ELMO1 antibody

Principal name

ELMO1 antibody

Alternative names for ELMO1 antibody

ELMO 1, ELMO-1, CED-12 homolog, KIAA0281, Engulfment and cell motility protein 1

SwissProt ID

Q8BPU7 (Mouse), Q92556 (Human)

Gene ID

9844 (ELMO1), 140580 (Elmo1)

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Ze (Zebrafish), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of ELMO1 antibody

Kit Component:
- KN305159G1, Elmo1 gRNA vector 1 in pCas-Guide vector
- KN305159G2, Elmo1 gRNA vector 2 in pCas-Guide vector
- KN305159D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector
CRISPR Manual:
CRISPR/CAS9 Genome Editing Manual

General readings

1. Epting D, Wendik B, Bennewitz K, Dietz CT, Driever W, Kroll J. The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish. Circ Res. 2010 Jul 9;107(1):45-55. PMID: 20466982
2. Sai J, Raman D, Liu Y, Wikswo J, Richmond A. Parallel phosphatidylinositol 3-kinase (PI3K)-dependent and Src-dependent pathways lead to CXCL8-mediated Rac2 activation and chemotaxis. J Biol Chem. 2008 Sep 26;283(39):26538-47.
PMID: 18662984
3. Jarzynka MJ, Hu B, Hui KM, Bar-Joseph I, Gu W, Hirose T, Haney LB, Ravichandran KS, Nishikawa R, Cheng SY. ELMO1 and Dock180, a bipartite Rac1 guanine nucleotide exchange factor, promote human glioma cell invasion. Cancer Res. 2007 Aug 1;67(15):7203-11. PMID: 17671188
4. Makino Y, Tsuda M, Ichihara S, Watanabe T, Sakai M, Sawa H, Nagashima K, Hatakeyama S, Tanaka S. Elmo1 inhibits ubiquitylation of Dock180. J Cell Sci. 2006 Mar 1;119(Pt 5):923-32. PMID: 16495483
5. Côté JF, Motoyama AB, Bush JA, Vuori K. A novel and evolutionarily conserved PtdIns(3,4,5)P3-binding domain is necessary for DOCK180 signalling. Nat Cell Biol. 2005 Aug;7(8):797-807. Epub 2005 Jul 17. PMID: 16025104

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ELMO1 (C-term) antibody

  Goat Polyclonal Antibody against ELMO1  
TA302474 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt, Ze E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ELMO1 antibody

  Rabbit Polyclonal Anti-ELMO1 Antibody  
WB Suggested Anti-ELMO1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

ELMO1 - human gene knockout kit via CRISPR

ELMO1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

ELMO1 - human gene knockout kit via CRISPR

ELMO1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

ELMO1 - human gene knockout kit via CRISPR

ELMO1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Elmo1 - mouse gene knockout kit via CRISPR

Elmo1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Elmo1 - mouse gene knockout kit via CRISPR

Elmo1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Elmo1 - mouse gene knockout kit via CRISPR

Elmo1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Elmo1 - mouse gene knockout kit via CRISPR

Elmo1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ELMO1 (transcript variant 1)

  Recombinant protein of human engulfment and cell motility 1 (ELMO1), transcript variant 1  
ELMO1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ELMO1 (transcript variant 3)

  Recombinant protein of human engulfment and cell motility 1 (ELMO1), transcript variant 3  
ELMO1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ELMO1 (full length, N-term HIS tag, transcript variant 2)

  Purified recombinant protein of Human engulfment and cell motility 1 (ELMO1), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
ELMO1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

ELMO1 overexpression lysate

ELMO1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

ELMO1 overexpression lysate

ELMO1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, ELMO1 (mGFP-tagged) - Human engulfment and cell motility 1 (ELMO1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, ELMO1 (mGFP-tagged) - Human engulfment and cell motility 1 (ELMO1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, ELMO1 (mGFP-tagged) - Human engulfment and cell motility 1 (ELMO1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, ELMO1 (mGFP-tagged) - Human engulfment and cell motility 1 (ELMO1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, ELMO1 (Myc-DDK tagged) - Human engulfment and cell motility 1 (ELMO1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, ELMO1 (Myc-DDK tagged) - Human engulfment and cell motility 1 (ELMO1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Lenti ORF particles, ELMO1 (Myc-DDK tagged) - Human engulfment and cell motility 1 (ELMO1), transcript variant 2 , 200 uL, >10^7 TU/mL

Lenti ORF particles, ELMO1 (Myc-DDK tagged) - Human engulfment and cell motility 1 (ELMO1), transcript variant 2 , 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Elmo1 (GFP-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Elmo1 (GFP-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,410.00
  OriGene Technologies, Inc.

Lenti ORF particles, Elmo1 (Myc-DDK-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Elmo1 (Myc-DDK-tagged) - Mouse engulfment and cell motility 1, ced-12 homolog (C. elegans) (Elmo1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,270.00
  OriGene Technologies, Inc.

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