engrailed-1 / EN1 antibody

Principal name

engrailed-1 / EN1 antibody

Alternative names for engrailed-1 / EN1 antibody

Homeobox protein engrailed-1

SwissProt ID

P09065 (Mouse), Q05916 (Chick), Q05925 (Human)

Gene ID

2019 (EN1)

Available reactivities

Hu (Human), Ms (Mouse), Chk (Chicken), Ze (Zebrafish), African clawed frog, Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Frozen Sections (C)

Background of engrailed-1 / EN1 antibody

Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the \'engrailed\' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the \'engrailed\' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Atit,R., (2006) Dev. Biol. 296 (1), 164-176

15 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

engrailed-1 / EN1 (N-term) antibody

Western blot analysis of lysates from NCI-H460,293,SH-SY5Y cell line (from left to right),using EN1 Antibody (N-term)(Cat. #TA324916). TA324916 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

engrailed-1 / EN1 antibody

WB Suggested Anti-EN1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

engrailed-1 / EN1 antibody

WB Suggested Anti-EN1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

engrailed-1 / EN1 (266-392) antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3H2 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

engrailed-1 / EN1 antibody

Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 1F5 Purified Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

engrailed-1 / EN1 antibody

engrailed-1 / EN1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

engrailed-1 / EN1 antibody

Detection limit for recombinant GST tagged EN1 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 3E5 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

engrailed-1 / EN1 antibody

Immunoperoxidase of monoclonal antibody to EN1 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 3 ug/ml] Mouse IgG2a 3H2 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

engrailed-1 / EN1 antibody

Immunoperoxidase of monoclonal antibody to EN1 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 3 ug/ml] Mouse IgG2a 1F5 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

engrailed-1 / EN1 antibody

Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with EN1 polyclonal antibody ( Cat # PAB2058 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

engrailed-1 / EN1 antibody

Western blot analysis of EN1 polyclonal antibody ( Cat # PAB2059 ) in mouse kidney tissue lysates ( 35 µg/lane ) . EN1 ( arrow ) was detected using the purified Polyclonal antibody ( 1 : 60 dilution ) . Rabbit Hu, Ms E, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

engrailed-1 / EN1 antibody

Human THP-1; WB Suggested Anti-EN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: THP-1 cell lysate; EN1 antibody - middle region (ARP38029_P050) in Human THP-1 cells using Western Blot Rabbit Aff - Purified African clawed frog, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

engrailed-1 / EN1 antibody

Human HepG2; WB Suggested Anti-EN1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; EN1 antibody - C-terminal region (P100923_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified African clawed frog, Chk, Hu, Ms, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

engrailed-1 / EN1 antibody

engrailed-1 / EN1 Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Lysates (Overexpressed)

Catalog No.    

EN1 overexpression lysate

EN1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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