EIF4E antibody

Principal name

EIF4E antibody

Alternative names for EIF4E antibody

eIF-4F 25 kDa subunit, EIF-4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, mRNA cap-binding protein

SwissProt ID

P06730 (Human), P29338 (Rabit), P63073 (Mouse), P63074 (Rat), Q9N0T5 (Bovin)

Gene ID

1977 (EIF4E), 117045 (Eif4e), 13684

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Ze (Zebrafish), Chk (Chicken), Mky (Monkey), Bov (Bovine), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Dot blot (Dot), ELISA (detection) (E(detection)), Frozen Sections (C)

Background of EIF4E antibody

All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). This structure is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G (MIM 600495) (Rychlik et al., 1987 [PubMed 3469651]).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Bendahmane A., et al. (2010) PLoS One. 5(6):e11313.
Pelletier J., et al. (2010) J Gen Virol. 91(Pt 5):1224-8.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF4E antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-EIF4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503042) Mouse IgG1 OTI5D11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

EIF4E antibody

Immunohistochemical analysis of paraffin-embedded stomach cancer (left) and prostate cancer (right) using EIF4E antibody Cat.-No AM06602SU-N with DAB staining. Mouse IgG1 5D11 Ascites Hu E, ICC/IF, P, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

EIF4E antibody

Predicted band size: 25 kDa . Positive control: Hela, HepG2 and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: Hela, HepG2 and 293 cells Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4E antibody

EIF4E Antibody       (Cat. #TA324335) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E       antibody detected the EIF4E       protein (arrow). Mouse IgG1 163CT48.1.9 Purified Hu ICC/IF, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

EIF4E antibody

Western blot analysis of eIF4E expression in FBS treated NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4E antibody

Western blot analysis of extracts of HEK-293 cell lines, using EIF4E antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF4E antibody

MCF-7 cell extract was resolved by electrophoresis, transferred to nitrocellulose,and probed with rabbitanti-eIF4E antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6069 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

EIF4E antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-EIF4E mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502896) Mouse IgG1 OTI6C3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

EIF4E antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (RC207333, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E. Mouse IgG1 OTI6C11 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

EIF4E antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EIF4E (RC207333, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EIF4E. Mouse IgG1 OTI9H1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

EIF4E antibody

Immunohistochemistry (IHC) analyzes of eIF4E antibody (Cat.-No.: AP01512PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EIF4E pSer209 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using elF4E pSer209 antibody AP02491PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

EIF4E pSer209 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using elF4E pSer209 antibody AP02491PU Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

EIF4E antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using elF4E antibody. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

EIF4E antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using elF4E antibody. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

EIF4E (N-term) antibody

AP08407PU-N EIF4E antibody satining of Formalin-Fixed Paraffin-Embedded Human Heart at 1/50 followed by biotinylated Goat anti-Rabbit IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Rabbit IgG Aff - Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

EIF4E Control Peptide

EIF4E
0.25 mg / €250.00
  Acris Antibodies GmbH

EIF4E antibody

EIF4E Rabbit Aff - Purified Chk, Hu, Mky, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

EIF4E (N-term) antibody

Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E antibody (N-term) (AP11780PU-N), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. Rabbit Ig Purified Hu, Ms, Rt, Ze P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

EIF4E pSer209 antibody

Dot blot analysis of anti-EIF4E-pSer209 Phospho-specific Pab (Cat#AP12890PU-N) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml. Rabbit Ig Aff - Purified Hu
0.4 ml / €370.00
  Acris Antibodies GmbH

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