EIF3F / EIF3S5 antibody

Principal name

EIF3F / EIF3S5 antibody

Alternative names for EIF3F / EIF3S5 antibody

eIF-3-epsilon, eIF3 p47, Eukaryotic translation initiation factor 3 subunit F, Eukaryotic translation initiation factor 3 subunit 5

SwissProt ID

O00303 (Human), Q9DCH4 (Mouse)

Gene ID

8665 (EIF3F)

Available reactivities

Hu (Human), Rt (Rat), Bov (Bovine), Chk (Chicken), Dros (Drosophila), Fi (Fish), Mky (Monkey), Ms (Mouse), Chimp (Chimpanzee)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of EIF3F / EIF3S5 antibody

EIF3F is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF 2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

General readings

Gaitanou M., et al (2001). Biochem. J. 355:715-724

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF3F / EIF3S5 antibody

Western blot analysis of extracts from Jurkat cells and COLO205 cells, using EIF3F antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF3F / EIF3S5 (114-125) antibody

Western blot using affinity purified anti-eIF3f antibody shows detection of endogenous eIF3f in lysates from both control HeLa cells (lane 1) and HeLa cells transformed with the kinase cdk11 (lane 2). Cdk11 is responsible for phosphorylating eIF3f in vivo. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:200. This antibody recognizes both phosphorylated and non-phosphorylated eIF3f. Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ. Rabbit IgG Aff - Purified Bov, Chimp, Chk, Dros, Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF3F / EIF3S5 (114-125) antibody

Figure 1. AP07581PU-N EIF3F antibody staining of Formalin-Fixed Paraffin-Embedded Human Brain, cerebellum at 2.5 µg/ml followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and Chromogen. Rabbit Aff - Purified Bov, Chk, Hu, Ms, Rt E, ICC/IF, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

EIF3F / EIF3S5 antibody

EIF3S5 polyclonal antibody (A01), Lot # 070109QCSb Western Blot analysis of EIF3S5 expression in PC-12 ( Cat # L012V1 ). Mouse Serum Hu, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

EIF3F / EIF3S5 antibody

Immunofluorescence of purified MaxPab antibody to EIF3S5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

EIF3F / EIF3S5 antibody

Immunofluorescence of purified MaxPab antibody to EIF3S5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

EIF3F / EIF3S5 antibody

Immunofluorescence of purified MaxPab antibody to EIF3S5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

EIF3F / EIF3S5 antibody

Immunofluorescence of purified MaxPab antibody to EIF3S5 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

EIF3F / EIF3S5 antibody

Western blot using EIF3F polyclonal antibody ( Cat # PAB10008 ) shows detection of endogenous EIF3F in lysates from both control HeLa cells ( lane 1 ) and HeLa cells transformed with the kinase cdk11 ( lane 2 ).
Cdk11 is responsible for phosphorylating EIF3F in vivo.
After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1 : 200.
This antibody recognizes both phosphorylated and non-phosphorylated eIF3f.
Personal Communication, Jiaqi Shi, Univ. Arizona, Tucson, AZ. Bov, Chimp, Chk, Hu, Ms, Rt
0.1 mg / €560.00
  Abnova Taiwan Corp.

EIF3F / EIF3S5 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EIF3F Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
50 µg / €205.00
  Assay Biotech

EIF3F / EIF3S5 antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using EIF3F Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, P, WB
0.1 mg / €310.00
  Assay Biotech

EIF3F / EIF3S5 (N-term) antibody

Human HeLa; WB Suggested Anti-EIF3F Antibody. . Titration: 1.0 ug/ml. . Positive Control: Hela Whole Cell; EIF3F antibody - N-terminal region (ARP61970_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

EIF3F / EIF3S5 (C-term) antibody

Human Fetal heart; WB Suggested Anti-EIF3F Antibody. . Titration: 1.0 ug/ml. . Positive Control: Fetal Heart; EIF3F antibody - C-terminal region (ARP61971_P050) in Human Fetal heart cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

EIF3F / EIF3S5 antibody

Western blot analysis of EIF3F expression in HeLa (A); HepG2 (B); NIH3T3 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

EIF3F / EIF3S5 antibody

Western blot analysis of EIF3F expression in HeLa (A); HepG2 (B); NIH3T3 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

EIF3F / EIF3S5 antibody

Western blot analysis of EIF3F expression in HeLa (A); HepG2 (B); NIH3T3 (C) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt IP, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

EIF3F / EIF3S5 antibody

Western Blot: EIF3F Antibody [NBP1-50037] - Western Blot: EIF3F Antibody [NBP1-50037] - Detection of Human and Mouse eIF3F by Western Blot (h&m) and Immunoprecipitation (h).  Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH3T3 (M; 50 mcg) cells.  Antibodies: Affinity purified rabbit anti-eIF3F antibody used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 6 mcg/mg lysate.  eIF3F was also immunoprecipitated by rabbit anti-eIF3F antibody BL10962, which recognizes an upstream epitope.  For blotting immunoprecipitated eIF3F, the ReliaBLOT®  Reagents and Procedures were used.  Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). Rabbit Aff - Purified Hu, Ms IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

EIF3F / EIF3S5 antibody

EIF3F / EIF3S5 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

EIF3F / EIF3S5 antibody

EIF3F / EIF3S5 Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €290.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EIF3F / EIF3S5

EIF3F / EIF3S5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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