ELMO1 antibody

Principal name

ELMO1 antibody

Alternative names for ELMO1 antibody

ELMO 1, ELMO-1, CED-12 homolog, KIAA0281, Engulfment and cell motility protein 1

SwissProt ID

Q8BPU7 (Mouse), Q92556 (Human)

Gene ID

9844 (ELMO1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Ze (Zebrafish), Can (Canine), Rt (Rat), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunoprecipitation (IP), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection))

Background of ELMO1 antibody

The protein encoded by this gene interacts with the dedicator of cyto-kinesis 1 protein to promote phagocytosis and effect cell shape changes. Similarity to a C. elegans protein suggests that this protein may function in apoptosis and in cell migration. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq]. Transcript Variant: This variant (2) includes a unique exon in its 5' end, resulting in use of a downstream in-frame start codon as compared to variant 1. Isoform 2 is 480 aa shorter than isoform 1. Transcript variants 2 and 3 encoded the same protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

1. Epting D, Wendik B, Bennewitz K, Dietz CT, Driever W, Kroll J. The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish. Circ Res. 2010 Jul 9;107(1):45-55. PMID: 20466982
2. Sai J, Raman D, Liu Y, Wikswo J, Richmond A. Parallel phosphatidylinositol 3-kinase (PI3K)-dependent and Src-dependent pathways lead to CXCL8-mediated Rac2 activation and chemotaxis. J Biol Chem. 2008 Sep 26;283(39):26538-47.
PMID: 18662984
3. Jarzynka MJ, Hu B, Hui KM, Bar-Joseph I, Gu W, Hirose T, Haney LB, Ravichandran KS, Nishikawa R, Cheng SY. ELMO1 and Dock180, a bipartite Rac1 guanine nucleotide exchange factor, promote human glioma cell invasion. Cancer Res. 2007 Aug 1;67(15):7203-11. PMID: 17671188
4. Makino Y, Tsuda M, Ichihara S, Watanabe T, Sakai M, Sawa H, Nagashima K, Hatakeyama S, Tanaka S. Elmo1 inhibits ubiquitylation of Dock180. J Cell Sci. 2006 Mar 1;119(Pt 5):923-32. PMID: 16495483
5. Côté JF, Motoyama AB, Bush JA, Vuori K. A novel and evolutionarily conserved PtdIns(3,4,5)P3-binding domain is necessary for DOCK180 signalling. Nat Cell Biol. 2005 Aug;7(8):797-807. Epub 2005 Jul 17. PMID: 16025104

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

ELMO1 (C-term) antibody

TA302474 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt, Ze E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

ELMO1 antibody

WB Suggested Anti-ELMO1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

ELMO1 antibody

Western Blot analysis of ELMO1 expression in transfected 293T cell line (H00009844-T01) by ELMO1 MaxPab polyclonal antibody.

Lane 1: ELMO1 transfected lysate(79.97 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

ELMO1 antibody

ELMO1 polyclonal antibody ( Cat # PAB6103 ) staining ( 1 µg/ml ) of Jurkat lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Bov, Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

ELMO1 (C-term) antibody

ELMO1 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

ELMO1 antibody

Western blot analysis of ELMO1 expression in Jurkat (A); H1299 (B); HeLa (C); HL60 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

ELMO1 antibody

Western blot analysis of ELMO1 expression in Jurkat (A); H1299 (B); HeLa (C); HL60 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

ELMO1 antibody

Western blot analysis of ELMO1 expression in Jurkat (A); H1299 (B); HeLa (C); HL60 (D) whole cell lysates. Rabbit Aff - Purified Hu, Ms, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

ELMO1 antibody

NB 100-875 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Bov, Hu, Ze E, IP, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

ELMO1 antibody

Western  Blot:  ELMO1 Antibody [NBP1-33645] – Western blot analysis of ELMO1 on H1299 lysates using NBP1-33645 at a dilution of 1:1000. 30ug whole cell lysate loaded on 12% SDS PAGE gel. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

ELMO1 antibody

Immunohistochemistry-Paraffin: ELMO1 Antibody [NBP1-85696] - Immunohistochemical staining of human stomach shows strong positivity in Chief cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

ELMO1 (transcript variant 1)

ELMO1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ELMO1 (transcript variant 3)

ELMO1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

ELMO1 (full length, N-term HIS tag, transcript variant 2)

ELMO1 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

ELMO1

ELMO1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

ELMO1

ELMO1 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

ELMO1

ELMO1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

ELMO1

ELMO1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

ELMO1 Lysate(Denatured)

ELMO1 Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

ELMO1 overexpression lysate

ELMO1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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