EGLN3 / PHD3 antibody

Principal name

EGLN3 / PHD3 antibody

Alternative names for EGLN3 / PHD3 antibody

HPH-1,HPH-3, HIF-PH3, HIF PH3, Egl nine homolog 3, Hypoxia-inducible factor prolyl hydroxylase 3, HIF-prolyl hydroxylase 3, Prolyl hydroxylase domain-containing protein 3

SwissProt ID

Q62630 (Rat), Q91UZ4 (Mouse), Q9H6Z9 (Human)

Gene ID

112399 (EGLN3), 112407 (Egln3), 54702

Available reactivities

Hu (Human), Bov (Bovine), Chk (Chicken), Ms (Mouse), Rt (Rat), African clawed frog, Can (Canine), Prim (Primate), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Sh (Sheep), Ze (Zebrafish)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Paraffin Sections (P), Immunoprecipitation (IP)

Background of EGLN3 / PHD3 antibody

HIF prolyl 4-hydroxylases (PHDs) are proyl hydroxylase domain-containing enzymes (PHD1/ Egln2, PHD2/ Egln1, PHD3/ Egln3, and P4H-TM) which are known for their role in mediating physiological responses to hypoxic stress via modulation of HIF1alpha expression levels. HIF-alpha subunit is regulated by hydroxylation, both by a family of PHDs leading to ubiquitition and proteasomal degradation, and by transcriptiol ictivation following asparaginyl hydroxylation by FIH (factor inhibiting HIF). When oxygen levels are normal, HIF Prolyl Hydroxylase 3 (HIF-PH3/ PHD3/ Egln3) hydroxylates a specific proline found in HIF1A'a NODD/CODD domains and also hydroxylates HIF2A with preference for CODD site for HIF1A/HIF2A. Once hydroxylated, HIFs undergo proteasomal degradation via von Hippel-Lindau (VHL) ubiquitition complex. Upon hypoxic trigger, the hydroxylation reaction is tempered which let HIFs to escape degradation process leading to their nuclear translocation, heterodimerization with HIF1B, and increased expression of hypoxy-inducible genes. HIF-PH3 is the most important isozyme in limiting physiological activation of HIFs (particularly HIF2A) in hypoxia. Besides HIFs, in hypoxic conditions HIF-PH3 also hydroxylates PKM (thereby limiting glycolysis) and hydroxylates/regulates the stability of ADRB2. In cardiomyocytes, HIF-PH3 inhibits Bcl2's anti-apoptotic effect by disrupting the BAX-BCL2 complex, and in neurons, HIF-PH3 has a NGF-induced proapoptotic effect mediated via CASP3 activity regulation. HIF-PH3 also plays a crucial role in D damage response by hydroxylating TELO2, promoting its interaction with ATR which is required for activation of the ATR/CHK1/p53 pathway.

General readings

Epstein A.C.R., et al. (2001) Cell. 107:43-54Lee S., et al. (2000) Cancer Cell. 8:155-167

16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EGLN3 / PHD3 antibody

  Goat Polyclonal Antibody against EGLN3  
TA302471 (0.1µg/ml) staining of Human Muscle lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

  Mouse Monoclonal HIF Prolyl Hydroxylase 3 Antibody (EG188e/d5)  
Western Blot: HIF Prolyl Hydroxylase 3 Antibody (EG188e/d5) - Western blot analysis of HIF Prolyl Hydroxylase 3 in Lane 1: Cobalt chloride treated COS-7 nuclear extracts and Lane 2: Untreated COS-7 nuclear extracts. Mouse IgG1 EG188e/d5 Purified Bov, Hu, Ms, Prim, Rt C, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

  Mouse Monoclonal HIF Prolyl Hydroxylase 3 Antibody (A1.10)  
Western Blot: PHD3 Antibody (A1.10) - Western Blot on PHD3 overexpression lysates (+) and empty vector lysates (-). Mouse IgG2a A1.10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

  Rabbit Polyclonal HIF Prolyl Hydroxylase 3 Antibody  
Western Blot: HIF Prolyl Hydroxylase 3 Antibody - Detection of Human and Rat PHD3 by Western Blot. Samples: (A) Lysate from cells that were transiently transfected with PHD1, PHD2, PHD3 or PHD4 constructs or in vitro translated PHD1, PHD2, PHD3, PHD4. (B) Lysate from rat cells before or 9 hours after inducing hypoxia. Antibody: Affinity purified rabbit anti-PHD3. Rabbit IgG Purified Hu, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

  Anti-EGLN3 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human liver cancer tissue using EGLN3 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

  Rabbit Polyclonal EGLN3/PHD3 Antibody  
Western Blot: PHD3/HIF Prolyl Hydroxylase 3 Antibody [TA336303] -  Western Blot analysis of human PHD3, using TA336303. Samples:  MEF whole cell lysate. Rabbit Purified Hu, Ms, Prim IP, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EGLN3 / PHD3 antibody

  Rabbit Polyclonal Anti-EGLN3 Antibody  
WB Suggested Anti-EGLN3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Sh, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EGLN3 / PHD3

  Recombinant protein of human egl nine homolog 3 (C. elegans) (EGLN3)  
EGLN3 / PHD3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

EGLN3 / PHD3 (1-239, His-tag)

EGLN3 / PHD3 Human Purified 0.25 mg/ml (determined by Bradford assay) E. coli
0.1 mg / €730.00
  Acris Antibodies GmbH

EGLN3 / PHD3 (1-239, His-tag)

EGLN3 / PHD3 Human Purified 0.25 mg/ml (determined by Bradford assay) E. coli
20 µg / €300.00
  Acris Antibodies GmbH

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, EGLN3 (mGFP-tagged) - Human egl nine homolog 3 (C. elegans) (EGLN3), 200 uL, >10^7 TU/mL

Lenti ORF particles, EGLN3 (mGFP-tagged) - Human egl nine homolog 3 (C. elegans) (EGLN3), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, EGLN3 (Myc-DDK tagged) - Human egl nine homolog 3 (C. elegans) (EGLN3) , 200 uL, >10^7 TU/mL

Lenti ORF particles, EGLN3 (Myc-DDK tagged) - Human egl nine homolog 3 (C. elegans) (EGLN3) , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Egln3 (GFP-tagged) - Mouse EGL nine homolog 3 (C. elegans) (Egln3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Egln3 (GFP-tagged) - Mouse EGL nine homolog 3 (C. elegans) (Egln3), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Egln3 (Myc-DDK-tagged) - Mouse EGL nine homolog 3 (C. elegans) (Egln3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Egln3 (Myc-DDK-tagged) - Mouse EGL nine homolog 3 (C. elegans) (Egln3), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Egln3 (GFP-tagged ORF) - Rat EGL nine homolog 3 (C. elegans) (Egln3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Egln3 (GFP-tagged ORF) - Rat EGL nine homolog 3 (C. elegans) (Egln3), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Egln3 (Myc-DDK-tagged ORF) - Rat EGL nine homolog 3 (C. elegans) (Egln3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Egln3 (Myc-DDK-tagged ORF) - Rat EGL nine homolog 3 (C. elegans) (Egln3), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

16 Item(s)

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