EIF3E / EIF3S6 antibody

Principal name

EIF3E / EIF3S6 antibody

Alternative names for EIF3E / EIF3S6 antibody

Eukaryotic translation initiation factor 3 subunit E, eIF-3 p48, INT6, Eukaryotic translation initiation factor 3 subunit 6, Viral integration site protein INT-6 homolog

SwissProt ID

P60228 (Human), P60229 (Mouse), Q5ZLA5 (Chick), Q641X8 (Rat)

Gene ID

3646 (EIF3E), 16341 (Eif3e), 299872

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Mky (Monkey), Rt (Rat), Can (Canine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of EIF3E / EIF3S6 antibody

Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.

General readings

1. Zhou M, Sandercock AM, Fraser CS, Ridlova G, Stephens E, Schenauer MR, Yokoi-Fong T, Barsky D, Leary JA, Hershey JW, Doudna JA, Robinson CV, Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3. Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 105 (47): 18139-44.
PMID: 18599441

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF3E / EIF3S6 (C-term) antibody

  Goat Anti-EIF3E Antibody  
(0.03ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF3E / EIF3S6 antibody

  Rabbit polyclonal Eif3S6 Int6 antibody  
WB using Anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Rabbit IgG Aff - Purified Bov, Can, Hu, Mky, Ms, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

EIF3E / EIF3S6 antibody

  Rabbit Polyclonal Anti-EIF3E Antibody  
EIF3E antibody - N-terminal region validated by WB using B8 mouse cells and HEK293 human cells at 1: 1, 000. Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

EIF3E / EIF3S6 antibody

  Rabbit Polyclonal Anti-EIF3E Antibody  
WB Suggested Anti-EIF3E Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EIF3E / EIF3S6 antibody

Immunohistochemistry (IHC) analyzes of eIF3ε antibody (Cat.-No.: AP01470PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

EIF3E - human gene knockout kit via CRISPR

EIF3E - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EIF3E - human gene knockout kit via CRISPR

EIF3E - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EIF3E - human gene knockout kit via CRISPR

EIF3E - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EIF3E - human gene knockout kit via CRISPR

EIF3E - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Eif3e - mouse gene knockout kit via CRISPR

Eif3e - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Eif3e - mouse gene knockout kit via CRISPR

Eif3e - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Eif3e - mouse gene knockout kit via CRISPR

Eif3e - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Eif3e - mouse gene knockout kit via CRISPR

Eif3e - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EIF3E / EIF3S6

  Recombinant protein of human eukaryotic translation initiation factor 3, subunit E (EIF3E)  
EIF3E / EIF3S6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

EIF3E overexpression lysate

EIF3E overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, EIF3E (mGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit E (EIF3E), 200 uL, >10^7 TU/mL

Lenti ORF particles, EIF3E (mGFP-tagged) - Human eukaryotic translation initiation factor 3, subunit E (EIF3E), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, EIF3E (Myc-DDK tagged) - Human eukaryotic translation initiation factor 3, subunit E (EIF3E) , 200 uL, >10^7 TU/mL

Lenti ORF particles, EIF3E (Myc-DDK tagged) - Human eukaryotic translation initiation factor 3, subunit E (EIF3E) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Eif3e (GFP-tagged) - Mouse eukaryotic translation initiation factor 3, subunit E (Eif3e), 200 uL, >10^7 TU/mL

Lenti ORF particles, Eif3e (GFP-tagged) - Mouse eukaryotic translation initiation factor 3, subunit E (Eif3e), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, Eif3e (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 3, subunit E (Eif3e), 200 uL, >10^7 TU/mL

Lenti ORF particles, Eif3e (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 3, subunit E (Eif3e), 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Eif3e (GFP-tagged ORF) - Rat eukaryotic translation initiation factor 3, subunit E (Eif3e), 200 uL, >10^7 TU/mL

Lenti ORF particles, Eif3e (GFP-tagged ORF) - Rat eukaryotic translation initiation factor 3, subunit E (Eif3e), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

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