EIF4B antibody

Principal name

EIF4B antibody

Alternative names for EIF4B antibody

Eukaryotic translation initiation factor 4B, eIF-4B

SwissProt ID

P23588 (Human), Q8BGD9 (Mouse)

Gene ID

1975 (EIF4B), 75705 (Eif4b)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of EIF4B antibody

Eukaryotic initiation factor 4B (eIF4B) is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity (1). Heat shock and serum starvation cause dephosphorylation of eIF4B at multiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insulin treatment correlates well with an observed increase in translation (2-5). Multiple kises, including p70 S6 kise, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 (6). Recently, Ser406 was identified as a novel phosphorylation site regulated by mitogens (7), and the phosphorylation of this site is dependent on MEK and mTOR activity (7). This phosphorylation is shown to be essential for the translatiol activity of eIF4B (7).

General readings

Suzuki,Y., (2004) Genome Res. 14 (9), 1711-1718

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF4B antibody

Immunofluorescence analysis of NIH3T3 cells using EIF4B antibbody Cat.-No AM06769PU-N EIF4B (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Mouse IgG2b 1F5 Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

EIF4B antibody

Predicted band size: 80 kDa . Positive control: 3T3 and HL60 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: 3T3 and HL60 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4B antibody

Western blot analysis of extracts of various cell lines, using EIF4B antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF4B antibody

Host: Rabbit; Target Name: Eif4b; Sample Tissue: Mouse Brain lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

EIF4B antibody

WB Suggested Anti-EIF4B Antibody Titration: 1.25 ug/ml; ELISA Titer: 1: 62500; Positive Control: Jurkat cell lysate; EIF4B is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Rb, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF4B antibody

WB Suggested Anti-EIF4B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: ACHN cell lysate; EIF4B is strongly supported by BioGPS gene expression data to be expressed in Human ACHN cells Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

EIF4B antibody

Western blot analysis of eIF4B expression in HepG2 cells Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF4B antibody

Western blot analysis of eIF4B (Phospho-Ser422) expression in HepG2 cells Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF4B pSer422 antibody

Immunohistochemistry (IHC) analyzes of eIF4B pSer422 antibody (Cat.-No.: AP01821PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

EIF4B pSer422 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B pSer422 antibody (AP09472PU) (Left) and the same antibody preincubated with blocking peptide (Right). Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

EIF4B pSer422 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B pSer422 antibody (AP09472PU) (Left) and the same antibody preincubated with blocking peptide (Right). Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

EIF4B antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B Antibody (#AP09474PU) (Left) or the same antibody preincubated with blocking peptide (Right). Rabbit Aff - Purified Hu P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

EIF4B antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4B Antibody (#AP09474PU) (Left) or the same antibody preincubated with blocking peptide (Right). Rabbit Aff - Purified Hu P, WB
50 µl / €230.00
  Acris Antibodies GmbH

EIF4B antibody

AP11771PU-N EIF4B antibody staining of Formalin-Fixed, Paraffin-Embedded Human breast carcinoma. This antibody was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EIF4B antibody

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4B, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

EIF4B antibody

EIF4B Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

EIF4B antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B polyclonal antibody ( Cat # PAB3774 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

EIF4B antibody

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with EIF4B polyclonal antibody ( Cat # PAB3951 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

EIF4B antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using EIF4B polyclonal antibody ( Cat # PAB12256 ). Rabbit Hu, Ms P
0.1 mg / €390.00
  Abnova Taiwan Corp.

EIF4B pSer422 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using eIF4B (Phospho-Ser422) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
50 µg / €205.00
  Assay Biotech

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