EIF3E / EIF3S6 antibody

Principal name

EIF3E / EIF3S6 antibody

Alternative names for EIF3E / EIF3S6 antibody

Eukaryotic translation initiation factor 3 subunit E, eIF-3 p48, INT6, Eukaryotic translation initiation factor 3 subunit 6, Viral integration site protein INT-6 homolog

SwissProt ID

P60228 (Human), P60229 (Mouse), Q5ZLA5 (Chick), Q641X8 (Rat)

Gene ID

3646 (EIF3E), 16341 (Eif3e)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Chk (Chicken), Ze (Zebrafish), African clawed frog, Por (Porcine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of EIF3E / EIF3S6 antibody

Int6 is a candidate tumor suppressor in multiple neoplasms, and in particular, breast and lung cancers. The Int6 locus was initially identified as a common insertion site (CIS) in a genetic screen for transforming sequences in a breast cancer mouse model system. Insertion of mouse mammary tumor virus (MMTV) into this locus results in the production of an amino-terminal truncated gene product. Expression of the truncated Int6 product corresponds to cellular transformation in both in vivo and in vitro systems. This gene product plays a role in regulating translation initiation and is a component of the eIF3 translation initiation complex. There is evidence that suggests that Int6 may impart a negative role in the general translational machinery while promoting an increase in the expression of a subset of stress-responsive genes. Taken together, it is of great interest to further study the mechanism by which Int6 is involved in regulating cell growth.

General readings

1. Zhou M, Sandercock AM, Fraser CS, Ridlova G, Stephens E, Schenauer MR, Yokoi-Fong T, Barsky D, Leary JA, Hershey JW, Doudna JA, Robinson CV, Mass spectrometry reveals modularity and a complete subunit interaction map of the eukaryotic translation factor eIF3. Proceedings of the National Academy of Sciences of the United States of America 2008 Nov 105 (47): 18139-44.
PMID: 18599441

17 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF3E / EIF3S6 (C-term) antibody

(0.03ug/ml) staining of Daudi lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Por E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF3E / EIF3S6 antibody

WB using Anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Rabbit IgG Aff - Purified Bov, Can, Hu, Mky, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF3E / EIF3S6 antibody

Immunohistochemistry (IHC) analyzes of eIF3ε antibody (Cat.-No.: AP01470PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EIF3E / EIF3S6 antibody

WB using Anti-eIF3S6/Int6 antibody shows detection of endogenous eIF3S6/Int6 in whole cell extracts from murine (HC-11 and NIH3T3), monkey (CV-1 and Cos-1), and human (HEK293T) cell lines as well as over-expressed eIF3S6/Int6 (control transfected flag-tagged Int6).The identity of the higher and lower molecular weight bands is unknown. The band at ~48 kDa, indicated by the arrowhead, corresponds to flag-tagged EIF3S6/Int6. Primary antibody was used at 1:1000. Rabbit IgG Aff - Purified Bov, Can, Hu, Mky, Ms, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

EIF3E / EIF3S6 antibody

EIF3S6 polyclonal antibody (A01), Lot # 060111JC01 Western Blot analysis of EIF3S6 expression in Y-79 ( Cat # L042V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

EIF3E / EIF3S6 antibody

Western blot using Eif3e polyclonal antibody ( Cat # PAB10203 ) shows detection of endogenous Eif3e. Specific staining is not present in lysates containing lentiviral knockdown vectors ( shRNA #1 and #2. Control vectors, specifically a scrambled sequence ( Ctl NS ) and a sequence against an unrelated gene ( Ctl #2 ), were also used.
Personal communication, J.Lee, NCI, Bethesda, MD. Rabbit Bov, Hu, Mky, Ms, Rt E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

EIF3E / EIF3S6 antibody

Human Kidney; Kidney; EIF3E antibody - N-terminal region (ARP48175_P050) in Human Kidney cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

EIF3E / EIF3S6 antibody

Hum. Fetal Lung; Host:Rabbit. Target Name:EIF3E. Sample Tissue:Human Fetal Lung. Antibody Dilution: 1.0ug/ml.; EIF3E antibody - middle region (ARP48176_P050) in Hum. Fetal Lung cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

EIF3E / EIF3S6 antibody

Western Blot: eIF3e Antibody [NB110-85528] - Western blot using NB110-85528 shows detection of endogenous eIF3S6/Int6. Specific staining is not present in lysates containing lentiviral knockdown vectors (shRNA #1 and #2. Control vectors, specifically a scrambled sequence (Ctl NS) and a sequence against an unrelated gene (Ctl #2), were also used. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Rt E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

EIF3E / EIF3S6 antibody

EIF3E / EIF3S6 Goat Aff - Purified Bov, Can, Hu, Por E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

EIF3E / EIF3S6 antibody

Immunohistochemistry-Paraffin: eIF3e Antibody [NBP1-84869] - Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in exocrine cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EIF3E / EIF3S6

EIF3E / EIF3S6 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EIF3E / EIF3S6

EIF3E / EIF3S6 Human Purified
2 µg / €300.00
  Abnova Taiwan Corp.

EIF3E / EIF3S6

EIF3E / EIF3S6 Human
10 µg / €380.00
  Abnova Taiwan Corp.

EIF3E / EIF3S6

EIF3E / EIF3S6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

EIF3E / EIF3S6

EIF3E / EIF3S6 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

EIF3E overexpression lysate

EIF3E overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

17 Item(s)

per page
  • LinkedIn