EIF2AK3 antibody

Principal name

EIF2AK3 antibody

Alternative names for EIF2AK3 antibody

PEK, PERK, HsPEK, Eukaryotic translation initiation factor 2-alpha kinase 3, PRKR-like endoplasmic reticulum kinase, Pancreatic eIF2-alpha kinase

SwissProt ID

Q9NZJ5 (Human), Q9Z1Z1 (Rat), Q9Z2B5 (Mouse)

Gene ID

9451 (EIF2AK3), 1965 (EIF2S1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Immunoprecipitation (IP), Frozen Sections (C)

Background of EIF2AK3 antibody

The PKR-like endoplasmic reticulum kinase (PERK, also know as Eukaryotic translation initiation factor 2-alpha kinase 3) is a type I transmembrane protein localized to the endoplasmic reticulum (ER). PERK consists of an N-terminal ER luminal domain, a membrane-spanning region, and a cytosolic C-terminal serine/threonine kinase domain (1). The luminal domain of PERK is bound to the ER chaperone GRP78 in unstressed cells (2). PERK activation occurs upon accumulation of misfolded proteins and the ER lumen, which triggers GRP78 dissociation from PERK thereby allowing PERK dimerization and autophosphorylation (3, 4). PERK phosphorylates two established targets: the eukaryotic translation initiation factor 2 alpha (eIF2alpha, (1)) and the Nrf2 transcription factor (5). Phosphorylation of eIF2alpha results in attenuation of translation initiation (6). The translational block also contributes to cell cycle arrest due to loss of the G1 regulatory protein, cyclin D1 (7). PERK-dependent phosphorylation of Nrf2 promotes transcription of phase II detoxifying enzymes which is critically important for elimination of intracellular reactive oxygen species (8). Thus, while inhibiting new protein synthesis and thereby decreasing the ER protein load PERK simultaneously induces expression of genes that help restore cellular redox homeostasis and promote survival.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF2AK3 antibody

Immunohistochemical analysis of PERK staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

EIF2AK3 antibody

Immunohistochemical analysis of PERK staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

EIF2AK3 antibody

Immunohistochemical analysis of PERK staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu ICC/IF, IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

EIF2AK3 antibody

Western blot analysis of extracts from MCF-7 cells, using PEK/PERK (Ab-981) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF2AK3 antibody

Western blot analysis of extracts from HeLa cells and HepG2 cells, using E2AK3 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF2AK3 antibody

Predicted cell location: Cytoplasm. Positive control: Human lung cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using EIF2AK3 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF2AK3 antibody

Predicted band size: 125kDa. Positive control: HT-29 cell lysate. Recommended dilution: 1/200-1000. (Gel: 8%SDS-PAGE Lysate:40 ug Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF2AK3 antibody

293E cells were transfected with PERK-FLAG construct.  Cell lysates were then immunoprecipitated with L5 antibody.  Proteins were then treated with calf intestinal alkaline phosphatase (CIP).  The proteins without CIP treatment were used as the control. Samples were separated by electrophoresis, transferred to nitrocellulose and probed with anti-PERK phospho (Ser713) antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminenescent system. Rabbit IgG Poly6494 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

EIF2AK3 antibody

Western blot analysis of PERK expression in Hela cells Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF2AK3 antibody

Western blot analysis using EIF2AK3 antibody Cat.-No AM06529SU-N against HEK293 (1) and EIF2AK3 (AA: 929-1116)-hIgGFc transfected HEK293 (2) cell lysate. Mouse IgG1 5G5 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

EIF2AK3 antibody

Immunohistochemistry analysis of PERK antibody (Cat.-No.: AP06757PU-N) on paraffin-embedded sections Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EIF2AK3 (601-1115) antibody

Immunohistochemistry staining of Mouse mammary gland samples from lactating mice (L10) using AP09082SU-N PERK antibody. Positive staining signal observed in wild type Mouse sample with anti-PERK staining only (middle image), but not in the knock out mouse sample (Right image) and pre-immune serum staining (Left image). The anti-PERK was diluted 1/1,000 in 5% goat serum in PBS and allowed to incubate for 2h at RT in a humidified chamber. Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA. Rabbit IgG Serum Ms ICC/IF, IP, P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

EIF2AK3 (N-term) antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PERK antibody (N-term Q163), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P
0.4 ml / €370.00
  Acris Antibodies GmbH

EIF2AK3 pThr981 antibody

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using PEK/PERK (Phospho-Thr981) antibody AP55771PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms P
0.1 ml / €340.00
  Acris Antibodies GmbH

EIF2AK3 pThr981 antibody

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using PEK/PERK (Phospho-Thr981) antibody AP55771PU-N (left)or the same antibody preincubated with blocking peptide (right). Rabbit Aff - Purified Hu, Ms P
50 µl / €270.00
  Acris Antibodies GmbH

EIF2AK3 antibody

EIF2AK3 polyclonal antibody (A01), Lot # 060525JCS1 Western Blot analysis of EIF2AK3 expression in PC-12 ( Cat # L012V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

EIF2AK3 antibody

EIF2AK3 Mouse IgG2a 7B10 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EIF2AK3 antibody

Western blot analysis using Eif2ak3 polyclonal antibody ( Cat # PAB10175 ) to detect Eif2ak3 in cell lysates. 300 µg Eif2ak3 over-expressing 293T cell lysate ( lanes 1 & 2 ), or 800 µg wild type ( Lanes 3 & 4 ), and Eif2ak3 knock out ( lanes 5 & 6 ) MEF cell lysate were immunoprecipated with 15 µl anti-Eif2ak3, followed by immunobloting with 1:1000 dilution of Eif2ak3 polyclonal antibody in 5% milk/TBST buffer.
Lane 1, 293T cells over-expressing Myc-Eif2ak3 wt, Lane 2 , 293T cells over-expressing Myc-Eif2ak3 K618A.
Personal Communication. A, Diehl, Univ. of Pennsylvania, Philadelphia, PA. Rabbit Ms E, ICC/IF, IP, P, WB
0.1 ml / €560.00
  Abnova Taiwan Corp.

EIF2AK3 pThr981 antibody

Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma, using PEK/PERK (Phospho-Thr981) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €205.00
  Assay Biotech

EIF2AK3 pThr981 antibody

Immunohistochemistry analysis of paraffin-embedded human prostate carcinoma, using PEK/PERK (Phospho-Thr981) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €310.00
  Assay Biotech

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