EIF4EBP1 / 4E-BP1 antibody

Principal name

EIF4EBP1 / 4E-BP1 antibody

Alternative names for EIF4EBP1 / 4E-BP1 antibody

PHAS-I, PHAS-1, PHAS1, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1

SwissProt ID

Q0P5A7 (Bovin), Q13541 (Human), Q60876 (Mouse), Q62622 (Rat)

Gene ID

1978 (EIF4EBP1), 71978 (Ppp2r2a)

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat), Mky (Monkey), Bov (Bovine), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Flow Cytometry (F), Immunoprecipitation (IP), Dot blot (Dot), Frozen Sections (C)

Background of EIF4EBP1 / 4E-BP1 antibody

PHAS-I, also known as eIF4E-BP1 and PHAS-II, -III (eIF4E-BP2, 3), are members of a family of proteins that regulate eukaryotic translation initiation which is mediated by the cap structure (m7GpppN, where N=any nucleotide) present at the 5'end of all cellular mRNAs, except organellar. The m7 cap is essential for the translation of most mRNA because it directs the translation machinery of the 5' end of the mRNA via its interaction with the cap binding protein, the translation initiation factor 4E(eIF4E). eIF4E plays an principal role in determining global translation rates because its interaction with the cap facilitates the binding of the ribosome to the mRNA. Consistent with this role, eIF4E is required for cell cycle progression, exhibits anti-apoptotic activity and when overexpressed transforms cells. Interaction with PHAS proteins prevents incorporation of eIF4E into an active translation initiation complex and inhibits cap-dependent translation. However, this inhibitory effect is alleviated following phosphorylation of the PHAS proteins by a P13K-dependent pathway, involving signaling by the antiapoptotic kinase Akt/PKB, as well as FRAP/mTOR. Rat PHAS-I has 117 amino acids with a apparent molecular weight of 22 kDa and is 93% identical to eIF-4E-BPI cloned from human placenta. PHAS-I and -II were found to have overlapping but different patterns of expression in tissues. PHAS-I is expressed in a wide variety of cell types with the highest being in two of the most insulin-responsive tissues, adipocytes and skeletal muscle. Both PHAS proteins are phosphorylated in response to insulin or growth factors such as EGF, PDGF and IGF-1. Increasing cAMP in cells promotes dephosphorylation of both PHAS-I and PHAS-II but that regulation of the two protein differ because PHAS-II, unlike PHAS-I is readily phosphorylated by PKA. The PHAS-I initiation factor has 2-8 phosphorylation sites and is multiply phosphorylated by insulinstimulated protein kinase(s) resulting in 8-10 phosphorylated isoforms in exponentially growing cells. Changes occur in the expression of these isoforms in response to stresses such as heat shock, and this may contribute to translation repression.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF4EBP1 / 4E-BP1 antibody

Western blot analysis of EIF4EBP1 (arrow) using rabbit polyclonal EIF4EBP1 Antibody (T69) (RB11407). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EIF4EBP1 gene (Lane 2) (Origene Technologies). Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF4EBP1 / 4E-BP1 antibody

Western blot analysis of 4E-BP1 in 3T3 cell lysate with 4E-BP1 antibody at (A) 2.5, (B) 5 and (C) 10 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

EIF4EBP1 / 4E-BP1 antibody

Predicted band size: 13kDa. Positive control: 293T cell lysate. Recommended dilution: 1/200-1000. (Gel: 8+12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/530 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4EBP1 / 4E-BP1 antibody

Predicted band size: 13kDa. Positive control: 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8+12%SDS-PAGE Lysate: 40 ug Primary antibody: 1/320 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/8000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4EBP1 / 4E-BP1 antibody

Western blot analysis of 4E-BP1 expression in EGF treated MDA-MB-435 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4EBP1 / 4E-BP1 antibody

Western blot analysis of 4E-BP1 expression in EGF treated MDA-MB-435 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4EBP1 / 4E-BP1 antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 4E-BP1 antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4EBP1 / 4E-BP1 antibody

Western blot analysis of extracts from Jurkat cells, treated with Insulin (0.01U/ml, 15mins), using 4E-BP1 (Ab-64) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4EBP1 / 4E-BP1 antibody

Western blot analysis of extracts from COS7 cells, treated with EGF (200ng/ml, 30mins), using 4E-BP1 (Ab-69) antibody (#TA326078).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4EBP1 / 4E-BP1 antibody

Western blot analysis using 4E-BP1 antibody Cat.-No AM06148SU-N against truncated 4E-BP1 recombinant protein (Lane 1) and A431 cell lysate (Lane 2). Mouse IgG1 9E12D9 Ascites Hu WB
0.1 ml / €360.00
  Acris Antibodies GmbH

EIF4EBP1 / 4E-BP1 antibody

Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue (C), showing cytoplasmic and membrane localization using 4E-BP1 antibody Cat.-No AM06149SU-N  with DAB staining. Mouse IgG1 4B6G10 Ascites Hu P, WB
0.1 ml / €480.00
  Acris Antibodies GmbH

EIF4EBP1 / 4E-BP1 antibody

Immunohistochemical analysis of paraffin-embedded human pancreas carcinoma (A), esophagus carcinoma tissue (B) and ovary tumor tissue, showing cytoplasmic and membrane localization using 4E-BP1 antibody Cat.-No AM06150PU-N with DAB staining. Mouse IgG1 11G12C11 Purified Hu P, WB
0.1 ml / €360.00
  Acris Antibodies GmbH

EIF4EBP1 / 4E-BP1 pThr45 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 antibody (AP02482PU-S; AP02482PU-N). Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

EIF4EBP1 / 4E-BP1 pThr45 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 antibody (AP02482PU-S; AP02482PU-N). Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €240.00
  Acris Antibodies GmbH

EIF4EBP1 / 4E-BP1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 antibody. Rabbit Aff - Purified Hu, Ms P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

EIF4EBP1 / 4E-BP1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 antibody. Rabbit Aff - Purified Hu, Ms P, WB
50 µl / €230.00
  Acris Antibodies GmbH

EIF4EBP1 / 4E-BP1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 antibody. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

EIF4EBP1 / 4E-BP1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1 antibody. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

EIF4EBP1 / 4E-BP1 antibody

Immunohistochemistry (IHC) analyzes of 4E-BP1 antibody (Cat.-No.: AP06364PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

EIF4EBP1 / 4E-BP1 antibody

Immunohistochemistry (IHC) analyzes of 4E-BP1 antibody (Cat.-No.: AP06365PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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