Endoplasmin / HSP90B1 / TRA1 antibody

Principal name

Endoplasmin / HSP90B1 / TRA1 antibody

Alternative names for Endoplasmin / HSP90B1 / TRA1 antibody

Tumor rejection antigen 1, GRP94, Heat shock protein 90 kDa beta member 1, 94 kDa glucose-regulated protein

SwissProt ID

O18750 (Rabit), P08110 (Chick), P08113 (Mouse), P14625 (Human), P41148 (Canfa), Q29092 (Pig), Q66HD0 (Rat), Q95M18 (Bovin)

Gene ID

7184 (HSP90B1), 374163, 22027 (Hsp90b1)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), GP (Guinea Pig), Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Sh (Sheep), Xen (Xenopus), Ze (Zebrafish), African clawed frog, Dros (Drosophila), Chimp (Chimpanzee), Eq (Equine), Av (Birds)

Available hosts

Rat, Rabbit, Mouse, Goat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Flow Cytometry (F), Frozen Sections (C), ELISA (detection) (E(detection))

Background of Endoplasmin / HSP90B1 / TRA1 antibody

Glucose-regulated protein 94, also known as Grp94 or gp96, is an abundant resident endoplasmic reticulum (ER) lumenal stress protein which together with cytosolic Hsp90 belongs to the Hsp90 family of molecular chaperones. Grp94 and other resident soluble proteins of the ER such as members of the Ca(2+) binding protein subfamily (CaBP), CaBPI and CaBP2 as well as calreticulin, possess the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL) which is a sorting signal that is thought to lead to the retention of these proteins in the pre-Golgi compartments (1). Grp94 expression is upregulated by stress conditions such as lucose starvation and heat shock, which promote protein misfolding or unfolding (2). In addition to a homeostatic role in protein folding and assembly, Grp94 can function in the intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen presentation cells (3,4). Grp94 and Hsp90 share high sequence identity and presumably identical adenosine nucleotide-dependent modes of regulation. Earlier data suggests that Hsp90 and Grp94 may differ in their nucleotide binding properties. The N-terminal domain of eukaryotic Hsp90 proteins contains a conserved adenosine nucleotide binding pocket which also serves as the inding site for the Hsp90 inhibitors geldanamycin and radicicol. However, the molecular basis for adenosine nucleotide-dependent regulation of Grp94remains to be established. Recent data has entified a ligand dependent regulation of Grp94 function and suggest a model whereby Grp94 function is regulated through a ligand-dependent conversion of Grp94 from an inactive to an active conformation (5, 6).

General readings

This product has been used in:Gething MJ & Sambrook J Protein folding in the cell. Nature 355:33-45 (1992). PubMed PMID: 1731198
Kulomaa MS et al. Amino acid sequence of a chicken heat shock protein derived from the complementary DNA nucleotide sequence. Biochemistry 25:6244-51 (1986). PubMed PMID: 3024703
Sargan DR et al. hsp108, a novel heat shock inducible protein of chicken. Biochemistry 25:6252-8 (1986). PubMed PMID: 3098286
Edwards DP et al. Structural analysis of chicken oviduct progesterone receptor using monoclonal antibodies to the subunit B protein. Biochemistry 23:4427-35 (1984). PubMed PMID: 6207857
General / background references:Nauseef WM et al. Calreticulin functions as a molecular chaperone in the biosynthesis of myeloperoxidase. J Biol Chem 270:4741-7 (1995). PubMed PMID: 7876246

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Endoplasmin / HSP90B1 / TRA1 (676-803) antibody

Immunohistochemistry: AM09037PU-N HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG2a 2H3 Purified Hu E, ICC/IF, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 (676-803) antibody

Immunohistochemistry: AM09037PU-N HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG2a 2H3 Purified Hu E, ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 antibody

Prostate, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Ms IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 (C-term) antibody

AP15588PU GRP94 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 (C-term) antibody

AP15588PU GRP94 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P, WB
1 ml / €520.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 (C-term) antibody

AP15588PU GRP94 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 antibody

TA303118 (0.03µg/ml) staining of 3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Ze E, P, WB
20 µg / €200.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 antibody

TA303118 (0.03µg/ml) staining of 3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Ze E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

Western blot analysis of extracts from SKOV3 cells, using GRP94 antibody (#TA313019).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

Predicted band size: 92 kDa . Positive control: Hela cell and human liver cancer tissue, Jurkat and NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: Hhuman liver cancer tissue lysate Lane3: Jurkat cell lysate Lane4: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

Predicted band size: 92 kDa . Positive control: Hela cell and human liver cancer tissue, Jurkat and NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: Human liver cancer tissue lysate Lane3: Jurkat cell lysate Lane4: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 (Center) antibody

HSP90B1 Antibody (Center) (Cat.# TA324447) western blot analysis in Hela,MCF-7,mouse C2C12 cell line lysates (35ug/lane).This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow). Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

HSP90B1 Antibody (N-term) (Cat. #TA324795) western blot analysis in HepG2, CHO, K562,  Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow). Rabbit IgG Purified Bov, Chk, Hst, Hu, Mky, Ms, Por, Rt F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

Western blot analysis of Grp94 using Hela cell lysate at 1:1000 dilution of the antibody Rat IgG2a 9G10 Aff - Purified Bov, Can, Chk, Eq, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen F, IP, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

WB of rat brain lysate showing detection of ~94 kDa GRP94 using Anti-GRP94. Load: 20 µg RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rabbit Anti-GRP94 polyclonal antibody incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50-1:100 for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Predicted/Observed size: ~ 94-100 kDa. Other band(s): ~50, ~75 kDa. Rabbit Purified Bov, Hu, Ms, Rt IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

HeLa cell extracts were resolved by electrophoresis, transferred to nitrocelluose, and probed with purified polyclonal anti-GRP94 antibody. Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection. Rabbit IgG Poly6499 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

Western blot analysis on SKOV3 cell lysate using GRP94 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

Western blot analysis of Grp94 using Hela cell lysate at 1:1000 dilution of the antibody Rat IgG2a 9G10 Aff - Purified Bov, Can, Chk, Eq, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen F, IP, WB
50 µg / €210.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 antibody

The Recombinant Human GRP94 (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: Recombinant Human GRP94
Lane 2.: HeLa cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: HepG2 cell lysate Mouse AT94B9 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 antibody

The Recombinant Human GRP94 (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: Recombinant Human GRP94
Lane 2.: HeLa cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: HepG2 cell lysate Mouse AT94B9 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

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