EIF2AK2 / PKR antibody

Principal name

EIF2AK2 / PKR antibody

Alternative names for EIF2AK2 / PKR antibody

eIF-2A protein kinase 2, PRKR, Interferon-induced double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, Protein kinase RNA-activated, p68 kinase

SwissProt ID

P19525 (Human), Q03963 (Mouse), Q63184 (Rat), Q9UTE5 (Schpo)

Gene ID

5610 (EIF2AK2), 19106 (Eif2ak2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of EIF2AK2 / PKR antibody

IFN-induced dsRNA-dependent serine/threonine-protein kinase which plays a key role in the innate immune response to viral infection and is also involved in the regulation of signal transduction, apoptosis, cell proliferation and differentiation. Exerts its antiviral activity on a wide range of DNA and RNA viruses including hepatitis C virus (HCV), hepatitis B virus (HBV), measles virus (MV) and herpes simplex virus 1 (HHV-1). Inhibits viral replication via phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (EIF2S1), this phosphorylation impairs the recycling of EIF2S1 between successive rounds of initiation leading to inhibition of translation which eventually results in shutdown of cellular and viral protein synthesis. Also phosphorylates other substrates including p53/TP53, PPP2R5A, DHX9, ILF3, IRS1 and the HHV-1 viral protein US11. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity and phosphorylates CDK1 at 'Tyr-4' upon DNA damage, facilitating its ubiquitination and proteosomal degradation. Either as an adapter protein and/or via its kinase activity, can regulate various signaling pathways (p38 MAP kinase, NF-kappa-B and insulin signaling pathways) and transcription factors (JUN, STAT1, STAT3, IRF1, ATF3) involved in the expression of genes encoding proinflammatory cytokines and IFNs. Activates the NF-kappa-B pathway via interaction with IKBKB and TRAF family of proteins and activates the p38 MAP kinase pathway via interaction with MAP2K6. Can act as both a positive and negative regulator of the insulin signaling pathway (ISP). Negatively regulates ISP by inducing the inhibitory phosphorylation of insulin receptor substrate 1 (IRS1) at 'Ser-312' and positively regulates ISP via phosphorylation of PPP2R5A which activates FOXO1, which in turn up-regulates the expression of insulin receptor substrate 2 (IRS2). Can regulate NLRP3 inflammasome assembly and the activation of NLRP3, NLRP1, AIM2 and NLRC4 inflammasomes.

General readings

Abujiang Pataer, et,al. (2002) Cancer Res ; 62: 2239.
K. D. Ryman, et,al. (2005) J. Virol ; 79: 1487 - 1499.
Susana Guerra, et,al. (2006) J. Biol. Chem ; 281: 18734 - 18745.

  1. Quick-Filters:
  2. Highly Validated (2)

Items 1 to 20 of 70 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF2AK2 / PKR antibody

Immunoperoxidase of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml] Mouse IgG2a 1B9 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EIF2AK2 / PKR antibody

Immunoperoxidase of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml] Mouse IgG1 1D11 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EIF2AK2 / PKR (C-term) antibody

Western blot analysis of PKR in A431 whole cell lysate with PKR antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF2AK2 / PKR (N-term) antibody

Western blot analysis of PKR in Caco-2 whole cell lysate with PKR antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF2AK2 / PKR antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PKR (Ab-451) Antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF2AK2 / PKR antibody

Predicted cell location: Cytoplasm. Positive control: Human breast cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human breast cancer tissue using EIF2AK2 antibody at dilution 1/60, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF2AK2 / PKR antibody

Western blot analysis of PKR expression in Starvation treated K562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF2AK2 / PKR antibody

Western blot analysis of extracts of MCF7 cell line, using Eif2ak2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF2AK2 / PKR pThr446 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue, using PKR pThr446 antibody AP02523PU Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

EIF2AK2 / PKR pThr446 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue, using PKR pThr446 antibody AP02523PU Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

EIF2AK2 / PKR pThr451 antibody

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR antibody (AP02528PU-S; AP02528PU-N). Rabbit Aff - Purified Hu P
0.1 ml / €320.00
  Acris Antibodies GmbH

EIF2AK2 / PKR pThr451 antibody

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR antibody (AP02528PU-S; AP02528PU-N). Rabbit Aff - Purified Hu P
50 µl / €240.00
  Acris Antibodies GmbH

EIF2AK2 / PKR antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKR antibody. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

EIF2AK2 / PKR antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKR antibody. Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

EIF2AK2 / PKR antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR antibody AP02776PU. Rabbit Aff - Purified Hu ICC/IF, P
0.1 ml / €270.00
  Acris Antibodies GmbH

EIF2AK2 / PKR antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKR antibody AP02776PU. Rabbit Aff - Purified Hu ICC/IF, P
50 µl / €230.00
  Acris Antibodies GmbH

EIF2AK2 / PKR antibody

Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

EIF2AK2 / PKR (C-term) antibody

Figure 2. AP07747PU-N EIF2AK2 Antibody Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Human Brain, Cerebellum at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

EIF2AK2 / PKR (N-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EIF2AK2 / PKR (C-term) antibody

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Items 1 to 20 of 70 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  • LinkedIn