3 / PHD3 Rabbit Biotin Hu, Ms WB
0.1 ml / €360.00
  Novus Biologicals Inc.

EGLN3 / PHD3 antibody

EGLN3 / PHD3 Rabbit DyLight488 Hu, Ms WB
0.1 ml / €390.00
  Novus Biologicals Inc.

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