EIF5 antibody

Principal name

EIF5 antibody

Alternative names for EIF5 antibody

Eukaryotic translation initiation factor 5, eIF-5

SwissProt ID

P55010 (Human), P59325 (Mouse), Q07205 (Rat)

Gene ID

1983 (EIF5)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Chk (Chicken), Dros (Drosophila), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of EIF5 antibody

Eukaryotic initiation factor 5 (eIF5) plays an important role in the assembly of the 43S pre-initiation complex. In this assembly, eIF5 interacts with eIF1 and eIF3 and binds the eIF2-GTP/Met-tRNA ternary complex along with the 40S ribosome subunit. eIF5 is also reported to form a complex with eIF2. This complex is suggested to serve as a cytoplasmic reservoir for eIF2 that restricts the guanine nucleotide exchange promoted by the eIF2 beta subunit for the initiation of translation.

General readings

Olsen,J.V., (2006) Cell 127 (3), 635-648

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

EIF5 antibody

Immunoperoxidase of monoclonal antibody to EIF5 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 1ug/ml] Mouse IgG1 2E6-4C12 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EIF5 antibody

WB Suggested Anti-EIF5 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysateThere is BioGPS gene expression data showing that EIF5 is expressed in HeLa Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

EIF5 antibody

Western Blot analysis of EIF5 expression in transfected 293T cell line by EIF5 MaxPab rabbit polyclonal antibody.

Lane 1: EIF5 transfected lysate(49.20 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

EIF5 antibody

EIF5 Mouse IgG1 1D9-4B9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

EIF5 antibody

Human Hela; Host: Rabbit . Target Name: EIF5 . Sample Tissue: Hela . Antibody Dilution: 1.0ug/ml. There is BioGPS gene expression data showing that EIF5 is expressed in HeLa.; EIF5 antibody - N-terminal region (ARP53443_P050) in Human Hela cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Dros, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

EIF5 antibody

Western Blot: eIF5 Antibody [NB100-93312] - Detection of Human and Mouse eIF5 by Western Blot.  Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for  WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and  mouse NIH3T3 (M; 50 mcg) cells. Antibody: Affinity purified  rabbit anti-eIF5 antibody NB100-93312 used for WB at 0.04  mcg/ml. Rabbit IgG Aff - Purified Hu, Ms WB
0.1 ml / €440.00
  Novus Biologicals Inc.

EIF5 antibody

Immunohistochemistry-Paraffin: EIF5 Antibody [NBP1-86184] - Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells. Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

EIF5 (transcript variant 1)

EIF5 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

EIF5

EIF5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

EIF5

EIF5 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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