Endophilin-B1 antibody

Principal name

Endophilin-B1 antibody

Alternative names for Endophilin-B1 antibody

SH3GLB1, KIAA0491, BIF1, Bif-1, SH3 domain-containing GRB2-like protein B1, Bax-interacting factor 1

SwissProt ID

Q32LM0 (Bovin), Q5ZIR1 (Chick), Q6AYE2 (Rat), Q9JK48 (Mouse), Q9Y371 (Human)

Gene ID

51100 (SH3GLB1), 54673 (Sh3glb1), 292156

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP)

Background of Endophilin-B1 antibody

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes.. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.

General readings

Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ, Wang HG. Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis.
Mol Cell Biol. 2005 Nov;25(21):9369-82.
PMID: 16227588

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Endophilin-B1 antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1B3-A5 Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Endophilin-B1 (C-term) antibody

  Goat Polyclonal Antibody against SH3GLB1  
TA302726 staining (0.3µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endophilin-B1 antibody

  Rabbit Polyclonal Bif Antibody  
Western blot analysis of BIF in HeLa cell lysate with BIF antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endophilin-B1 antibody

  Mouse Monoclonal Bif-1/Endophilin-B1 Antibody (30A882.1.1)  
Western Blot: Bif-1/Endophilin-B1 Antibody (30A882.1.1) [TA337048] - Analysis for Bi-f-1/Endophilin-B1 using this antibody at 2 ug/ml dilution against 10 ug of Jurkat cell lysate. Mouse IgG1 30A882.1.1 Purified Hu C, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endophilin-B1 antibody

  Rabbit Polyclonal Anti-SH3GLB1 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human gastic cancer tissue using (SH3GLB1 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Endophilin-B1 antibody

Figure 2. Formalin-Fixed Paraffin-Embedded (FFPE) on Heart. Rabbit Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Endophilin-B1 antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Endophilin-B1 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Endophilin B1 Antibody (Y80), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

SH3GLB1 - human gene knockout kit via CRISPR

SH3GLB1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Sh3glb1 - mouse gene knockout kit via CRISPR

Sh3glb1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Sh3glb1 - mouse gene knockout kit via CRISPR

Sh3glb1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sh3glb1 - mouse gene knockout kit via CRISPR

Sh3glb1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Sh3glb1 - mouse gene knockout kit via CRISPR

Sh3glb1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Endophilin-B1

  Recombinant protein of human SH3-domain GRB2-like endophilin B1 (SH3GLB1)  
Endophilin-B1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Endophilin-B1 (1-365, His-tag)

Endophilin-B1 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

Endophilin-B1 (1-365, His-tag)

Endophilin-B1 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

SH3GLB1 overexpression lysate

SH3GLB1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, SH3GLB1 (mGFP-tagged) - Human SH3-domain GRB2-like endophilin B1 (SH3GLB1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, SH3GLB1 (mGFP-tagged) - Human SH3-domain GRB2-like endophilin B1 (SH3GLB1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, SH3GLB1 (Myc-DDK tagged) - Human SH3-domain GRB2-like endophilin B1 (SH3GLB1), transcript variant 1 , 200 uL, >10^7 TU/mL

Lenti ORF particles, SH3GLB1 (Myc-DDK tagged) - Human SH3-domain GRB2-like endophilin B1 (SH3GLB1), transcript variant 1 , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Sh3glb1 (GFP-tagged) - Mouse SH3-domain GRB2-like B1 (endophilin) (Sh3glb1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Sh3glb1 (GFP-tagged) - Mouse SH3-domain GRB2-like B1 (endophilin) (Sh3glb1), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

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