Endophilin-B1 antibody

Principal name

Endophilin-B1 antibody

Alternative names for Endophilin-B1 antibody

SH3GLB1, KIAA0491, BIF1, Bif-1, SH3 domain-containing GRB2-like protein B1, Bax-interacting factor 1

SwissProt ID

Q32LM0 (Bovin), Q5ZIR1 (Chick), Q6AYE2 (Rat), Q9JK48 (Mouse), Q9Y371 (Human)

Gene ID

51100 (SH3GLB1), 54673 (Sh3glb1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of Endophilin-B1 antibody

The Bcl-2 family consists of anti-apoptotic (or cell survival) genes, such as Bcl-2, Bcl-XL and pro-apoptotic (or cell death) genes including Bax and BAD. In addition to its anti-apoptotic effect, Bcl-2 also inhibits progression through the cell cycle. Functional interactions between family members as well as binding to other cellular proteins modulate their activities. Bcl-2 family members are important in normal tissue development, homeostasis, and disease states. Bcl-2 prevents cell death by forming heterodimers with Bax and Bad (1). Bax promotes cell death by forming homodimers, which can be blocked by forming heterodimers with Bcl-2 or BCL-XL. Recently, using yeast two-hybrid system Cuddeback et al have identified a cDNA encoding a 365 amino acid protein, which interacts physically with Bax protein and influences cell life and death. Bif-1 contains a Src homology 3 (SH3) domain in the C-terminus. It does not contain any of the conserved Bcl-2 homology (BH) domains of the Bcl-2 family proteins. Human Bif-1 protein shares 96% and 42% identity at the amino acids level with mouse and C. elegans, respectively. Overexpression of Bif-1 in transfected cell lines promotes Bax conformational change, caspase activation, and apoptotic cell death following growth factor withdrawal. Bif-1 RNA is abundantly expressed in heart, skeletal muscle, kidney, and placenta.

General readings

Takahashi Y, Karbowski M, Yamaguchi H, Kazi A, Wu J, Sebti SM, Youle RJ, Wang HG. Loss of Bif-1 suppresses Bax/Bak conformational change and mitochondrial apoptosis.
Mol Cell Biol. 2005 Nov;25(21):9369-82.
PMID: 16227588

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Endophilin-B1 antibody

Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 1B3-A5 Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

Endophilin-B1 antibody

Immunoperoxidase of monoclonal antibody to SH3GLB1 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 3ug/ml] Mouse IgG1 1B3-A5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Endophilin-B1 (C-term) antibody

TA302726 staining (0.3µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endophilin-B1 antibody

Western blot analysis of BIF in HeLa cell lysate with BIF antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endophilin-B1 antibody

Western Blot: Bif-1/Endophilin-B1 Antibody (30A882.1.1) [TA337048] - Analysis for Bi-f-1/Endophilin-B1 using this antibody at 2 ug/ml dilution against 10 ug of Jurkat cell lysate. Mouse IgG1 30A882.1.1 Purified Hu C, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endophilin-B1 antibody

Figure 2. Formalin-Fixed Paraffin-Embedded (FFPE) on Heart. Rabbit Purified Hu, Ms ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Endophilin-B1 antibody

Skeletal muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Endophilin-B1 antibody

Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Endophilin B1 Antibody (Y80), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Endophilin-B1 Control Peptide

Endophilin-B1
50 µg / €160.00
  Acris Antibodies GmbH

Endophilin-B1 antibody

Western Blot analysis of SH3GLB1 expression in transfected 293T cell line (H00051100-T01) by SH3GLB1 MaxPab polyclonal antibody.

Lane 1: SH3GLB1 transfected lysate(40.15 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

Endophilin-B1 antibody

Western Blot analysis of SH3GLB1 expression in transfected 293T cell line (H00051100-T02) by SH3GLB1 MaxPab polyclonal antibody.

Lane 1: SH3GLB1 transfected lysate(40.80 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Endophilin-B1 antibody

SH3GLB1 polyclonal antibody ( Cat # PAB6051 ) staining ( 0.3 µg/ml ) of Jurkat lysate ( RIPA buffer, 35 µg total protein per lane ) . Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Endophilin-B1 antibody

Immunocytochemistry of SH3GLB1 in HeLa with SH3GLB1 polyclonal antibody ( Cat # PAB12961 ) at 10 µg/ml. Rabbit Hu, Ms ICC/IF, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

Endophilin-B1 (N-term) antibody

Mouse Thymus; WB Suggested Anti-Sh3glb1 Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Thymus; Sh3glb1 antibody - N-terminal region (ARP58875_P050) in Mouse Thymus cells using Western Blot Rabbit Aff - Purified Hu, Ms WB
50 µg / €350.00
  AVIVA Systems Biology

Endophilin-B1 antibody

Western Blot: Bif Antibody [NB100-820] - NB100-820 staining (0.3ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

Endophilin-B1 antibody

Endophilin-B1 Rabbit Hu, Ms ICC/IF, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

Endophilin-B1 antibody

Immunofluorescence: Bif-1 Antibody [NBP1-31130] - Immunofluorescence analysis of A431, using Bif antibody at 1:200 dilution. Rabbit IgG Hu, Ms ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Endophilin-B1 antibody

Immunohistochemistry-Paraffin: PCMT1 Antibody [NBP1-85806] - Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Endophilin-B1 antibody

Immunohistochemistry-Paraffin: Bif-1 Antibody [NBP1-89972] - Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Endophilin-B1

Endophilin-B1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

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