EIF4E3 antibody

Principal name

EIF4E3 antibody

Alternative names for EIF4E3 antibody

Eukaryotic translation initiation factor 4E type 3, eIF-4E type 3, eIF4E type 3, eIF4E-3, eIF-4E3

SwissProt ID

Q8N5X7 (Human), Q9DBB5 (Mouse)

Gene ID

317649 (EIF4E3)

Available reactivities

Bov (Bovine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP)

Background of EIF4E3 antibody

The fly Drosophila melanogaster is one of the most intensively studied organisms in biology and serves as a model system for the investigation of many developmental and cellular processes such as, translation of mRNA which is common to higher eukaryotes, including humans. Translation is divided into three phases: initiation, elongation, and termination (1). Early in the initiation phase, mRNA is recruited to the ribosomes primarily by eukaryotic translation initiation factor 4E (eIF4E) which is a cap binding protein. eIF4E recognizes and binds to the 5?,7-methylguanosine-containing mRNA cap early in initiation phase and this leads to recruitment of mRNA to the ribosomes and subsequent initiation of protein synthesis (2). To date, three different family members of eIF4E have been recognized in mammals, they are: eIF4E-1, eIF4E-2 and eIF4E-3. eIF4E-1 is found in all eukaryotes while eIF4E-2 and eIF4E-3 are found only in metazoans and chordates, respectively (3). The activity of eIF4E proteins is regulated by eIF4E-binding proteins which suppress the activity of eIF4E proteins (4). Once activated, eIF4E-3 has been shown to inhibit the activity of eIF4E-1 protein. It is also known to interact with initiation factor eIF4G (3). eIF4E proteins are implicated in controlling growth of cancer through the P13K/Akt/mTOR signaling pathway, via the phosphorylation of eIF4E-bindnig proteins (5). Once eIF4E-binding proteins are phosphorylated, they can no longer interact with eIF4E proteins and hence are not able to limit the expression of eIF4E proteins in cells. eIF4E proteins are not as abundant in mammalian cells as other initiating factors and any overexpression of these proteins has been shown to transform normal cells into to cancer cells (5). eIF4E-3 is expressed in heart, skeletal muscle, lungs and spleen. It is approximately a 30kDa protein (244 amino acids). The eIF4E3 antibodies were generated using peptide corresponding to N-terminal and C-terminal of Fruit Fly eIF4E3 protein. eIF4E3 antibodies are affinity purified over immobilized antigen based affinity chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF4E3 antibody

WB Suggested Anti-EIF4E3 Antibody Titration: 0.2-1 ug/ml; Positive Control: Human Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EIF4E3 antibody

Western blot analysis of extracts from LOVO cells, using EIF4E3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

EIF4E3 antibody

Western blot analysis of extracts from LOVO cells, using EIF4E3 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

EIF4E3 antibody

Human Liver; WB Suggested Anti-EIF4E3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Liver; EIF4E3 antibody - middle region (ARP41168_P050) in Human Liver cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

EIF4E3 antibody

Western blot analysis of EIF4E3 expression in HEK293T (A); NIH3T3 (B) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

EIF4E3 antibody

Western blot analysis of EIF4E3 expression in HEK293T (A); NIH3T3 (B) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

EIF4E3 antibody

Western blot analysis of EIF4E3 expression in HEK293T (A); NIH3T3 (B) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Mky, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

EIF4E3 antibody

EIF4E3 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

EIF4E3 antibody

EIF4E3 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

Lysates

Catalog No.    

EIF4E3 overexpression lysate

EIF4E3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

EIF4E3 overexpression lysate

EIF4E3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

EIF4E3 overexpression lysate

EIF4E3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

12 Item(s)

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