EIF4G1 / EIF4F antibody

Principal name

EIF4G1 / EIF4F antibody

Alternative names for EIF4G1 / EIF4F antibody

EIF4G, EIF4GI, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, Eukaryotic translation initiation factor 4 gamma 1, p220

SwissProt ID

P41110 (Rabit), Q04637 (Human), Q6NZJ6 (Mouse)

Gene ID

1981 (EIF4G1), 208643 (Eif4g1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Rb (Rabbit), Por (Porcine), Bov (Bovine), Eq (Equine), GP (Guinea Pig)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of EIF4G1 / EIF4F antibody

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. Interacts with eIF3, mutually exclusive with EIF4A1 or EIFA2, EIF4E and through its N-terminus with PAPBC1. Interacts through its C-terminus with the serine/threonine kinases MKNK1, and with MKNK2. Appears to act as a scaffold protein, holding these enzymes in place to phosphorylate EIF4E. Non-phosphorylated EIF4EBP1 competes with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. EIF4G1/EIF4G3 interacts with PABPC1 to bring about circularization of the mRNA. Rapamycin can attenuate insulin stimulation mediated by FKBPs. Interacts with EIF4E3. Interacts with MIF4GD. Interacts with rotavirus A NSP3; in this interaction, NSP3 takes the place of PABPC1 thereby inducing shutoff of host protein synthesis

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF4G1 / EIF4F antibody

  Anti-EIF4G1 (phospho-Ser1232) Rabbit Polyclonal Antibody  
Predicted band size: 220 kDa . Positive control: HT29 cells untreated or treated with Anisomycin lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: HT29 cells untreated with Anisomycin lysate Lane2: HT29 cells treated with Anisomycin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4G1 / EIF4F antibody

  Rabbit Polyclonal Anti-EIF4G1 Antibody  
WB Suggested Anti-EIF4G1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: HepG2 cell lysateEIF4G1 is supported by BioGPS gene expression data to be expressed in HepG2 Rabbit IgG Purified Bov, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

EIF4G1 / EIF4F antibody

  Rabbit Polyclonal Anti-EIF4G1 Antibody  
Gel: 6%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Lovo cells, 293T cells, Primary antibody: (EIF4G1 Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

EIF4G1 / EIF4F antibody

Immunohistochemistry (IHC) analysis of eIF4G antibody (Cat.-No.: AP06520PU-N) in paraffin-embedded human colon carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P
0.1 mg / €350.00
  Acris Antibodies GmbH

EIF4G1 / EIF4F pSer1232 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (phospho-Ser1232) antibody AP08034PU. Rabbit Aff - Purified Hu P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

EIF4G1 / EIF4F pSer1232 antibody

Figure 1. Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G (phospho-Ser1232) antibody AP08034PU. Rabbit Aff - Purified Hu P, WB
50 µl / €240.00
  Acris Antibodies GmbH

EIF4G1 / EIF4F antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G Antibody. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

EIF4G1 / EIF4F antibody

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using eIF4G Antibody. Rabbit Aff - Purified Hu ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

EIF4G1 / EIF4F pSer1108 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eIF4G (Phospho-Ser1108) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
50 µg / €230.00
  Assay Biotech

EIF4G1 / EIF4F pSer1108 antibody

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using eIF4G (Phospho-Ser1108) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P
0.1 mg / €330.00
  Assay Biotech

EIF4G1 / EIF4F antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using eIF4G (Ab-1108) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
50 µg / €230.00
  Assay Biotech

EIF4G1 / EIF4F antibody

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using eIF4G (Ab-1108) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, P
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

EIF4G1 - human gene knockout kit via CRISPR

EIF4G1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

EIF4G1 - human gene knockout kit via CRISPR

EIF4G1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

EIF4G1 - human gene knockout kit via CRISPR

EIF4G1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

EIF4G1 - human gene knockout kit via CRISPR

EIF4G1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EIF4G1 / EIF4F (transcript variant 5)

  Purified recombinant protein of Homo sapiens eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 5  
EIF4G1 / EIF4F Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EIF4G1 / EIF4F (transcript variant 2)

  Purified recombinant protein of Homo sapiens eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 2  
EIF4G1 / EIF4F Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

EIF4G1 overexpression lysate

EIF4G1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, EIF4G1 (mGFP-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, EIF4G1 (mGFP-tagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €2,530.00
  OriGene Technologies, Inc.

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