Endoplasmin / HSP90B1 / TRA1 antibody

Principal name

Endoplasmin / HSP90B1 / TRA1 antibody

Alternative names for Endoplasmin / HSP90B1 / TRA1 antibody

Tumor rejection antigen 1, GRP94, Heat shock protein 90 kDa beta member 1, 94 kDa glucose-regulated protein

SwissProt ID

O18750 (Rabit), P08110 (Chick), P08113 (Mouse), P14625 (Human), P41148 (Canfa), Q29092 (Pig), Q66HD0 (Rat), Q95M18 (Bovin)

Gene ID

7184 (HSP90B1), 374163, 22027 (Hsp90b1), 362862

Available reactivities

Bov (Bovine), Hu (Human), Mky (Monkey), Por (Porcine), Sh (Sheep), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Dros (Drosophila), Eq (Equine), Xen (Xenopus), Av (Birds), GP (Guinea Pig), Hst (Hamster), Rb (Rabbit), Bt (Bat), Fi (Fish), Op (Opossum), Prim (Primate), Tr (Turkey), L (Lizard), Bear

Available hosts

Rabbit, Goat, Mouse, Rat

Available applications

Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Endoplasmin / HSP90B1 / TRA1 antibody

Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2).
Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homolgy with its cytosolic equivalent, Hsp90 (3). Both Hsp90 and Grp94 are calcium binding proteins (4). Despite sharing 50% sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5).
Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).

General readings

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Suzuki, S., et al. Biochem. Biophys. Res. Commun. 398(3):525-531(2010)
Bloor, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(15):6970-6975(2010)
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Wang, X., et al. Clin. Dev. Immunol. 2010, 212537 (2010)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Endoplasmin / HSP90B1 / TRA1 (676-803) antibody

Immunohistochemistry: AM09037PU-N HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG2a 2H3 Purified Hu E, ICC/IF, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 (676-803) antibody

Immunohistochemistry: AM09037PU-N HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen. Mouse IgG2a 2H3 Purified Hu E, ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 antibody

Prostate, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Ms IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 (C-term) antibody

AP15588PU GRP94 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 (C-term) antibody

AP15588PU GRP94 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P, WB
1 ml / €530.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 (C-term) antibody

AP15588PU GRP94 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu P, WB
0.1 ml / €200.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 antibody

  Goat Polyclonal Antibody against HSP90B1  
TA303118 (0.03µg/ml) staining of 3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Ze E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

  Rabbit polyclonal anti-GRP94 antibody  
Western blot analysis of extracts from SKOV3 cells, using GRP94 antibody (#TA313019).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified E, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

  Anti-HSP90B1 Rabbit Polyclonal Antibody  
Predicted band size: 92 kDa . Positive control: Hela cell and human liver cancer tissue, Jurkat and NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: Hhuman liver cancer tissue lysate Lane3: Jurkat cell lysate Lane4: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

  Anti-HSP90B1 Rabbit Polyclonal Antibody  
Predicted band size: 92 kDa . Positive control: Hela cell and human liver cancer tissue, Jurkat and NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: Human liver cancer tissue lysate Lane3: Jurkat cell lysate Lane4: NIH/3T3 cell lysate Lysates: 40 ug per lane Primary antibody: 1/900 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 (Center) antibody

  Rabbit polyclonal HSP90B1 Antibody (Center)  
HSP90B1 Antibody (Center) (Cat.# TA324447) western blot analysis in Hela,MCF-7,mouse C2C12 cell line lysates (35ug/lane).This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow). Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

  Rabbit polyclonal HSP90B1 Antibody (N-term)  
HSP90B1 Antibody (N-term) (Cat. #TA324795) western blot analysis in HepG2, CHO, K562,  Hela cell line and mouse liver tissue lysates (35ug/lane).This demonstrates the HSP90B1 antibody detected the HSP90B1 protein (arrow). Rabbit IgG Purified Bov, Chk, Hst, Hu, Mky, Ms, Por, Rt F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

  Mouse monoclonal GRP94 Antibody  
Western blot analysis of Grp94 using Hela cell lysate at 1:1000 dilution of the antibody Rat IgG2a 9G10 Aff - Purified Bov, Can, Chk, Eq, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh, Xen F, IP, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

  Rabbit Polyclonal Anti-Grp94 Antibody  
WB of rat brain lysate showing detection of ~94 kDa GRP94 using Anti-GRP94. Load: 20 µg RB lysate per lane. Block: 5% milk + TBST 1hr at RT. Primary antibody: Rabbit Anti-GRP94 polyclonal antibody incubated for 60 min at RT. Secondary antibody: Goat Anti-Mouse HRP antibody at 1:50-1:100 for 60 min at RT. Colour Development: TMB solution for 5 min at RT. Predicted/Observed size: ~ 94-100 kDa. Other band(s): ~50, ~75 kDa. Rabbit Purified Bov, Hu, Ms, Rt IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

  Rabbit Polyclonal anti-GRP94 Antibody  
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocelluose, and probed with purified polyclonal anti-GRP94 antibody. Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection. Rabbit IgG Poly6499 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

  Rabbit Polyclonal Anti-GRP94 Antibody  
Western blot analysis on SKOV3 cell lysate using GRP94 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

  Rabbit Polyclonal anti-GRP94 Antibody  
HeLa cell extracts were resolved by electrophoresis, transferred to nitrocelluose, and probed with purified polyclonal anti-GRP94 antibody. Proteins were visualized using a donkey anti-rabbit-IgG secondary conjugated to HRP and chemiluminescence detection. Rabbit IgG Poly6499 Purified Hu WB
25 µl / €280.00
  OriGene Technologies, Inc.

Endoplasmin / HSP90B1 / TRA1 antibody

The Recombinant Human GRP94 (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: Recombinant Human GRP94
Lane 2.: HeLa cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: HepG2 cell lysate Mouse AT94B9 Purified Hu E, F, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 antibody

The Recombinant Human GRP94 (25ng) and Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GRP94 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Lane 1.: Recombinant Human GRP94
Lane 2.: HeLa cell lysate
Lane 3.: Jurkat cell lysate
Lane 4.: HepG2 cell lysate Mouse AT94B9 Purified Hu E, F, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

Endoplasmin / HSP90B1 / TRA1 antibody

Immunohistochemistry (IHC) analyzes of GRP94/HSP90B1 antibody (Cat.-No.: AP06150PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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