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EIF3K / EIF3S12 antibody

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Principal name

EIF3K / EIF3S12 antibody

Alternative names for EIF3K / EIF3S12 antibody

Eukaryotic translation initiation factor 3 subunit K, eIF-3 p25, eIF-3 p28, PLAC-24, Muscle-specific gene M9 protein, Eukaryotic translation initiation factor 3 subunit 12

SwissProt ID

Q3T0V3 (Bovin), Q9DBZ5 (Mouse), Q9UBQ5 (Human)

Gene ID

27335 (EIF3K)

Ncbi ID

NP_037366.1, 9606, NP_037366, NM_013234

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep)

Available hosts

Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of EIF3K / EIF3S12 antibody

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Belongs to the eIF-3 subunit K family.

General readings

Mayeur G.L., et al. (2003). Eur. J. Biochem, 270: 4133-4139.
Wei Z., et al. (2004). J. Biol. Chem. 279: 34983-34990.

NB100-93304 - EIF3K / EIF3S12
Western Blot and Immunoprecipitation: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303.
GTX101995 - EIF3K / EIF3S12
EIF3K antibody detects EIF3K protein at cytosol on mouse testis by immunohistochemical analysis. Sample: Paraffin-embedded mouse testis. EIF3K antibody (GTX101995) dilution: 1:500.
GTX101995 - EIF3K / EIF3S12
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using EIF3K(GTX101995) antibody at 1:100 dilution.
GTX101995 - EIF3K / EIF3S12
EIF3K antibody detects EIF3K protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EIF3K protein stained by EIF3K antibody (GTX101995) diluted at 1:500.Blue: Hoechst 33343 staining.
GTX101995 - EIF3K / EIF3S12
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE GTX101995 diluted at 1:1000
GTX101996 - EIF3K / EIF3S12
EIF3K antibody [N1C2] detects EIF3K protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: EIF3K protein stained by EIF3K antibody [N1C2] (GTX101996) diluted at 1:500.Blue: Hoechst 33343 staining.
GTX101996 - EIF3K / EIF3S12
Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE GTX101996 diluted at 1:1000
AP22368PU-N - EIF3K / EIF3S12
Immunohistochemical analysis of Paraffin-Embedded Cal27 xenograft using AP22368PU-N EIF3K antibody at 1/100 dilution.
AP22368PU-N - EIF3K / EIF3S12
Western Blot using AP22368PU-N EIF3K antibody at 1/1000 dilution: Sample(30 µg whole cell lysate) A: 293T 12% SDS PAGE.
C15707-1 - EIF3K / EIF3S12
Western blot analysis of extracts from HeLa cells, using EIF3K Antibody. The lane on the right is treated with the synthesized peptide.
AR09912PU-L - EIF3K / EIF3S12
AR09912PU-L - EIF3K / EIF3S12
Recombinant human EIF3K, 1-218aa, His-tagged
AP51398PU-N - EIF3K / EIF3S12
EIF3K Antibody (C-term) (Cat. #AP51398PU-N) western blot analysis in T47D cell line lysates (35µg/lane).This demonstrates the EIF3K antibody detected the EIF3K protein (arrow).
NBP1-91865 - EIF3K / EIF3S12
Immunohistochemistry-Paraffin: eIF3K Antibody [NBP1-91865] - Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
NBP1-91865 - EIF3K / EIF3S12
Immunofluorescence: eIF3K Antibody [NBP1-91865] - Staing of human U-251 MG shows positivity in nucleus but not nucleoli.
NBP1-91865 - EIF3K / EIF3S12
Western Blot: eIF3K Antibody [NBP1-91865] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
CPA4396-100ul - EIF3K / EIF3S12
Western blot analysis of EIF3K expression in SHSY5Y (A); HEK293T (B) whole cell lysates.
TP303529 - EIF3K / EIF3S12
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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF3K / EIF3S12 (C-term) antibody
EIF3K Antibody (C-term) (Cat. #AP51398PU-N) western blot analysis in T47D cell line lysates (35µg/lane).This demonstrates the EIF3K antibody detected the EIF3K protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH
 

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EIF3K / EIF3S12 antibody
Sample (30 ug of whole cell lysate). A: H1299. 12% SDS PAGE. TA308505 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.
+1 additional image

EIF3K / EIF3S12 antibody
Western blot analysis of extracts from HeLa cells, using EIF3K antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF3K / EIF3S12 antibody
Western blot analysis of extracts from HeLa cells, using EIF3K Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €205.00
  Assay Biotech

EIF3K / EIF3S12 antibody
Western blot analysis of extracts from HeLa cells, using EIF3K Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €310.00
  Assay Biotech

EIF3K / EIF3S12 antibody
Western blot analysis of EIF3K expression in SHSY5Y (A); HEK293T (B) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rb, Sh WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

EIF3K / EIF3S12 antibody
Western blot analysis of EIF3K expression in SHSY5Y (A); HEK293T (B) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rb, Sh WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

EIF3K / EIF3S12 antibody
Western blot analysis of EIF3K expression in SHSY5Y (A); HEK293T (B) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rb, Sh WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

EIF3K / EIF3S12 antibody
Western Blot and Immunoprecipitation: eIF3K Antibody [NB100-93304] - Detection of Human eIF3K/EIF3S12 on HeLa whole cell lysate using NB100-93304. eIF3K/EIF3S12 was also immunoprecipitated by rabbit anti-eIF3K/EIF3S12 antibody NB100-93303. Rabbit IgG Aff - Purified Hu IP, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

EIF3K / EIF3S12 antibody
Immunohistochemistry-Paraffin: eIF3K Antibody [NBP1-91865] - Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.
+2 additional images

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EIF3K / EIF3S12
EIF3K / EIF3S12 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.
 

EIF3K / EIF3S12 (1-218, His-tag)
EIF3K / EIF3S12 Human Purified > 95 % E. coli
0.25 mg / €750.00
  Acris Antibodies GmbH
+1 additional image

EIF3K / EIF3S12 (1-218, His-tag)
EIF3K / EIF3S12 Human Purified > 95 % E. coli
50 µg / €300.00
  Acris Antibodies GmbH
+1 additional image

EIF3K / EIF3S12
EIF3K / EIF3S12 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

EIF3K / EIF3S12
EIF3K / EIF3S12 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

EIF3K / EIF3S12
EIF3K / EIF3S12 Human Purified
0.25 mg / €870.00
  Novus Biologicals Inc.

EIF3K / EIF3S12
EIF3K / EIF3S12 Human Purified
50 µg / €380.00
  Novus Biologicals Inc.

Lysates (Overexpressed)

Catalog No.    

EIF3K overexpression lysate
EIF3K overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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  2. Mouse (Ms) (6)
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  5. Monkey (Mky) (3)
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Applications
  1. Western blot / Immunoblot (WB) (10)
  2. Enzyme Immunoassay (E) (3)
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